PHYSIOLOGICAL ROLES OF NEUROENDOCRINE PEPTIDES IN THE REGULATION OF REPRODUCTION

神经内分泌肽在生殖调节中的生理作用

• 批准号: 3735277
• 负责人: CATHERINE RIVIER
• 金额: --
• 依托单位: SALK INSTITUTE FOR BIOLOGICAL STUDIES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3735277
• 关键词: adrenocorticotropic hormone; catecholamines; corticosteroids; endogenous opioid; estrus; gender difference; gene expression; gonadotropin releasing factor; hormone regulation /control mechanism; hypothalamic pituitary axis; inhibin; interleukin 1; interleukin 6; laboratory rat; luteinizing hormone; median eminence; messenger RNA; neuroendocrine system; neutralizing antibody; peptide hormone; pituitary gonadal axis; prostaglandins; reproduction; rheumatoid arthritis; testosterone

Activin and inhibin are gonadal proteins that alter both pituitary and ovarian function. The work presented in the present proposal represents an extension of our previous studies of the effects of, and the role played by these proteins. We have observed that the peripheral administration of activin increased GnRH mRNA levels in the hypothalamus. Under Specific Aim 1, we will determine whether inhibin also alters GnRH gene expression; whether the effect of activin (and possibly inhibin) on GnRH transcripts are mediated by sex steroids; whether changes in brain GnRH levels are accompanied by altered release of the peptide from the median eminence; and whether endogenous opiates play a role in these effects. We will also investigate the role of activin during the estrous cycle and during the early increase in gonadotropin levels caused by gonadectomy, by blocking activin with specific neutralizing antibodies of the ligand binding domain. These experiments will address as yet unexplored aspects of the role and effects of inhibin/activin.

激活素和抑制素是改变垂体和 卵巢功能。本提案中介绍的工作代表 我们先前关于影响和作用的研究的扩展 由这些蛋白质发挥作用。我们已经观察到外围 启动素的给药增加了下丘脑中的GnRH mRNA水平。 在特定目标1下，我们将确定抑制素是否也会改变GNRH 基因表达；激活素（以及可能抑制素）对 GNRH转录本是由性类固醇介导的。大脑的变化是否 GNRH水平伴随着改变肽从 中位数;以及内源性阿片类药物是否在这些中发挥作用 效果。我们还将研究激活素在发情过程中的作用 周期以及在早期增加的促性腺激素水平 通过特异性中和抗体阻断激活素的性腺切除术 配体结合结构域。这些实验还将解决 抑制素/活化素的作用和作用的未开发方面。