GASTRIC ENTEROCHROMAFFIN LIKE CELL IN ULCER DISEASE

溃疡病中的胃肠嗜铬样细胞

• 批准号: 2146201
• 负责人: George Sachs
• 金额: $ 15.59万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-05-01 至 1998-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2146201
• 关键词: acidity /alkalinity; calcium flux; chromaffin cells; electrical potential; exocytosis; fluorescent dye /probe; gastric acid; gastric mucosa; genetic library; histamine release; hormone regulation /control mechanism; intracellular transport; laboratory rat; membrane channels; polymerase chain reaction; secretion; single cell analysis; tissue /cell culture

The gastric enterochromaffin-like (ECL) cell is the cell responsible for histamine release within the gastric fundic mucosa, deemed essential for activation of the H2 receptor on the parietal cell. It therefore plays a central role in regulation of acid secretion. It constitutes 1% of cell volume in fundic epithelium. It has been isolated and purified to 85% cell content from rat gastric mucosa, by a combination of elutriation, density gradient centrifugation and short term culture. Histamine release is stimulated by gastrin/CCK, carbachol and epinephrine and inhibited by somatostatin, H3 receptor agonist and CCK B antagonists. The mechanisms of stimulus secretion coupling in this cell are unknown. These will be investigated by image analysis of changes in intracellular calcium, chloride and pH, by cell attached and whole cell measurements and by defining the proteins involved in granule exocytosis by screening a cDNA library derived from the purified ECL-cell preparation. These data will allow a novel understanding of the peripheral regulation of gastric acid secretion as well as the exocytotic process in a non- excitable endocrine cell.

胃肠球菌样细胞（ECL）细胞是负责的细胞 胃基础粘膜内的组胺释放，认为对于 H2受体在顶细胞上的激活。 因此，它发挥了作用 在调节酸分泌方面的核心作用。 它构成1％ 基础上皮的细胞体积。 它已被分离并从大鼠胃中纯化至85％的细胞含量 粘膜，结合洗脱，密度梯度离心 和短期文化。 组胺释放受到胃蛋白/CCK的刺激， 卡尔巴乔和肾上腺素，并被生长抑素H3受体抑制 激动剂和CCK B拮抗剂。 该细胞中刺激分泌耦合的机制尚不清楚。 这些将通过图像分析对细胞内的变化进行研究 钙，氯化物和pH，通过连接的细胞和全细胞测量 并通过筛查定义参与颗粒胞吐作用的蛋白质 源自纯化的ECL细胞制备的cDNA文库。 这些数据将允许对外围调节的新了解 胃酸分泌以及非 - 可激发的内分泌细胞。