MEASURE OF RECEPTOR AND POST RECEPTOR ACTIVITY

受体和受体后活性的测量

• 批准号: 2162689
• 负责人: DONALD C. HOOD
• 金额: $ 19.27万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-05-01 至 1999-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2162689
• 关键词: brain electrical activity; cone cell; electroretinography; human subject; mathematical model; model design /development; retina disorder; rod cell; visual phototransduction; visual stimulus

The electroretinogram, ERG, is a valuable noninvasive measure of retinal activity. It is commonly used for diagnosing and following the course of retinal diseases. By building upon discoveries by the retinal physiologist, the usefulness of the ERG, the summed activity of the retinal cells, can now be extended. During the current grant period we have provided strong evidence that the leading edge of the human rod ERG is quantitatively described by a recent model of rod phototransduction. This portion of the ERG is the sum of the activity of individual rod receptors. These findings indicate that the a-wave can be employed to answer fundamental questions about the transduction process of normal and abnormal human rods. These questions will be addressed by fitting models of transduction to ERGs from patients with a variety of retinal disorders. During the current grant period, we have also provide evidence that the human cone receptors can be studied in a similar way. One of the aims of the proposed research is to measure human cone receptor activity in vivo. and to develop techniques for testing hypotheses for disease related changes in cone function. This will be accomplished by fitting models of transduction to the cone isolated a-waves of normals, dichromats, and patients with different retinal diseases. Finally, by building upon the models of the receptor, models of the ERG that include the activity of the inner nuclear layer will be developed and applied to ERGs recorded from individuals with and without retinal problems.

电视图，ERG是视网膜的有价值的无创测量 活动。它通常用于诊断和遵循 视网膜疾病。通过在视网膜发现的基础上建立 生理学家，ERG的实用性，总的活动 现在可以扩展视网膜细胞。在当前的赠款期内我们 提供了有力的证据表明人杆ERG的前缘 通过杆光转导的最新模型进行定量描述。 ERG的这一部分是单个杆活动的总和 受体。这些发现表明可以使用A波 回答有关正常转导过程的基本问题 异常人杆。这些问题将通过拟合模型来解决 来自各种视网膜疾病患者的ERG转导。 在当前赠款期间，我们还提供了证据表明 可以以类似的方式研究人锥体受体。目的之一 拟议的研究是在体内测量人锥受体活性。 并开发用于测试与疾病相关的假设的技术 锥功能的变化。这将通过拟合的模型来完成 转导向锥体分离的正常的A波，Dichromats和 视网膜疾病不同的患者。最后，通过建立 受体的模型，ERG的模型，包括 内部核层将开发并应用于从 有和没有视网膜问题的人。