AFFERENT MODULATION OF RESPIRATORY PATTERN IN VITRO

体外呼吸模式的传入调节

• 批准号: 2214244
• 负责人: NICHOLAS M MELLEN
• 金额: $ 2.37万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-10-04 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2214244
• 关键词: AFFERENT; MODULATION; RESPIRATORY; PATTERN; VITRO

The isolated neonate rat brainstem/spinal cord preparation will be used to investigate the interaction between neural populations mediating the generation of respiratory rhythm and afferent inputs that reset this rhythm. In this preparation, networks generating respiratory rhythm are fully functional, and their cellular and synaptic properties are readily accessible. Endogenous bursters (cells whose membrane potentials oscillate in the absence of synaptic input) have been identified in this preparation. In some models of respiratory rhythmogenesis, endogenous bursters determine respiratory frequency by their bursting frequency, which is known to be a function of their resting membrane potentials. Preliminary studies show that respiratory rhythm is reset by electrical stimulation to the vagus nerve in this preparation. The effect of stimulation is a function of when it occurs in the respiratory cycle: inspiration is shortened when the stimulus is applied during inspiration, expiration is lengthened when the stimulus is applied early in expiration, and shortened when the stimulus is applied late in expiration. If endogenous bursters determine respiratory frequency, then stimulus-induced resetting of respiratory rhythm must converge on these cells. Monitoring endogenous bursters during stimulus-induced resetting of respiratory rhythm will test this hypothesis, and differentiate between alternative models of the functional circuitry for respiratory rhythmogenesis. Characterizing the cellular and synaptic mechanisms mediating the interaction of centrally generated rhythm and afferent feedback may shed light on the neural basis of respiratory pathologies which arise out of the inappropriate coupling of centrally generated rhythm and sensory feedback. In this preparation the neural basis for such pathologies can be investigated, and novel therapeutic interventions developed.

将使用孤立的新生儿大鼠脑干/脊髓制备 调查介导的神经种群之间的相互作用 生成呼吸节奏和传入输入的重置 韵律。在此准备中，产生呼吸节奏的网络是 功能齐全，其细胞和突触特性很容易 可访问。内源囊泡（其膜电位的细胞 在没有突触输入的情况下，振动液已在此确定 准备。在某些呼吸节奏发生的模型中，内源性 毛毛器通过其爆发频率确定呼吸频率， 已知这是其静止膜电位的函数。 初步研究表明，呼吸节奏是通过电气复位的 在这种制剂中刺激迷走神经。效果 刺激是何时发生在呼吸周期中的函数： 当灵感期间应用刺激时，灵感会缩短 早期应用刺激时，到期会延长 到期，当刺激在后期应用时缩短 到期。如果内源囊泡确定呼吸频率，则 刺激引起的呼吸节奏重置必须汇合这些 细胞。在刺激引起的重置期间监测内源囊泡 呼吸节奏将检验这一假设，并区分 在呼吸的功能电路的替代模型之间 节律发生。 表征介导的细胞和突触机制 中心产生的节奏和传入反馈的相互作用可能会散发出来 基于呼吸道病的神经基础的光 中心产生的节奏和感觉的不适当耦合 反馈。在这种准备中，这种病理的神经基础可以 进行研究，并制定了新的治疗干预措施。