FLOW INJECTION CYTOMETRY IN ANALYTICAL BIOLOGY

分析生物学中的流式注射细胞术

• 批准号: 3304662
• 负责人: JAROMIR RUZICKA
• 金额: $ 16.83万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-01-01 至 1993-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3304662
• 关键词: biomedical automation; cytology; flow cytometry; fluid flow; fluorimetry; magnetism; method development; stainings

The purpose of this research is to develop automated sample and solution handling procedures in the analysis of cells, cell organelles and chemical substances of relevance in that area. In this work the principles and methodology of flow injection analysis (FIA) will be adopted and further developed for that purpose. For detection, flow fluorimetry and flow cytometry will be used. The developed technology will allow kinetic analysis of faster cell reactions than is possible with present methodology. Further, using FIA, accurate temporal, stoichiometric and temperature control in reagent-cell and agonist/antagonist-cell reactions becomes available. A third area of research is automated separation and analysis of cells and cell related substances. This field will be pursued using in-line filters in combination with flow direction reversals as well as antibody labeled magnetic particles controlled by electromagnets. The precise timing of experimental events such as valve switching, setting flow speeds, flow stop/go, flow reversals, electromagnetic capturing and so on, will be done using computer (PC) controlled instruments.

这项研究的目的是开发自动样本和解决方案 在分析细胞，细胞器和化学的分析中处理程序 相关物质在该领域。 在这项工作中，原则和 流量注射分析方法（FIA）将被采用，并进一步 为此目的开发。 为了检测，流动荧光和流动 将使用细胞仪。 开发的技术将允许动力学 分析比现在可能更快的细胞反应 方法论。 此外，使用FIA，准确的时间，化学计量和 试剂细胞和激动剂/拮抗细胞反应中的温度控制 可用。 研究的第三个领域是自动分离， 细胞和细胞相关物质的分析。 该领域将被追捕 在线过滤器也将流动方向逆转结合使用 作为由电磁体控制的抗体标记的磁颗粒。 这 实验事件的精确时机，例如阀门切换，设置流量 速度，流量/GO，流动逆转，电磁捕获等等，依此类推， 将使用计算机（PC）受控仪器完成。