CORE--PATHOLOGY

核心--病理学

• 批准号: 5213252
• 负责人: BARBARA O MEYRICK
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5213252
• 关键词: adult respiratory distress syndrome; biomedical facility; electron microscopy; immunocytochemistry; light microscopy; lung injury; morphology; pathology

Core D will provide the techniques and morphologic expertise for the studies proposed in this SCOR Program. Services include the personnel, supplies and facilities for performing light and electron microscopy, immunohistochemistry on tissue sections and bronchoalveolar lavage cells, autoradiography and in situ hybridization. Quantitative techniques will be applied tumor necrosis factor-a (TNF-a), interleukin-8, endothelin-1 (ET-1) and PGH synthase will be examined in BAL cells from patients with the Adult Respiratory Distress Syndrome (ARDS). In situ hybridization with probes to each peptide will also be used to demonstrate alterations each of the genes. Such data will indicate whether TNF antibodies and glutathione ethyl esters alter regulation of these peptides. In addition, we will provide routine histological examination of all available autopsy lungs and lung biopsy tissue from these patients. This will allow correlations between the structural changes in our animal models and patients with ARDS. In we will examine the morphological alterations in the lung following administration of TNF-a, PGH synthase and ET-1 in the presence and absence of a TNF-a antibody, a neutrophil adhesion blocker, NPC15669, and the glutathione ethyl ester, GSE. These data will allow correlation between structural, functional and biochemical changes and provide insights into the efficacy of each "protective" agent. We will use in situ hybridization to localize xanthine oxidase mRNA in ling and liver of sheep during endotoxemia. In project V. we will examine lung, liver and kidney tissues for evidence of injury following administration of various forms of liposomes, examination of DNA/liposome complexes by scanning microscopy, localization of liposomes in organs using a fluorescent marker (5,6- carboxyfluorescein), localization of cDNA's in tissue by in situ hybridization, visualization of reporter genes such as chloramphenicol acetyltransferase (CAT) and beta-galactosamine, as well as localization of PGH synthase by immunohistochemical techniques. The techniques and interpretative skills of this Core are crucial to the successful completion of this SCOR application.

核心D将提供技术和形态专业知识 在此SCOR计划中提出的研究。 服务包括人员， 执行光和电子显微镜的供应和设施， 组织切片和支气管肺泡灌洗细胞的免疫组织化学， 放射自显影和原位杂交。 定量技术将 应用肿瘤坏死因子A（TNF-A），白介素8，内皮素-1 （ET-1）和PGH合酶将在来自患者的BAL细胞中检查 成人呼吸窘迫综合征（ARDS）。 原位杂交 对于每个肽的探针，也将用于演示改变 每个基因。 这样的数据将指示TNF抗体是否和 谷胱甘肽乙酯改变了这些肽的调节。 在 此外，我们将提供所有的常规组织学检查 这些患者的可用尸检和肺活检组织。 这 将允许我们动物的结构变化之间的相关性 模型和ARDS患者。 我们将检查形态学 施用TNF-A，PGH合酶后肺的改变 ET-1在存在和不存在TNF-A抗体的情况下，中性粒细胞 NPC15669的粘附阻滞剂和GSE的谷胱甘肽乙酯。 这些 数据将允许结构，功能和 生化变化并提供对每种功效的效力的见解 “保护性”代理。 我们将使用原位杂交来本地化 内毒素血症期间的绵羊和绵羊肝脏中的黄嘌呤氧化酶mRNA。 在 项目V.我们将检查肺，肝脏和肾脏组织以获取证据 施用各种形式的脂质体后的受伤， 通过扫描显微镜检查DNA/脂质体复合物， 使用荧光标记物在器官中的定位（5,6-- 羧基氟菌素），cDNA的定位在组织中的原位 杂交，可视化报告基因，例如氯霉素 乙酰转移酶（CAT）和β-半乳糖胺以及定位 通过免疫组织化学技术的PGH合酶。 技术和 该核心的解释技能对成功至关重要 完成此SCOR应用程序。