CORE--HISTOCHEMISTRY CORE

核心--组织化学核心

• 批准号: 5212845
• 负责人: RICHARD B SIMERLY
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5212845
• 关键词: biomedical facility; histochemistry /cytochemistry; immunocytochemistry; in situ hybridization; nucleic acid probes

The goal of the histochemistry core is to provide technical expertise, help with experimental design, assistance with anatomical image analysis, and training opportunities in modern histochemical methodology. Histochemistry methods are an important part of projects 1-4 of this program project. A core laboratory will provide facilities and technical assistance in the histological and histochemical processing of animal tissues and obviate the purchasing of expensive equipment required for these procedures by each laboratory. The core will also provide the necessary expertise in trouble-shooting histochemical experiments, as well assist with the analysis and quantification of the results. By Centralizing the tissue processing and coordinating experimental protocols used by each laboratory this core will facilitate progress toward the stated goals of each project and enhance the process of interrelating results across projects. Dr. Chinkers (Project 1) will utilize the histochemistry core to evaluate the distribution of natriuretic peptides in the hypothalamus and study their possible co- expression in CRF neurons, as well as define the expression pattern of receptors for these peptides in the hypothalamus and pituitary. Dr. Low (Project 2) will need free access to the core facilities to characterize the development and adult organization of key aspects of hypothalamic and pituitary components of the HPA axis in transgenic mice that lack beta- endorphin. Dr. Thomas (Project 3) proposes studies that will required access to the histochemistry core for processing pituitary tissue from transgenic animals for immunohistochemistry and in situ hybridization. Dr. Cone (project 4) will require extensive use of the core facilities to study the expression pattern of novel melanocortin receptors in the brain of adult and developing normal and transgenic animals. In support of these projects the histochemistry core will: 1) Provide equipment, supplies and technical assistance for the acquisition of frozen tissue sections using both cryostat and sliding microtomes; 2) Store frozen tissue samples (-cT) and slide-mounted sections until they can be processed for histochemistry; 3) Supply detailed protocols for immunohistochemical (both double fluorescence and ABC staining) and in situ hybridization (using both non- and isotopically-labeled cRNA probes) histochemical techniques, as well as assist in the optimization of procedures for specific applications; 4) Assist in the experimental design and interpretation of all histochemical experiments; and 5) Provide image analysis facilities and assistance with anatomical and quantitative analysis of histochemical experiments.

组织化学核心的目标是提供技术专长， 帮助实验设计，帮助解剖图像分析的帮助， 和现代组织化学方法中的培训机会。 组织化学方法是项目1-4的重要组成部分 计划项目。 核心实验室将提供设施和技术 在动物的组织学和组织化学加工方面的帮助 组织并避免购买昂贵的设备 每个实验室的这些程序。 核心还将提供 麻烦射击组织化学实验的必要专业知识，作为 很好地协助结果分析和定量。 经过 集中组织加工和协调实验 每个实验室使用的协议将有助于进步 达到每个项目的既定目标，并增强 在项目之间相互关联的结果。 Chinkers博士（项目1）将 利用组织化学核心评估 下丘脑中的亚钠肽，研究其可能的共同 CRF神经元中的表达，并定义 这些肽在下丘脑和垂体中的受体。 Low博士 （项目2）将需要免费访问核心设施来表征 下丘脑关键方面的发展和成人组织 缺乏β-的转基因小鼠中HPA轴的垂体成分 内啡肽。 托马斯博士（项目3）提出了需要的研究 访问从 用于免疫组织化学和原位杂交的转基因动物。 Cone博士（项目4）将需要广泛使用核心设施 研究新型黑素性受体受体的表达模式 成人和发展正常和转基因动物的大脑。 支持 在这些项目中，组织化学核心将：1）提供设备， 供应和技术援助以获取冷冻组织 使用低温恒温器和滑动微失位的切片； 2）储存冻结 组织样品（-ct）和滑动式部分，直到可以是 处理组织化学； 3）提供详细的协议 免疫组织化学（双荧光和ABC染色）和 原位杂交（使用非同位标记的CRNA探针） 组织化学技术，并有助于优化 特定应用程序的程序； 4）协助实验 所有组织化学实验的设计和解释； 5） 提供图像分析设施和解剖学和协助 组织化学实验的定量分析。