LABORATORY CANCER RESEARCH CENTER

实验室癌症研究中心

• 批准号: 2089154
• 负责人: RAYMOND W RUDDON
• 金额: $ 86.64万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1995-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2089154
• 关键词: biomedical facility; chemical carcinogenesis; biomedical facility; chemical carcinogenesis

The specific aims of the Laboratory Cancer Research Center at the Eppley Institute for Research in Cancer and Allied Diseases are to carry out interdisciplinary research in: 1) cancer cause and prevention aimed at determining the mechanisms of action of chemical carcinogens, including the mechanisms of activation of carcinogens and their interactions with DNA, the role of potential environmental carcinogens in human cancer, the interaction of oncogenes and chemical carcinogens in tumor initiation and promotion, and the role of dietary factors in causing and preventing cancer; 2) molecular and cellular biology , including DNA replication, mechanisms of gene transcription, the mechanisms of action of growth factors and hormones, characterization of growth factor receptors and their signal transduction mechanisms, the role of oncogene expression in cellular differentiation, mechanisms of viral reproduction and oncogene activation, and DNA-protein interactions; and 3) developmental therapeutics, including characterization of tumor markers, isolation and characterization of tumor-associated antigens, development of monoclonal antibodies, development of antisense and triple helical-forming oligonucleotides to inhibit cancer cell growth, synthesis of novel antineoplastic agents, use of peripheral stem cells and cytokines in bone marrow transplantation, and design of protocols for improved drug delivery and pharmacokinetic analysis of anticancer agents. To achieve these specific aims we have assembled a group of 52 faculty with expertise in chemistry, biochemistry, cell biology, molecular biology, immunology, pathology, virology, pharmacology, toxicology, nutrition, and clinical oncology. In support of this research we have established core laboratory facilities in animal care, cell culture, histology, molecular biology including 2-dimensional gel electrophoresis, mass spectrometry, nuclear magnetic resonance spectrometry, computer services including molecular modeling and DNA and protein data base analysis, drug analysis and pharmacokinetics, microbial mutagenesis, glassware washing, and safety. In addition, we have established a Leukemia/Lymphoma Tissue Procurement Service that supports a region-wide program for data collection and protocol standardization. The Core Grant faculty also comprise the nucleus of our Cancer Biology Training Program for the education and research training of predoctoral and postdoctoral students.

Eppley实验室癌症研究中心的具体目的 癌症研究所和盟友疾病研究所应进行 跨学科研究：1）针对的癌症原因和预防 确定化学致癌物的作用机理，包括 致癌物激活的机制及其与 DNA，潜在环境致癌物在人类癌症中的作用， 肿瘤基因和化学致癌物在肿瘤起始和 促进以及饮食因素在引起和预防的作用 癌症; 2）分子和细胞生物学，包括DNA复制， 基因转录机理，生长的作用机理 因素和激素，生长因子受体的表征和 它们的信号转导机制，癌基因表达在 细胞分化，病毒繁殖的机制和癌基因 激活和DNA-蛋白质相互作用； 3）发展 治疗剂，包括肿瘤标记的表征，隔离和 肿瘤相关抗原的表征，单克隆的发展 抗体，反义和三重螺旋形成的发展 寡核苷酸抑制癌细胞生长，合成新型 抗肿瘤剂，骨外周血细胞和细胞因子的使用 骨髓移植和改进药物方案的设计 抗癌剂的递送和药代动力学分析。 为了实现这些特定目标，我们组装了一组52个教职员工 具有化学，生物化学，细胞生物学，分子的专业知识 生物学，免疫学，病理学，病毒学，药理学，毒理学， 营养和临床肿瘤学。 为了支持这项研究 在动物护理，细胞培养中建立的核心实验室设施， 组织学，分子生物学，包括二维凝胶电泳， 质谱法，核磁共振光谱法，计算机 包括分子建模以及DNA和蛋白质数据库的服务 分析，药物分析和药代动力学，微生物诱变， 玻璃器皿清洗和安全。 此外，我们已经建立了 白血病/淋巴瘤组织采购服务支持区域范围 数据收集和协议标准化计划。 核心赠款 教师还包括我们癌症生物学培训计划的核心 进行术前和博士后的教育和研究培训 学生。