Neural mechanism for initiation of labor and maternal behavior

启动分娩和母亲行为的神经机制

基本信息

批准号：
09680795
负责人：
HIGUCHI Takashi
金额：
$ 2.11万
依托单位：
Fukui Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

From our previous results it is known that nitric oxide (NO) given into the cerebroventricle inhibits delivery and maternal behavior in late pregnant rats. This project has started with reexamining NO activity estimated by NADPH diaphorase histochemistry in the brain slices of the previous experiment. We found that contrary to the posterior pituitary gland , NO activity in the anterior pituitary gland is activated during the time of parturition (NeuroReport 8 : 817-821, 1997). Then we examined NO synthase by immunohistochemistiy and NADPH diaphorase histochemistry in the same tissue preparation. We found that some anterior pituitary cells which express NO synthase locate in contact with capillaries. This result indicates that NO in the anterior pituitary gland may be involved in the control of blood flow into the gland (Brain Research 784 : 337-340, 1997). In order to know the role of NO in the central nervous system to control maternal behavior, NO donor was injected into the lateral ventricle of lactating rats. As we had previously reported, milk-ejection reflex was inhibited in these rats and Fos expression in the paraventricular nucleus, supraoptic nucleus and arcuate-median eminence was suppressed. These results suggest that NO regulates activity of neurons in these nuclei to influence anterior pituitary hormones that are involved in controlling these hormonal secretion and initiation of parturition (NeuroReport and Brain Research, in press). We want to pursue the project to develop a new method to evaluate NO expression in each nucleus of the hypothalamus.

从我们之前的结果可知，进入脑室的一氧化氮（NO）会抑制妊娠晚期大鼠的分娩和母性行为。该项目首先重新检查先前实验脑切片中 NADPH 心肌酶组织化学估计的 NO 活性。我们发现，与垂体后叶相反，垂体前叶的 NO 活性在分娩时被激活（NeuroReport 8：817-821，1997）。然后我们在相同的组织制剂中通过免疫组织化学和NADPH心肌酶组织化学检查了NO合酶。我们发现一些表达NO合酶的垂体前叶细胞与毛细血管接触。该结果表明垂体前叶中的NO可能参与控制进入腺体的血流(Brain Research 784：337-340，1997)。为了了解NO在中枢神经系统中控制母体行为的作用，将NO供体注射到哺乳大鼠的侧脑室中。正如我们之前报道的，这些大鼠的排乳反射受到抑制，室旁核、视上核和弓状正中隆起的 Fos 表达也受到抑制。这些结果表明，NO 调节这些细胞核中神经元的活动，从而影响垂体前叶激素，这些激素参与控制这些激素的分泌和分娩的开始（NeuroReport 和 Brain Research，出版中）。我们希望开展该项目，开发一种新方法来评估下丘脑每个核中的 NO 表达。