CYTOCHEMISTRY OF CENTRAL AUDITORY PATHWAYS

中枢听觉通路的细胞化学

• 批准号: 2125956
• 负责人: RICHARD L SAINT MARIE
• 金额: $ 19.34万
• 依托单位: HOUSE RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-01 至 1997-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2125956
• 关键词: aspartate; auditory nuclei; auditory pathways; binaural hearing; cats; chinchilla; gamma aminobutyrate; glutamates; glycine; immunochemistry; inferior colliculus; neural information processing; neuroanatomy; neurochemistry; neuronal transport; neuropharmacology; neurotransmitter transport

The long term goal of this project is to understand the structural and neurochemical circuitry of the auditory brainstem in sufficient detail that we can make intelligent structure/function correlations and predict how each part contributes to auditory signal processing. In the present proposal, we will concentrate on the nuclei of the lateral lemniscus (NLL). The NLL provide both monaural and binaural inputs to the auditory midbrain, i.e., the central nucleus of the inferior colliculus (ICc). They are the second largest source of inputs to the ICc (surpassed only by projections from the cochlear nucleus) and are its single largest source of inhibitory inputs. Emerging evidence suggests that inhibition may have many roles in shaping the responses of ICc neurons, including frequency tuning, temporal response patterns, and rate-intensity functions. Inhibition from the dorsal nucleus of the NLL has been implicated in binaural suppression in the ICc. In addition to their main projections to the ICc, the NLL also contribute extralemniscal projections to the auditory thalamus and superior colliculus, and descending projections to the cochlear nucleus and superior olivary complex. Yet, of all regions of the central auditory system, the NLL are the least understood in terms of their cellular composition and cellular connectivity. In the present proposal, we will characterize the cellular architecture of the NLL and correlate these findings with cellular differences in transmitter type and synaptic target. We will also examine the afferent inputs to the crossed and uncrossed projections of the dorsal nucleus of the NLL, so that we can infer something about the types of information being carried to the ICc by each component of its projections. These experiments are designed to bridge the gap between purely morphological and purely neurochemical studies by examining the cytochemistry of morphologically defined neurons in the NLL. In addition, by examining the afferent and efferent connections of these morphologically and cytochemically defined neurons, we hope to learn something about the types of information that each transmits to its synaptic targets. This should better enable us to relate our observations to neurophysiological and neuropharmacological investigations, and to predict how each component of the NLL projections contributes to monaural and binaural signal processing.

该项目的长期目标是了解结构和 听觉脑干的神经化学电路足够详细 我们可以进行智能结构/功能关联并预测 每个部分如何有助于听觉信号处理。在现在 建议，我们将重点关注外侧丘系核 (NLL)。 NLL 为听觉提供单耳和双耳输入 中脑，即下丘中央核（ICc）。他们 是 ICc 的第二大投入来源（仅次于 来自耳蜗核的投影）并且是其最大的单一来源 的抑制性输入。新出现的证据表明，抑制可能会 在塑造 ICc 神经元反应方面发挥着多种作用，包括频率 调谐、时间响应模式和速率-强度函数。 NLL 背核的抑制与 ICc 中的双耳抑制。除了他们的主要预测 ICc、NLL 也为 听觉丘脑和上丘，以及下行投射 耳蜗核和上橄榄复合体。然而，在所有地区中 中枢听觉系统，NLL 在以下方面是最不被理解的 它们的细胞组成和细胞连接性。在现在 提案中，我们将描述 NLL 的蜂窝架构和 将这些发现与发射器类型的细胞差异相关联 突触目标。我们还将检查交叉的传入输入 和 NLL 背核的非交叉投影，这样我们就可以 推断有关传送给 ICc 的信息类型的信息 其预测的每个组成部分。这些实验旨在 弥合纯粹形态学和纯粹神经化学之间的差距 通过检查形态学定义的神经元的细胞化学进行研究 在 NLL 中。此外，通过检查传入和传出 这些形态学和细胞化学定义的神经元的连接， 我们希望了解每个人所获取的信息类型 传输到其突触目标。这应该能让我们更好地联系起来 我们对神经生理学和神经药理学的观察 调查，并预测 NLL 预测的每个组成部分如何 有助于单耳和双耳信号处理。