ENZYME THERAPY AND EXPERIMENTAL MAMMARY METASTASIS

酶疗法和实验性乳腺转移

基本信息

批准号：
2284866
负责人：
LEONARD A COHEN
金额：
$ 3万
依托单位：
INSTITUTE FOR CANCER PREVENTION
依托单位国家：
美国
项目类别：
财政年份：
1994
资助国家：
美国
起止时间：
1994-09-30 至 1995-09-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2284866
关键词：
alternative medicine antineoplastics breast neoplasms disease /disorder model enzyme therapy female laboratory rat metastasis neoplasm /cancer chemotherapy neoplasm /cancer nutrition therapy neoplastic cell nonhuman therapy evaluation nutrition related tag oral administration pancreas enzyme postmenopause statistics /biometry

项目摘要

DESCRIPTION: (Investigator's Abstract); In alternative medicine, pancreatic enzymes have a long and controversial history, dating back to the nineteenth century. The primary objective of this proposal is to determine whether enzyme therapy, is an effective means of inhibiting the growth of a primary mammary tumor and its dissemination to a distant organ.To this end, clone MTLn3 derived from the R-13762 metastasizing mammary tumor will be used as a model for human breast cancer metastasis. Tumor cells will be inoculated orthotopically, i.e. into the mammary fat pad, into two groups of F-344 female rats: (1) young virgins aged 6-9 weeks (premenopausal model) and (2) retired breeders aged 40-45 weeks (postmenopausal model). The two groups will be subdivided into four subgroups and a dehydrated porcine pancreatic preparation fed during the light cycle at 3.5, 2, 1 and 0 gms/day to 12 rats per group, respectively. After 40 days, all animals will be sacrificed and growth of the primary tumors and pulmonary metastases enumerated. The pancreative preparation, as well as dose and time of feeding are based, by extrapolation, on the clinical studies of Dr. N. Gonzalez who is a co-investigator on this proposal. The significance of this study is threefold.First, it involves experimental validation of an alternative medical procedure which, though used in the clinic, has not been tested by accepted scientific methods. Second, it provides a collaboration between a practitioner of alternative medicine and an experienced cancer researcher. Third, if successful, it will provide the basis for a clinical trial based on accepted scientific methods.

描述：（研究者的摘要）；在替代医学中，胰腺酶具有悠久而有争议的历史，可追溯到到19世纪。该提议的主要目的是确定酶治疗是否是抑制的有效手段原发性乳腺肿瘤的生长及其传播到远处器官。到此目的，克隆mtln3衍生自R-13762转移乳腺肿瘤将用作人类乳腺癌的模型转移。肿瘤细胞将原始接种，即进入乳腺脂肪垫分为两组F-344雌鼠：（1）处女6-9周（绝经前模型）和（2）退休的育种者年龄40-45周（绝经后模型）。两组将是细分为四个亚组和一个脱水的猪胰腺在3.5、2、2、1和0 gms/天到12的光周期中喂养的准备工作每组大鼠分别。 40天后，所有动物都将原发性肿瘤和肺转移的生长枚举。胰腺准备，剂量和时间喂养是通过外推基于N. N.的临床研究的。冈萨雷斯（Gonzalez）是该提案的共同投资者。意义这项研究的三倍。首先，它涉及实验验证替代医疗程序，尽管在诊所中使用了尚未通过公认的科学方法进行测试。其次，它提供了替代医学从业者与经验丰富的癌症研究员。第三，如果成功，它将提供基于公认的科学方法的临床试验的基础。