DIFFERENTIATION IN THE ENDOCRINE AND EXOCRINE PANCREAS

内分泌和外分泌胰腺的分化

• 批准号: 2137551
• 负责人: WILLIAM J RUTTER
• 金额: $ 34.04万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-12-01 至 1996-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2137551
• 关键词: DIFFERENTIATION; ENDOCRINE; EXOCRINE; PANCREAS

DNA probes for individual gene products of both the endocrine pancreas (insulin and potentially glucagon) and the exocrine pancreas (several digestive enzymes including amylase) have been prepared by reverse transcription of mRNA and subsequent cloning in E. coli X1776. We propose to utilize the cloned structural gene sequences to probe the organization and expression of the endocrine and exocrine specific genes. The cloned sequences will be used to isolate (by cloning in E. coli X1776) insulin and amylase gene sequences from rat nuclear DNA. The isolaed genes will permit the study of the sequence organization of the structural genes and their contiguous DNA regions. The cloned structural genes will also be used to study changes in their expression during embryonic development as well as alteration in expression accompanying pathogenic states.

内分泌胰腺的单个基因产物的DNA探针（胰岛素 以及潜在的胰高血糖素）和外分泌胰腺（几种消化酶 包括淀粉酶）是通过mRNA的逆转录和 随后在大肠杆菌X1776中进行克隆。 我们建议利用克隆 结构基因序列探测组织和表达 内分泌和外分泌特异性基因。 克隆序列将用于 分离株（通过在大肠杆菌X1776中克隆）胰岛素和淀粉酶基因序列 大鼠核DNA。 隔离基因将允许研究序列 结构基因的组织及其连续的DNA区域。 这 克隆的结构基因也将用于研究其表达的变化 在胚胎发育期间以及随附表达的变化 致病状态。

项目成果

Expression of rat chymotrypsinogen in yeast: a study on the structural and functional significance of the chymotrypsinogen propeptide.

大鼠胰凝乳蛋白酶原在酵母中的表达：胰凝乳蛋白酶原前肽的结构和功能意义的研究。

• DOI: 10.1016/0014-5793(95)01483-7
• 发表时间: 1996
• 期刊: FEBS letters
• 影响因子: 3.5
• 作者: Venekei,I;Gráf,L;Rutter,WJ
• 通讯作者: Rutter,WJ

Cloning, expression and genomic structure of human LMX1A, and variant screening in Pima Indians.

• DOI: 10.1016/s0378-1119(02)00582-6
• 发表时间: 2002-05
• 期刊: Gene
• 影响因子: 3.5
• 作者: F. Thameem;J. Wolford;Juehu Wang;M. German;C. Bogardus;M. Procházka

A rapid and effective procedure for screening protease mutants.

一种快速有效的筛选蛋白酶突变体的方法。

• DOI: 10.1093/protein/9.1.85
• 发表时间: 1996
• 期刊: Protein engineering
• 作者: Venekei,I;Hedstrom,L;Rutter,WJ
• 通讯作者: Rutter,WJ

Neurogenin3 activates the islet differentiation program while repressing its own expression.

Neurogenin3 激活胰岛分化程序，同时抑制其自身表达。

• DOI: 10.1210/me.2003-0037
• 发表时间: 2004
• 期刊: Molecular endocrinology (Baltimore, Md.)
• 作者: Smith,StuartB;Watada,Hirotaka;German,MichaelS
• 通讯作者: German,MichaelS

Distinct gene expression programs function in progenitor and mature islet cells.

不同的基因表达程序在祖细胞和成熟胰岛细胞中发挥作用。

• DOI: 10.1074/jbc.m213196200
• 发表时间: 2003
• 期刊: The Journal of biological chemistry
• 作者: Watada,Hirotaka;Scheel,DavidW;Leung,Joey;German,MichaelS
• 通讯作者: German,MichaelS