DENTAL PULP RESPONSE TO LOWERED TEMPERATURES

牙髓对低温的反应

• 批准号: 2131122
• 负责人: HAROLD E GOODIS
• 金额: $ 10.45万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-05-01 至 1996-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2131122
• 关键词: Macaca fascicularis; bioengineering /biomedical engineering; biomedical equipment development; cold temperature; cryostat; dental pulp; diagnosis design /evaluation; diagnosis procedure safety; high performance liquid chromatography; histology; human subject; immunocytochemistry; inflammation; microcirculation; monitoring device; neural conduction; neurotransmitters; noninvasive diagnosis; radioimmunoassay; tooth

This project consists of an in vivo, non-invasive investigation of dental pulp cooling, followed by a histological and reversed-phase high performance liquid chromatographic (RP-HPLC) and radioimmunoassay investigation into pulpal neurotransmitters, neuroregulators, and inflammatory regulators as they relate to lowering of pulp temperatures. The specific aims are to initially evaluate the performance of a pulpal cooling device through its application in the M. fascicularis monkey. The device will then be used to determine neural and microcirculatory response non-invasively in humans through concurrent use of a constant current pulp tester and a laser Doppler flow cytometer (LDFC). The response of neurotransmitters, neuroregulators and inflammatory mediators in response to cooling will be examined next. The experimental design involves testing the safety of the device in vivo by applying it to a test animal and examining the tissue histologically for the presence or absence of an inflammatory response. Once it has been established that the device will not harm the pulp, it will be used to alter or abolish interdental nerve activity while evaluating its effect on pulpal microcirculation. The device will be applied to humans to lower pulp temperatures. Concurrent electric pulp testing will be used to assess the levels at which interdental nerve response is altered. Pulpal temperatures will then be lowered to those same levels, while pulpal blood flow is assessed with LDFC. The presence of neurovascular regulators and inflammatory mediators in response to pulp cooling will be identified and quantified by use of RP-HPLC after application of the cooling device in the M. fascicularis monkey. Test results will provide the basis for a cooling device that can be adopted for use clinically to obviate the need of local anesthetics and prevent untoward reactions to dental preparation and restoration procedures.

该项目包括对牙齿的体内无创调查 纸浆冷却，然后是组织学和相反的相位 性能液相色谱（RP-HPLC）和放射免疫测定 研究牙髓神经递质，神经调节剂和 炎症调节剂与降低纸浆温度有关。 具体目的是最初评估牙髓的性能 通过其在M. fascicularis猴子中的应用中进行冷却装置。 然后，该设备将用于确定神经和微循环 通过同时使用常数在人类中无创的反应 电流纸浆测试仪和激光多普勒流式细胞仪（LDFC）。 这 神经递质，神经调节剂和炎症介质的反应 接下来将检查对冷却的响应。 实验设计 涉及通过将其应用于A设备在体内测试设备的安全性 测试动物并在组织学上检查组织是否存在或 没有炎症反应。 一旦确定 该设备不会损害纸浆，它将用于更改或废除 在评估其对牙髓的影响的同时，牙间神经活动 微循环。 该设备将用于人类降低纸浆 温度。 并发的电纸浆测试将用于评估 牙间神经反应的水平发生了变化。 牙髓 然后，温度将降低到相同的水平，而牙髓则 用LDFC评估血流。 神经血管的存在 响应纸浆冷却的调节器和炎症介质将 在应用后，通过使用RP-HPLC来识别和量化 筋膜猴猴子中的冷却装置。 测试结果将提供 可以在临床上使用的冷却设备的基础 消除需要局部麻醉的需求，并防止对 牙科准备和修复程序。