GENETIC ANALYSIS OF NEMATODE DEVELOPMENT

线虫发育的遗传分析

• 批准号: 2177271
• 负责人: Victor Ambros
• 金额: $ 27.13万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1996-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2177271
• 关键词: Caenorhabditis elegans; alleles; cell cell interaction; cell cycle; cell differentiation; developmental genetics; electron microscopy; electroporation; fluorescence microscopy; gene complementation; gene expression; gene interaction; gene mutation; genetic manipulation; genetic mapping; genetic regulatory element; genetic transcription; molecular cloning; mutagen testing; northern blottings; tissue mosaicism; transcription factor; video recording system; western blottings

The broad goal of this work is to understand the genetic and molecular mechanisms of temporal control of cell division and differentiation using the nematode C. elegans as a model. Genetic and molecular analysis will be performed to determine how the regulatory pathway of heterochronic genes controls the temporally coordinated expression of diverse developmental events in C. elegans larvae. Emphasis will be placed on understanding how certain genes control the timing of events in diverse cell types, and how other genes act to specify the execution of particular events. Genetic screens will be employed to identify new genes in this pathway. Key genes will be analysed molecularly to determine the structure and expression of their products, and how they interact with the products of other heterochronic genes. Mosaic analysis will be used to assess the role of these genes in cell-cell signalling processes that coordinate developmental programs in separate cell lineages. Emphasis will be placed on understanding the molecular mechanism of how lin-14, a gene that plays a central role in the control of diverse larval cell lineages, is regulated by the novel regulatory molecule encoded by lin-4. Also a major effort will be focused on understanding how the transcription factor encoded by lin-29 is developmentally regulated, and how it controls the transcription of adult-specific and larval-specific genes. Generally speaking, the C. elegans heterochronic genes offer an opportunity to study the genetic and molecular mechanisms controlling cell division and differentiation, processes central to all multicellular development. More specifically, the fact that the heterochronic genes affect developmental timing provides a unique system in which to examine in detail the regulatory mechanisms underlying temporal control.

这项工作的广泛目标是了解遗传和分子 使用细胞分裂的时间控制和分化的机制 线虫秀丽隐杆线虫作为模型。 遗传和分子分析将 执行以确定杂音的调节途径如何 基因控制着各种各样的时间协调表达 秀丽隐杆线虫幼虫的发展事件。 重点将放在 了解某些基因如何控制多样化事件的时机 细胞类型，以及其他基因如何指定执行 特殊事件。 遗传屏幕将被用来识别新的 该途径中的基因。 关键基因将分子分析至 确定其产品的结构和表达以及如何 与其他异缘基因的产物相互作用。 马赛克分析 将用于评估这些基因在细胞细胞信号传导中的作用 在单独的单元格中协调开发程序的过程 血统。 重点将放在理解分子 Lin-14的机制，一种在控制中起着核心作用的基因 多种幼虫细胞谱系的调节性调节 分子由LIN-4编码。 同样将重点放在 了解LIN-29编码的转录因子是如何 开发监管，以及它如何控制的转录 成人特异性和幼虫特异性基因。 一般来说，C。 秀丽隐杆线杂化基因提供了研究遗传和 控制细胞分裂和分化的分子机制， 所有多细胞开发的核心过程。 更具体地说， 异缘基因影响发育时机的事实 提供了一个独特的系统，可以在其中详细检查监管 时间控制的基础机制。