MOLECULAR REGULATION OF NEUTROPHIL ADHERENCE RECEPTOR

中性粒细胞粘附受体的分子调控

• 批准号: 2143058
• 负责人: DENNIS DURAND HICKSTEIN
• 金额: $ 9.61万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-02-01 至 1996-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2143058
• 关键词: CD antigens; DNA footprinting; RNA biosynthesis; cell differentiation; complementary DNA; deoxyribonuclease I; gene expression; gene induction /repression; genes; genetic mapping; genetic regulation; genetic regulatory element; genetic transcription; leukocyte adhesion molecules; messenger RNA; molecular biology; molecular cloning; myeloid stem cell; neutrophil; nucleic acid methylation; protein sequence; protein structure; receptor binding; receptor expression; retinoate; tissue /cell culture; transfection; CD antigens; DNA footprinting; RNA biosynthesis; cell differentiation; complementary DNA; deoxyribonuclease I; gene expression; gene induction /repression; genes; genetic mapping; genetic regulation; genetic regulatory element; genetic transcription; leukocyte adhesion molecules; messenger RNA; molecular biology; molecular cloning; myeloid stem cell; neutrophil; nucleic acid methylation; protein sequence; protein structure; receptor binding; receptor expression; retinoate; tissue /cell culture; transfection

Candidate: Immediate Career Goal: my immediate career goal is to continue my development as an academic hematologist and independent investigator. My major focus is that of basic research. My principal area of investigation involves the molecular biology of the neutrophil integrin molecules. Candidate: Long-Term Career Goal: My long-term career goal is to devote the majority o my time (80-90%) to basic investigation of myeloid differentiation. Role of FIRST Award in Developuent As An Independent Investigator: The FIRST Award will provide the resources and time for me to pursue my own research ideas in my own laboratory at the Seattle VA Medical Center. Institutions Development Plans and Enviroment: The Seattle VA Medical Center and the Department of medicine at the University of Washington School of Medicine are very supportive of the development of basic research in general, and my research program in particular. The Seattle VA Medical Center is an integral part of the University of Washington Medical Program, and a major aspect of the Seattle VA Medical Center is a Medical Research Service with a number of independent investigators supported by NIH and non-NIH funds. The Seattle VA Medical Center has made a major commitment to the understanding and treatment of hematologic disorders with an active bone marrow transplant facility and five research laboratories studying various aspects of hematopoiesis. In addition, the University of Washington School of Medicine and the Fred Hutchinson Cancer Research Center provide Seattle with a rich diversity for study of hematology and hematologic malignancy. Brief Sununary of Research: The goals of this research are to: 1) identify the regulatory sequences involved in the retinoic acid-induced transcriptional activation of the neutrophil CD18 (beta) subunit gene in differentiating HL-60 promyelocytic cells, and 2) identify the regulatory sequences involved in the retinoic acid-induced stabilization of the CD11b (Mac-lalpha) transcript in differentiating HL-60 cells.

候选人：直接职业目标：我的直接职业目标是 继续我作为学术血液学家和独立的发展 研究者。 我的主要重点是基础研究。 我的校长 研究领域涉及中性粒细胞的分子生物学 整联蛋白分子。 候选人：长期职业目标：我的长期职业目标是投入 我的时间（80-90％）o骨髓的基本研究 分化。 作为独立研究者发展中第一奖的角色： 第一奖将为我提供自己的资源和时间 我自己在西雅图弗吉尼亚州医疗中心的实验室中的研究思想。 机构发展计划和环境：西雅图弗吉尼亚州医疗 华盛顿大学中心和医学系 医学院非常支持基本的发展 总体研究，尤其是我的研究计划。 西雅图 VA医疗中心是华盛顿大学不可或缺的一部分 医疗计划，西雅图弗吉尼亚州医疗中心的主要方面是 与许多独立研究人员的医学研究服务 由NIH和非NIH资金支持。西雅图弗吉尼亚州医疗中心有 对理解和治疗做出了重大承诺 血液学疾病，具有活性的骨髓移植设施和 五个研究造血的研究实验室。 此外，华盛顿大学医学院和 弗雷德·哈钦森癌症研究中心为西雅图提供了丰富的 血液学和血液系统恶性肿瘤研究的多样性。 研究简短的研究：这项研究的目标是：1） 确定视黄酸诱导的调节序列 中性粒细胞CD18（β）亚基基因的转录激活 区分HL-60的临时细胞细胞，2）确定 视黄酸引起的稳定涉及的调节序列 在分化HL-60细胞中的CD11b（MAC-Lalpha）转录本的。