INTESTINAL AND PLACENTAL FC-RECEPTORS FOR IMMUNOGLOBULIN

肠和胎盘免疫球蛋白 FC 受体

• 批准号: 2200569
• 负责人: NEIL E SIMISTER
• 金额: $ 12.96万
• 依托单位: BRANDEIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-02-01 至 1995-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2200569
• 关键词: antibody receptor; biological signal transduction; chemical binding; corticosterone; developmental genetics; gastrointestinal epithelium; gene expression; gene rearrangement; genetic manipulation; genetic promoter element; genetic regulation; glucocorticoids; human fetus tissue; human tissue; immunoglobulin G; laboratory mouse; laboratory rabbit; laboratory rat; molecular cloning; newborn animals; nucleic acid sequence; placenta; placental transfer; polymerase chain reaction; protein structure function; protein transport; site directed mutagenesis; thyroxine; tissue /cell culture

The objective of the proposed research is to describe in molecular terms how immunoglobulins are transported from a pregnant or nursing mammal to her unborn or newborn offspring. The acquisition of maternal antibodies is essential for immunologic defense, although autoantibodies and immune complexes of live pathogens are potentially harmful to the neonate. We have two major goals. The first is to elucidate the molecular mechanism of intestinal IgG uptake in the suckling rat by an Fc receptor called FcRn (for neonate). This system will be used as a model for intracellular protein trafficking and specifically for the transmission of IgG across the human placenta. To identify and characterized charcterize the Fc receptor(s) that mediate placental transport of IgG is our second goal. Our specific aims are to: 1. Develop a monolayer cell culture model for transcytosis of IgG by FcRn. 2. Relate the structure of FcRn to its functions by measuring the properties of modified forms of the receptor in the cell culture model. 3. Study how the gene(s) that code for FcRn is (are) organized. 4. Study how the production of FcRn is regulated during development. 5. Identify and characterize by molecular cloning FcRs that transport IgG across human placenta.

拟议的研究的目的是用分子术语描述 如何将免疫球蛋白从怀孕或护理哺乳动物传输到 她的未出生或新生后代。孕产妇抗体的获取是 免疫防御至关重要，尽管自身抗体和免疫 活病原体的复合物可能对新生儿有害。我们 有两个主要目标。首先是阐明分子机制 通过称为FCRN的FC受体在哺乳大鼠中肠道IgG摄取 （对于新生儿）。该系统将用作细胞内的模型 蛋白质运输，专门用于IgG跨越 人体胎盘。识别和表征充电FC 介导IgG胎盘运输的受体是我们的第二个目标。 我们的具体目的是： 1。开发一种单层细胞培养模型，用于通过FCRN对IgG的转胞细胞增多症。 2。通过测量FCRN的结构与其功能相关联 细胞培养模型中受体的修饰形式的特性。 3。研究FCRN代码的基因是如何组织的。 4。研究在开发过程中如何调节FCRN的产生。 5。通过传输IgG的分子克隆FCR来识别和特征 遍布人体胎盘。