MEASURE OF RECEPTOR AND POST RECEPTOR ACTIVITY

受体和受体后活性的测量

• 批准号: 2162689
• 负责人: DONALD C. HOOD
• 金额: $ 19.27万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-05-01 至 1999-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2162689
• 关键词: brain electrical activity; cone cell; electroretinography; human subject; mathematical model; model design /development; retina disorder; rod cell; visual phototransduction; visual stimulus

The electroretinogram, ERG, is a valuable noninvasive measure of retinal activity. It is commonly used for diagnosing and following the course of retinal diseases. By building upon discoveries by the retinal physiologist, the usefulness of the ERG, the summed activity of the retinal cells, can now be extended. During the current grant period we have provided strong evidence that the leading edge of the human rod ERG is quantitatively described by a recent model of rod phototransduction. This portion of the ERG is the sum of the activity of individual rod receptors. These findings indicate that the a-wave can be employed to answer fundamental questions about the transduction process of normal and abnormal human rods. These questions will be addressed by fitting models of transduction to ERGs from patients with a variety of retinal disorders. During the current grant period, we have also provide evidence that the human cone receptors can be studied in a similar way. One of the aims of the proposed research is to measure human cone receptor activity in vivo. and to develop techniques for testing hypotheses for disease related changes in cone function. This will be accomplished by fitting models of transduction to the cone isolated a-waves of normals, dichromats, and patients with different retinal diseases. Finally, by building upon the models of the receptor, models of the ERG that include the activity of the inner nuclear layer will be developed and applied to ERGs recorded from individuals with and without retinal problems.

视网膜电图 (ERG) 是一种有价值的视网膜无创测量方法 活动。它通常用于诊断和跟踪病程 视网膜疾病。以视网膜的发现为基础 生理学家、ERG 的有用性、 视网膜细胞，现在可以延伸。在当前的资助期内，我们 提供了强有力的证据表明人体杆ERG的前沿 最近的杆状光转导模型定量地描述了这一点。 ERG 的这一部分是各个杆的活动的总和 受体。这些发现表明 a 波可用于 回答有关正常和正常细胞转导过程的基本问题 异常的人体杆。这些问题将通过拟合模型来解决 患有各种视网膜疾病的患者转导为 ERG。 在当前的资助期内，我们还提供了证据表明 人类视锥受体可以用类似的方式研究。的目标之一 拟议的研究是测量人体视锥细胞受体的体内活性。 并开发测试疾病相关假设的技术 锥体功能的变化。这将通过拟合模型来完成 转导到锥体隔离的法线、二色视和 患有不同视网膜疾病的患者。最后，通过建立在 受体模型、ERG 模型，包括 内核层将被开发并应用于记录的 ERG 有或没有视网膜问题的人。