Analytical and Biophysical Research Core

分析和生物物理研究核心

• 批准号: 10165746
• 负责人: Joshua S Sharp
• 金额: $ 40.45万
• 依托单位: UNIVERSITY OF MISSISSIPPI
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-15 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10165746
• 关键词: Address; Aerosols; Affinity; Analytical Chemistry; Binding; Bioinformatics; Biological; Biological Assay; Biomedical Research; Biophysics; Carbohydrates; Carbon; Centers of Research Excellence; Characteristics; Charge; Chemistry; Chromatography; Communities; Complex; Complex Mixtures; Computer software; Coupled; Data; Data Analyses; Detection; Development; Diagnostic; Equipment; Experimental Designs; Fluorescence; Glycobiology; Glycoconjugates; Glycosaminoglycans; Glycosides; Growth; High Pressure Liquid Chromatography; Link; Mass Spectrum Analysis; Measures; Methods; Mississippi; Modernization; Modification; Natural Products; Natural graphite; Nature; Neurosciences; Pathway Analysis; Pathway interactions; Phase; Pilot Projects; Polysaccharides; Preparation; Protein-Carbohydrate Interaction; Proteins; Proteome; Proteomics; Research; Research Personnel; Resolution; Resources; Role; Sampling; Science; Services; Spectrophotometry; Statistical Data Interpretation; Statistical Models; Structure; Structure-Activity Relationship; Support System; Surveys; System; Systems Biology; Techniques; Technology; Testing; Training; Universities; X-Ray Crystallography; absorption; base; biophysical analysis; biophysical chemistry; biophysical properties; biophysical techniques; chemical release; chemical resource; computational chemistry; data modeling; depolymerization; design; flexibility; instrumentation; interest; investigator training; metabolome; metabolomics; physical property; programs; tandem mass spectrometry; tool

Project Summary/Abstract The University of Mississippi proposes to establish the Glycoscience Center of Research Excellence (GlyCORE), a Phase I Center of Biomedical Research Excellence. Glycoscience is an essential and rapidly emerging field of biomedical science, and the challenging nature of glycoscience research requires tools and expertise that are not commonly found in the biomedical research community. Moreover, the biomedical community is rapidly growing to appreciate the essential role of carbohydrates, with researchers outside of the self-identified “glycoscience” community finding themselves faced with addressing challenging questions in glycoscience as part of the course of their biomedical researchers. The focus of the Analytical and Biophysical Research Core is three-fold: to train investigators new to glycoscience in proper carbohydrate sample preparation and handling; to support hypothesis-driven research with targeted quantitative analyses from both enriched samples and complex mixtures; and to support discovery-based research to give investigators a system-level view of changes upon perturbation of targeted systems. Training of researchers is a key function of the Core, especially in Phase I when the institutional level of expertise in glycoscience is in its earliest stage of development. Core 1 will train researchers in carbohydrate and carbohydrate conjugate sample preparation techniques. Targeted analyses will be provided as a key technology for hypothesis-driven research, where an investigator wants to test for the presence and quantity of a given glycan or glycoside, structurally characterize an unknown molecule, or validate a key biological or chemical resource. Structural analyses of glycan modification, protein-carbohydrate interactions, binding affinity estimations, and other biophysical analyses will be supported through MS-, LC- and spectrophotometry-based technologies. Access to NMR instrumentation and expertise in spectral acquisition and interpretation will also be provided. Finally, Core 1 will offer “omics”-style discovery-based surveys of glycomes, proteomes, metabolomes, etc. in quantitative, semi-quantitative, and qualitative fashions, supporting systems glycobiology and hypothesis development for targeted assay design. Core 1 will interface closely with the Computational Chemistry and Bioinformatics Core to provide advanced statistical and pathway analyses for “omics” data. The services of Core 1 will use both existing University of Mississippi resources as well as a dedicated high resolution Thermo Q-Exactive Plus LC-MS/MS system and a Dionex Ultimate 3000 analytical UHPLC system. Due to the poor UV-absorption characteristics of most carbohydrates, fluorescence detection for tagged carbohydrates and charged aerosol detection of underivatized carbohydrates will also be available for sensitive detection and quantitation at high dynamic range. By focusing our Phase I Analytical and Biophysical Core on a small number of flexible technologies, we can support the diverse glycoscience interests of UM investigators with cutting-edge technology using a limited array of equipment and have room for growth as GlyCORE matures.

项目概要/摘要 密西西比大学提议建立糖科学卓越研究中心 （GlyCORE）是一期生物医学卓越研究中心，是一个重要且快速发展的中心。 生物医学科学的新兴领域以及糖科学研究的挑战性本质需要工具和 生物医学研究界不常见的专业知识。 人们正在迅速认识到碳水化合物的重要作用，并且外部的研究人员 自我认定的“糖科学”社区发现自己面临着解决具有挑战性的问题 糖科学作为其生物医学研究人员课程的一部分，重点是分析和生物物理学。 研究核心有三部分：在适当的碳水化合物样本中培训糖科学新手 准备和处理；通过有针对性的定量分析来支持假设驱动的研究 丰富的样品和复杂的混合物；并支持基于发现的研究，为研究人员提供帮助 研究人员培训的关键是对目标系统扰动时的变化的系统级看法。 核心的功能，特别是在第一阶段，当糖科学的机构专业水平处于其水平时 核心 1 开发的最早阶段将培训碳水化合物和碳水化合物结合物的研究人员。 样品制备技术将作为假设驱动的关键技术提供。 研究，研究人员想要测试给定聚糖或糖苷的存在和数量， 对未知分子进行结构表征，或验证关键的生物或化学资源。 聚糖修饰分析、蛋白质-碳水化合物相互作用、结合亲和力估计等 基于 MS、LC 和分光光度法的 Access 技术将支持生物物理分析。 最后，还将提供核磁共振仪器以及光谱采集和解释方面的专业知识。 Core 1 将提供“组学”式的基于发现的糖组、蛋白质组、代谢组等调查。 定量、半定量和定性方式，支持系统糖生物学和假设 目标分析设计的开发将与计算化学和 生物信息学核心为“组学”数据提供先进的统计和路径分析服务。 Core 1 将使用密西西比大学现有的资源以及专用的高分辨率 Thermo Q-Exactive Plus LC-MS/MS 系统和 Dionex Ultimate 3000 分析型 UHPLC 系统性能较差。 大多数碳水化合物的紫外吸收特性、标记碳水化合物的荧光检测和 未衍生化碳水化合物的带电气溶胶检测也可用于灵敏检测和 通过将我们的第一阶段分析和生物物理核心集中在一个小型上，在高动态范围内进行定量。 大量灵活的技术，我们可以支持密歇根大学研究人员的不同糖科学兴趣 使用有限设备的尖端技术，随着 GlyCORE 的成熟，还有增长空间。