MOLECULAR ANALYSIS OF SEXUAL DEVELOPMENT IN NEUROSPORA

神经孢子虫性发育的分子分析

• 批准号: 2184779
• 负责人: MARY A NELSON
• 金额: $ 11.38万
• 依托单位: UNIVERSITY OF NEW MEXICO
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-05-01 至 1997-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2184779
• 关键词: DNA footprinting; Neurospora; biochemical evolution; confocal scanning microscopy; fungal genetics; gene expression; gene interaction; gene mutation; genetic mapping; genetic regulatory element; genetic transcription; molecular cloning; northern blottings; nucleic acid sequence; phenotype; protein structure function; sex differentiation; southern blotting

The long term objective of this research is to characterize the sexual development of the filamentous fungi at the molecular level. Although these organisms are of vast economic and social importance, very little is known of their development. The much-studied bread mold Neurospora crassa will be used as the model organism. Neurospora undergoes a complex process of sexual differentiation; a number of the regulatory and target (mating-specific) genes that are involved in this process have already been identified. The structures and functions of these genes will be examined, as will the control of their expression and the interactions between their gene products. The roles of the mating-specific genes in sexual development will be determined using a reverse-genetic approach, and the phenotypes observed when these genes are dysfunctional will be characterized microscopically. The evolutionary conservation of the mating-specific genes will be examined. The factors that cause these genes to be expressed specifically during sexual development will be studied; in preliminary experiments, three control pathways were identified. The interactions between these control pathways will be examined, and a search for other elements involved in the control of gene expression during sexual development will be executed. The structures (nucleotide sequences) of a regular gene (fmf-1) and selected mating-specific target genes will be determined, and the properties of their encoded products examined. An in vitro analysis of the regulatory elements recognized during sexual development will be carried out using mobility shift assays and DNA footprinting experiments; this analysis will serve to identify important control elements and further define the roles played by the regulatory gene products. Finally, the hierarchy of events required for normal sexual development in Neurospora will be examined, by determining the effects of selected processes on the overall sexual developmental pathway. Some filamentous fungi are exploited for the production of useful compounds, but others cause devastating diseases. Basic information about the control of their life processes will allow judicious use of the beneficial fungi, and will also further efforts to control the pathogens.

这项研究的长期目标是表征性 分子水平的丝状真菌的发展。 虽然 这些生物具有巨大的经济和社会重要性，很少 知道他们的发展。 研究备受研究的面包模具神经孢菌 Crassa将用作模型生物。 Neurospora经历a 性别差异的复杂过程；许多监管和 参与此过程的靶标（特定于配合的）基因 已经确定了。 这些基因的结构和功能 将被检查，对其表达的控制和 它们的基因产品之间的相互作用。 交配基因在性发展中的作用将是 使用反向遗传学方法确定，并观察到表型 当这些基因功能障碍时，将通过显微镜表征。 配合特异性基因的进化保护将是 检查。 导致这些基因表达的因素 特别是在性发展期间进行研究；在初步 实验，确定了三个控制途径。 交互 将检查这些控制途径之间，并搜索其他 性别控制基因表达涉及的元素 开发将被执行。 A的结构（核苷酸序列） 常规基因（FMF-1）和选定的特定靶靶基因将是 确定的，并检查了其编码产品的特性。 一个 对性识别的调节元素的体外分析 开发将使用移动性转移分析和DNA进行 足迹实验；该分析将有助于确定重要 控制元素并进一步定义监管的角色 基因产品。 最后，正常所需的事件的层次结构 通过确定 选定过程对整体性发展的影响 路径。 一些丝状真菌被利用用于生产有用的 化合物，但其他人会引起毁灭性疾病。 基本信息 关于他们的生活过程的控制将允许明智地使用 有益的真菌，也将进一步努力控制病原体。