GENETIC RECOMBINATION IN BACILLUS SUBTILIS

枯草芽孢杆菌的基因重组

• 批准号: 2059717
• 负责人: DAVID A DUBNAU
• 金额: $ 29.72万
• 依托单位: PUBLIC HEALTH RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-06-01 至 1997-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2059717
• 关键词: Bacillus subtilis; DNA binding protein; DNA footprinting; DNA replication; autoradiography; bacterial genetics; cell transformation; gel electrophoresis; gene expression; genetic manipulation; genetic mapping; genetic promoter element; genetic recombination; genetic transcription; host organism interaction; laboratory mouse; molecular cloning; mutant; nucleic acid hybridization; nucleic acid sequence; phosphorylation; protein biosynthesis; radiotracer; transcription factor; transposon /insertion element

Genetic competence is defined as the ability of bacterial cells to bind and internalize high molecular weight DNA, which may then be established as an autonomous replicon or recombine with a resident DNA molecule. Competence in Bacillus subtilis is one of several post-exponentially expressed systems, the best characterized of which is sporulation. The present study is directed towards unraveling the complex network of events that determine the onset of competence expression in response to a set of nutritional, growth stage and cell-type specific signals. This network is known to include at least one phosphorylation cascade and possibly several, and included a likely transmembrane signaling event. We will investigate the role of the two-component regulators, ComP and ComQ, in signaling the onset of transcription of srfA, a key intermediate in the regulatory pathway. We will investigate the role of comK in competence regulation and that of MecA, a probable negative regulator of several post-exponential expression systems. We will also attempt to clone and characterize ctf, which appears to encode a transcriptional activator of late competence genes. The interaction of this regulatory pathway with those governing other post-exponential expression systems will also be studied.

遗传能力定义为细菌细胞结合的能力 并内化高分子量DNA，然后可以建立 作为自主复制子或与驻留DNA分子重组。 枯草芽孢杆菌的能力是指数后的几种 表达的系统，其中最佳特征是孢子形成。 这 目前的研究是针对揭示复杂网络的 决定响应能力表达的开始的事件 一组营养，生长阶段和细胞类型的特定信号。 这 已知网络至少包括一个磷酸化级联和 可能有几个，并包括可能的跨膜信号事件。 我们将调查两个组件调节器，Comp和 COMQ，在信号SRFA的转录发作时，关键中间体 在监管途径中。 我们将调查昏迷的作用 能力调节和MECA的调节 几个指数后表达系统。 我们还将尝试 克隆和表征CTF，它似乎编码转录 晚期能力基因的激活因子。 这种调节的相互作用 与其他指数后表达系统的途径 也将研究。