Recovery and recycling of phosphorous from aquatic ecosystems by use of the salt-tolerant green algae Dunaliella and chitosan

利用耐盐绿藻杜氏藻和壳聚糖从水生生态系统中回收和再循环磷

基本信息

批准号：
09650841
负责人：
SUZUKI Takahiro
金额：
$ 1.79万
依托单位：
TOKYO INSTITUTE OF TECHNOLOGY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09650841/
关键词：
micro-algae Dunaliella photosynthesis polyphosphates bio-concentration fixation of P

项目摘要

A basic study on the development of a recovery and recycling process for phosphorous (P) from aquatic ecosystems by use of the salt-tolerant green algae Dunaliella and chitosan was performed. The effects of various combinations of environmental or operational factors on P content in the Dunaliella cells were examined, using laboratory cultures under controlled conditions. The factors examined were the culture temperature, light intensity, CO_2 concentration in flushing gas, P, NaCl, nitrogen and Ca^<2+> concentration in the culture. The experimental system of the factors and the measured responses of algal growth rate and P content was modeled and analyzed based on the response surface method. The model obtained revealed that the intracellular P content in D.parva was highly dependent on the P concentration in the pre-culture medium in the case of the P concentration in the culture was relatively low. It was also found that procaine hydrochloride was useful to increase the growth of the algal cells. In the next step, the P recovery in terms of physico-chemical treatments from the harvested algal cells was studied. The results showed the Ca(0H)_2 or Al_2(SO_4)_3 treatment for the polyphosphates in the algal cells was effective to precipitate phosphate salt. The biopolymers, natural chitin and chitosan, were not so effective at solid removal. Further study on the use of modified chitosan such as iron chitosan will be required.

开展了利用耐盐绿藻杜氏藻和壳聚糖从水生生态系统中回收和再循环磷 (P) 工艺的基础研究。使用受控条件下的实验室培养物检查了环境或操作因素的各种组合对杜氏藻细胞中磷含量的影响。考察的因素是培养温度、光照强度、冲洗气体中的CO_2浓度、培养物中的P、NaCl、氮气和Ca 2+ 浓度。基于响应面法对因素实验体系以及藻类生长速率和磷含量的实测响应进行建模和分析。所得模型表明，在培养物中P浓度较低的情况下，D.parva细胞内P含量高度依赖于预培养基中的P浓度。还发现盐酸普鲁卡因可用于促进藻类细胞的生长。在下一步中，研究了从收获的藻细胞中物理化学处理的磷回收率。结果表明，Ca(0H)_2或Al_2(SO_4)_3处理藻细胞内的聚磷酸盐能有效沉淀磷酸盐。生物聚合物、天然甲壳素和壳聚糖在固体去除方面效果不佳。需要进一步研究改性壳聚糖（例如铁壳聚糖）的使用。