EXPERIMENTAL ACUTE ANTERIOR UVEITIS

实验性急性前葡萄膜炎

基本信息

批准号：
2164473
负责人：
HENRY Jerrold KAPLAN
金额：
$ 20.1万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1994
资助国家：
美国
起止时间：
1994-04-01 至 1999-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2164473
关键词：
MHC class II antigen T cell receptor acute disease /disorder anergy antigens autoimmune disorder cell adhesion molecules cell population study disease /disorder model hybridomas immunopathology immunotherapy inflammation iridocyclitis laboratory mouse laboratory rabbit laboratory rat major histocompatibility complex melanins model design /development molecular cloning monoclonal antibody oxidoreductase inhibitor passive immunization

MHC class II antigen T cell receptor acute disease /disorder anergy antigens autoimmune disorder cell adhesion molecules cell population study disease /disorder model hybridomas immunopathology immunotherapy inflammation iridocyclitis laboratory mouse laboratory rabbit laboratory rat major histocompatibility complex melanins model design /development molecular cloning monoclonal antibody oxidoreductase inhibitor passive immunization

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项目摘要

The most frequent form of human intraocular inflammation is acute anterior uveitis (AAU) of unknown etiology. The recurrent nature of the disease can lead to permanent visual loss from the secondary complications of cystoid macular edema, posterior subcapsular cataract or glaucoma. Consequently, it is a major cause of visual disability in our society. Until recently, no experimental animal model of AAU existed. In the most widely studied model of autoimmune intraocular inflammation, experimental autoimmune uveitis (EAU) to various soluble retinal proteins, the retina and choroid are the foci of inflammation not the iris/ciliary body (CB) as in human AAU. We, as well as Brockhuyse and colleagues, have described a new experimental model in the Lewis rat which is induced by immunization with a bovine melanin associated protein derived from the iris/CB and which mimics human AAU. We refer to it as experimental acute anterior uveitis (BAAU). The studies in this proposal are designed to complete the immunologic characterization of the autoimmune model, to identify the pathogenic protein and its immunodominant epitopes, to explore the immunopathogenesis of the disease and to study the efficacy of various treatment approaches. The opportunity to directly study a clinically relevant model of intraocular inflammation is exciting and provides unique possibilities to understand and prevent a disease which is associated with significant morbidity and which can only be treated symptomatically, but not cured.

人眼炎症的最常见形式是急性未知病因的前葡萄膜炎（AAU）。复发性的性质该疾病可能导致次要的永久视觉丧失囊状黄斑水肿的并发症，后囊白内障或青光眼。因此，这是视觉的主要原因我们社会的残疾。直到最近，还没有AAU的实验动物模型。在最广泛研究的自身免疫性炎症模型，实验性自身免疫性葡萄膜炎（EAU）到各种可溶性视网膜蛋白质，视网膜和脉络膜是炎症的焦点虹膜/睫状体（CB）和人类AAU一样。我们以及 Brockhuyse及其同事描述了一种新的实验模型在用牛免疫诱导的刘易斯大鼠中黑色素相关的蛋白质源自虹膜/CB，其中模仿人AAU。我们将其称为实验性急性前部葡萄膜炎（Baau）。该提案中的研究旨在完成自身免疫模型的免疫特征，以识别致病蛋白及其免疫主流表位，以探索该疾病的免疫发病发生，并研究各种治疗方法。直接研究的机会眼内炎症的临床相关模型令人兴奋并提供了理解和防止的独特可能性疾病与明显的发病率有关只能对症状进行治疗，但不能治愈。