AFFERENT MODULATION OF RESPIRATORY PATTERN IN VITRO

体外呼吸模式的传入调节

• 批准号: 2214244
• 负责人: NICHOLAS M MELLEN
• 金额: $ 2.37万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-10-04 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2214244
• 关键词: AFFERENT; MODULATION; RESPIRATORY; PATTERN; VITRO

The isolated neonate rat brainstem/spinal cord preparation will be used to investigate the interaction between neural populations mediating the generation of respiratory rhythm and afferent inputs that reset this rhythm. In this preparation, networks generating respiratory rhythm are fully functional, and their cellular and synaptic properties are readily accessible. Endogenous bursters (cells whose membrane potentials oscillate in the absence of synaptic input) have been identified in this preparation. In some models of respiratory rhythmogenesis, endogenous bursters determine respiratory frequency by their bursting frequency, which is known to be a function of their resting membrane potentials. Preliminary studies show that respiratory rhythm is reset by electrical stimulation to the vagus nerve in this preparation. The effect of stimulation is a function of when it occurs in the respiratory cycle: inspiration is shortened when the stimulus is applied during inspiration, expiration is lengthened when the stimulus is applied early in expiration, and shortened when the stimulus is applied late in expiration. If endogenous bursters determine respiratory frequency, then stimulus-induced resetting of respiratory rhythm must converge on these cells. Monitoring endogenous bursters during stimulus-induced resetting of respiratory rhythm will test this hypothesis, and differentiate between alternative models of the functional circuitry for respiratory rhythmogenesis. Characterizing the cellular and synaptic mechanisms mediating the interaction of centrally generated rhythm and afferent feedback may shed light on the neural basis of respiratory pathologies which arise out of the inappropriate coupling of centrally generated rhythm and sensory feedback. In this preparation the neural basis for such pathologies can be investigated, and novel therapeutic interventions developed.

将使用分离的新生大鼠脑干/脊髓制剂 研究介导神经群体之间的相互作用 呼吸节律的产生和重置此的传入输入 韵律。在此准备中，产生呼吸节律的网络是 功能齐全，并且它们的细胞和突触特性很容易 可以访问。内源性爆发细胞（膜电位 在没有突触输入的情况下振荡）已在此确定 准备。在一些呼吸节律发生模型中，内源性 爆发器通过其爆发频率确定呼吸频率， 已知这是其静息膜电位的函数。 初步研究表明，呼吸节律可通过电重置 该制剂刺激迷走神经。的效果 刺激是呼吸周期中发生时间的函数： 当吸气期间施加刺激时，吸气时间会缩短， 当早期施加刺激时，到期时间会延长 到期，并在刺激施加较晚时缩短 到期。如果内源性爆发决定呼吸频率，那么 刺激引起的呼吸节律重置必须集中于这些 细胞。在刺激诱导的重置过程中监测内源性爆发 呼吸节律的研究将检验这一假设，并区分 呼吸功能电路的替代模型之间 节律发生。 表征介导的细胞和突触机制 中央生成的节奏和传入反馈的相互作用可能会消失 浅谈呼吸系统疾病的神经基础 中枢产生的节奏和感觉的不适当耦合 反馈。在这种准备中，此类病理的神经基础可以 进行研究，并开发新的治疗干预措施。