COMPARISON OF TWO TECHNIQUES FOR BONE LEAD MEASUREMENT

两种骨导测量技术的比较

• 批准号: 2155415
• 负责人: ANDREW C TODD
• 金额: $ 32.48万
• 依托单位: MOUNT SINAI SCHOOL OF MEDICINE OF CUNY
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-05-01 至 1998-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2155415
• 关键词: X ray; atomic absorption spectrometry; bone; chelation therapy; environmental toxicology; fluorescence polarization; human subject; human therapy evaluation; lead; measurement; method development; occupational hazard; radiation detector; radiation dosage; radiography

Two non-invasive radiation techniques have been developed to measure lead in bone; K-shell and L-shell X-Ray Fluorescence (XRF). Both provide quantitative data on bone-lead content. The two techniques have not undergone direct comparison. We hypothesize that the two technologies will provide different and complementary information on bone-lead burden, in that L XRF gives data on lead in superficial, mainly subperiosteal bone, whereas K XRF provides data on deep or whole bone-lead content. We hypothesize further that superficial bone-lead is more closely correlated with chelatable lead, the standard clinical indicator of lead accumulation, but that deep bone-lead is the better marker of cumulative absorption and hence of chronic toxicity. To test these hypotheses, we propose to construct a state-of-the-art L- shell XRF instrument and then conduct a study to compare and contrast the K and L XRF techniques. This study will be undertaken within the Heavy Metals core of the Mount Sinai Environmental Health Sciences Center. A polarized L XRF system will measurement system. Operating characteristic and limitations will be defined: precision; detection limit; than lead (particularly arsenic); and effective (radiation) dose. To determine whether the K and L technologies provide data on lead in two distinct compartments within the body lead burden, analyses will be conducted by intact was well as with the soft-tissue remove. Chemical analyses by atomic absorption spectrophotometry of deep and superficial bone samples obtained from the same sites in the same legs will provide additional experimental validation of the XRF techniques. To determine whether the two technologies are measuring compartments containing lead of differential accessibility, and an examination of the effects of chelation therapy will be performed. Both K and L XRF will be used to measure lead content of bone both before and after therapeutic chelation in a population of sixty construction workers occupationally exposed to lead. Data from this study will extend current understanding of the radiation technologies used to measure bone-lead content and will increase knowledge of the toxicokinetics of lead in bone.

已经开发了两种非侵入性辐射技术来测量铅 在骨头K壳和L壳X射线荧光（XRF）。 两者都提供 骨铅含量的定量数据。 这两种技术没有 经过直接比较。 我们假设这两种技术 将提供有关骨头负担的不同和互补的信息， 在该l XRF中，XRF提供了浅表，主要是层次的铅的数据 骨头，而K XRF提供了有关深或全骨铅含量的数据。 我们进一步假设浅骨铅更接近 与Chelatable Lead相关，这是铅的标准临床指标 积累，但深骨铅是累积的更好标志 吸收和慢性毒性。 为了检验这些假设，我们建议构建最先进的l- 壳XRF仪器，然后进行研究以比较和对比 K和L XRF技术。 这项研究将在沉重的 西奈山环境健康科学中心的金属核心。 一个 偏振XRF系统将测量系统。 操作特征 将定义限制：精度；检测极限；比铅 （特别是砷）；和有效（辐射）剂量。 确定 K和L技术是否提供了两个不同的铅数据 人体内部的隔室牵引负担，分析将由 完好无损的情况下，软组织删除也是如此。 化学分析 深层和浅表骨样品的原子吸收分光光度法 从同一腿上的同一站点获得的将提供额外 XRF技术的实验验证。 确定是否 两种技术正在测量包含铅的室 差异可访问性以及对 将进行螯合疗法。 K和L XRF都将用于 在治疗螯合之前和之后测量骨头的铅含量 在六十名建筑工人的职业中 带领。 这项研究的数据将扩展对辐射的当前理解 用于测量骨头含量的技术，将增加 了解骨头中铅的毒性动力学的知识。