Comparative systems biology of apicomplexan cell division

顶端复合体细胞分裂的比较系统生物学

• 批准号: 10539938
• 负责人: Manoj T Duraisingh
• 金额: $ 141.38万
• 依托单位: BOSTON COLLEGE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-21 至 2027-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10539938
• 关键词: Apical; Apicomplexa; Atlases; Babesia; Babesiosis; Biological; Biology; Cell Cycle; Cell Cycle Progression; Cell division; Cells; Cellular Assay; Chemicals; Chromatin; Chromosome Segregation; Communities; Computer software; Cyclin-Dependent Kinases; Cyclins; Cytoskeleton; DNA biosynthesis; Data; Data Set; Family; Gene Expression; Gene Expression Profile; Genes; Genetic; Genetic Transcription; Health; Heart; Homologous Gene; Human; Life; Life Style; Malaria; Mammalian Cell; Maps; Mediating; Modeling; Mothers; Online Systems; Opportunistic Infections; Organelles; Parasites; Pathogenesis; Pathway Analysis; Pharmaceutical Preparations; Plasmodium; Plasmodium falciparum; Research; Resolution; Resources; Sarcocystis; Surveys; System; Systems Biology; Time; Toxoplasma gondii; Toxoplasmosis; Transposase; Variant; Visualization; Work; asexual; causal variant; chemical genetics; comparative; epigenomics; experimental study; flexibility; gene network; gene regulatory network; inhibitor; innovation; interest; knock-down; network architecture; novel; nuclear division; programs; query tools; single cell sequencing; single-cell RNA sequencing; software development; tick transmission; transcription factor; transcriptome; transcriptomics; web site

Summary Apicomplexan parasites have major impacts on human health e.g. Plasmodium falciparum causes malaria whereas Toxoplasma gondii and Babesia spp. cause opportunistic infections. Although this close-knit group shares their obligate intracellular life styles, they display a wide variety of asexual cell division modes. These differ between parasites as well as between different life stages within a single parasite species, but the start- and end-point is always a host cell invasion competent ‘zoite’. The number of zoites made per division round varies dramatically (from 2-90,000) and can unfold in several different ways by reshuffling the functional modules of 1) mother cytoskeleton disassembly, 2) DNA synthesis and chromosome segregation (D&S), 3) karyokinesis, and 4) zoite assembly (budding). Distinct cell division modes across Apicomplexa arise from variations in the order and sequence of the modules as well as the number of module repetitions. In the current model, cell division progresses in transcriptional waves mediated transcription factors that act on target genes that in turn bundle into the functional modules. However, little is known of the composition, regulators and wiring of the different modules, and how this leads to the diversity of cell division modes in Apicomplexa. The research team hypothesizes that these questions can be answered by a comparative systems biology approach, starting with parasites representing different diverse and ‘exotic’ division cell division modes wherein particular modules are amplified, or combined differently: Babesia divergens binary fission, P. falciparum schizogony, Sarcocystis neurona endopolygeny without karyokinesis, T. gondii asexual endodyogeny and T. gondii pre-sexual endopolygeny with karyokinesis in the definitive host. This approach takes advantage of the single cell sequencing revolution combined with computational network analysis approaches. Firstly, single cell transcriptomic and epigenomic maps of the five cell division modes will be generated and analyzed to define the effectors contained in each specific module. A subset of uncharacterized effectors in the poorly characterized karyokinesis and cytoskeleton disassembly modules will be experimentally validated by gene knock-downs. Secondly, chemical and genetic perturbations combined with single cell sequencing will enable the assembly of causal gene regulatory networks (GRNs) across all division modes. Candidate module controllers in these GRNs will be validated by reprogramming and/or genetic perturbation experiments: changing (parts of) the division mode in specific parasites. This work will answer elusive questions regarding apicomplexan specific biology within barely studied functional modules, as well as how apicomplexan cell division flexibility is wired. Thirdly, the proposed work will produce extensive community resources comprising single expression and chromatin accessibility atlases across five different cell division modes and parasite species. Moreover, data sets will be searchable across systems in real time for any biological feature of interest by web-based Apps that will be incorporated in VEuPathDB and enable querying the data for biological questions beyond cell division.

概括 顶复门寄生虫对人类健康有重大影响，例如恶性疟原虫引起疟疾 而弓形虫和巴贝虫属会引起机会性感染，尽管这是一个紧密结合的群体。 它们具有专性细胞内生活方式，表现出多种无性细胞分裂模式。 寄生虫之间以及单一寄生虫物种内不同生命阶段之间的差异是不同的，但起始 终点始终是具有宿主细胞侵袭能力的“孢子”，每轮分裂产生的孢子数量。 变化很大（从 2 到 90,000），并且可以通过重新洗牌功能模块以几种不同的方式展开 1) 母细胞骨架拆卸，2) DNA 合成和染色体分离 (D&S)，3) 核分裂， 4) 孢子组装（出芽） 顶复门的不同细胞分裂模式源于不同的细胞分裂模式。 当前模型、单元中模块的顺序和顺序以及模块重复的数量。 分裂在转录波介导的转录因子中进行，这些转录因子作用于靶基因，进而作用于靶基因 然而，人们对它的组成、调节器和接线知之甚少。 不同的模块，以及这如何导致 Apicomplexa 细胞分裂模式的多样性。 发现这些问题可以通过比较系统生物学方法来回答，首先 代表不同的多样化和“异国情调”分裂细胞分裂模式的寄生虫，其中特定模块 放大或以不同方式组合：分歧巴贝斯虫二元裂变、恶性疟原虫分裂生殖、肉孢子虫 无核分裂的神经元内多发生、弓形虫无性内生和弓形虫前性 终宿主中具有核分裂的内多发生这种方法利用了单细胞的优势。 测序革命与计算网络分析方法相结合首先，单细胞。 将生成并分析五种细胞分裂模式的转录组和表观基因组图谱，以定义 每个特定模块中包含的未表征效应器的子集。 核分裂和细胞骨架拆卸模块将通过基因敲除进行实验验证。 其次，将化学和遗传扰动与单细胞测序相结合将能够组装 跨所有分裂模式的因果基因调控网络（GRN）这些GRN中的候选模块控制器。 将通过重新编程和/或遗传扰动实验来验证：改变（部分）分裂 这项工作将回答有关 apicomplexan 特定生物学的难以捉摸的问题。 在几乎没有研究过的功能模块中，以及apicomplexan细胞分裂的灵活性是如何连接的。 拟议的工作将产生广泛的社区资源，包括单一表达和染色质 此外，数据集将是五种不同细胞分裂模式和寄生虫物种的可访问性图集。 可通过基于网络的应用程序跨系统实时搜索任何感兴趣的生物特征 合并到 VEuPathDB 中，并能够查询数据以了解细胞分裂之外的生物学问题。