EUKARYOTIC DNA J PROTEINS THAT REGULATE HSP70 ACTION

调节 HSP70 作用的真核 DNA J 蛋白

• 批准号: 2184792
• 负责人: Pamela A Silver
• 金额: $ 19.78万
• 依托单位: DANA-FARBER CANCER INST
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-05-01 至 1996-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2184792
• 关键词: DNA binding protein; SDS polyacrylamide gel electrophoresis; Saccharomyces cerevisiae; adenosinetriphosphatase; endonuclease; enzyme activity; fungal genetics; immunoelectron microscopy; immunoprecipitation; intracellular transport; laboratory rabbit; low angle X ray diffraction analysis; nuclear magnetic resonance spectroscopy; nucleic acid hybridization; polymerase chain reaction; protein folding; protein structure function; protein transport; radionuclides; stress proteins

Protein folding has often been assumed to be a spontaneous process, a classic example of biological "self-assembly". However, it has recently become apparent that protein folding and assembly of multimeric protein complexes can be catalyzed by other proteins. the highly conserved heat shock proteins (HSPs) comprise one class of these so-called "chaperonins". The long term goal of the proposed research is to determine the mechanism of action of the recently discovered DnaJ family of eukaryotic heat shock protein homologues. In bacteria, DnaJ interacts with and modulates the activities of DnaK, the HSP70 cognate. The proposed experiments are designed with this paradigm in mind. A combination of yeast genetics, molecular biological, and biochemical approaches will be employed. The specific aims are: 1) to determine the role of Scjs in protein translocation into organelles; 2) to define the interaction between Scjs and HSP70s; 3) to test the hypothesis that each eukaryotic HSP70 will have one or more like DnaJ-like partners; and 4) to dissect the proposed domain organization of Scjs.

蛋白质折叠通常被认为是自发过程 生物学“自组装”的经典例子。 但是，最近有 显而易见的是，多聚体蛋白的蛋白质折叠和组装 复合物可以由其他蛋白质催化。 高度保守的热量 休克蛋白（HSP）包括其中一类所谓的“伴侣蛋白”。 拟议研究的长期目标是确定机制 最近发现的真核热休克家族的作用 蛋白质同源物。 在细菌中，DNAJ与并调节 DNAK的活动，HSP70同源。 提出的实验是 考虑到这种范式。 酵母遗传学的结合， 将采用分子生物学和生化方法。 这 具体目的是：1）确定SCJ在蛋白质中的作用 转运为细胞器； 2）定义SCJ之间的相互作用 和HSP70； 3）测试每个真核生物HSP70都将具有的假设 一个或多个类似DNAJ的合作伙伴； 4）剖析提出的域 SCJS的组织。