Yoga Postures and Slow Deep Breathing in Altering Mechanistic Outcomes in Hypertension

瑜伽姿势和缓慢深呼吸改变高血压的机制结果

• 批准号: 10530707
• 负责人: Stacy Denise Hunter
• 金额: $ 18.8万
• 依托单位: TEXAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-12-01 至 2024-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10530707
• 关键词: Accounting; Adult; Aerobic Exercise; Affect; Age; Antioxidants; Biological; Biological Availability; Blood; Blood Pressure; Blood Proteins; Blood Vessels; Blood flow; Breathing; Breathing Exercises; Cardiovascular Diseases; Cause of Death; Cells; Chronic; Clinical; Complex; Coronary Arteriosclerosis; Cutaneous; Data; Disease; Disease Progression; Endothelium; Environment; Enzymes; Free Radicals; Functional disorder; Funding Opportunities; Goals; Health; Homeostasis; Hour; Hypertension; Immune; Immune system; Individual; Interleukin-1 beta; Interleukin-8; Intervention; Locales; Measurement; Measures; Mediating; Molecular; Monocyte Chemoattractant Proteins; NADPH Oxidase; Nitric Oxide; Outcome; Oxidation-Reduction; Oxidative Stress; Pathology; Peripheral Blood Mononuclear Cell; Peripheral Resistance; Physiological; Posture; Proteins; Public Health; Randomized; Randomized, Controlled Trials; Reactive Oxygen Species; Research; Risk; Risk Factors; Role; Seminal; Source; Superoxide Dismutase; Therapeutic Intervention; Thiobarbituric Acid Reactive Substances; Time; Vascular Endothelium; Vasodilation; Visit; Waiting Lists; Yoga; blood pressure elevation; blood pressure reduction; burden of illness; cardiovascular disorder risk; cohort; comparison group; cost; endothelial dysfunction; exercise intervention; follow-up; hemodynamics; hypertensive; immunoregulation; improved; improved outcome; insight; mortality; novel; response; sedentary; shear stress; timeline; trial design

Project Summary Hypertension is a pervasive disease with rising mortality rates in the U.S. Although complex, hypertension pathophysiology entails disruptions in vascular homeostasis driven by increased immune cell reactive oxygen species and subsequent oxidative stress. Yoga's blood pressure-lowering effects have been elucidated; however, the mechanisms are unknown. Our studies have found significant enhancements in flow-mediated dilation, a nitric oxide dependent measure of vascular health, following yoga interventions. As oxidative stress leads to reductions in nitric oxide bioavailability, it is possible that reductions in reactive oxygen species and improvements in redox homeostasis represent a biological mechanism by which yoga improves vascular function. Markers of oxidative stress have improved with yoga; however, immune cells, which are key sources of reactive oxygen species in hypertension, have not been investigated in prior yoga studies. NADPH oxidase expediates disease progression in hypertension via enhanced reactive oxygen species scavenging of nitric oxide, compromised endothelium-dependent vasodilation, and resultant increases in total peripheral resistance. While NADPH oxidase has declined following aerobic exercise interventions, this key enzyme has never been explored in yoga research. The proposed study will investigate immune cell reactive oxygen species and NADPH oxidase as biological mechanisms accounting for yoga's vascular and hemodynamic effects in unmedicated adults with elevated blood pressure or stage I hypertension (Aim 1). In response to 12-week yoga interventions, we will explore reactive oxygen species and NADPH oxidase in peripheral blood mononuclear cells as primary and protein carbonylation, a consequence of oxidative stress, and superoxide dismutase, a marker of antioxidant capacity, as exploratory outcomes. We will also establish a timeline of alterations in molecular, vascular, and hemodynamic outcomes by incorporating measurements at 4-week intervals. We hypothesize that oxidative stress markers will improve in response to the yoga intervention and these changes will be associated with reductions in blood pressure and enhanced endothelial function. As yoga is a composite of postures and slow, deep breathing practices, we will also delineate the contribution of slow deep breathing in modulating these mechanistic outcomes (Sub-Aim 1). Slow, deep breathing alone has resulted in favorable alterations in blood pressure and vascular function. We therefore postulate that slow deep breathing alone will be as effective as yoga in improving BP-related mechanistic outcomes. This study serves as an initial step in the PI's long-term goal of delineating mechanisms by which yoga could lower the risk of or serve as a therapeutic intervention for cardiovascular diseases. This research is significant in that it could establish the role of the immune system in altering clinical outcomes with yoga in hypertensive adults. As oxidative stress also plays a role in other diseases marked by disruptions in vascular homeostasis, results could unveil a mechanism by which yoga could ameliorate other conditions as well.

项目摘要 高血压是一种普遍的疾病，尽管复杂，高血压，但在美国，死亡率上升 病理生理学需要由免疫细胞活性氧驱动的血管稳态中断 物种和随后的氧化应激。已经阐明了瑜伽的降压作用； 但是，机制是未知的。我们的研究发现流介导的显着增强 瑜伽干预后的扩张，一种一氧化氮依赖性血管健康的度量。作为氧化应激 导致一氧化氮生物利用度的降低，活性氧和 氧化还原稳态的改善代表了一种生物学机制，瑜伽可以改善血管 功能。瑜伽的氧化应激标记得到了改善。但是，免疫细胞是关键来源 在先前的瑜伽研究中，尚未对高血压中的活性氧。 NADPH氧化酶 通过增强的活性氧对一氮进行增强的疾病进展 氧化物，依赖内皮依赖性的血管舒张，导致的总周围电阻增加。 尽管有氧运动干预措施后，NADPH氧化酶已经下降了，但该关键酶从未有过 在瑜伽研究中探索。拟议的研究将研究免疫细胞活性氧和NADPH 氧化酶作为生物学机制，这些机制考虑了瑜伽的血管和血流动力学作用 血压或I期高血压升高的成年人（AIM 1）。为了应对12周的瑜伽干预措施， 我们将探索外周血单核细胞中的活性氧和NADPH氧化酶作为主要 和蛋白质羰基化，氧化应激的结果和超氧化物歧化酶，抗氧化剂的标志物 能力，作为探索结果。我们还将建立一个分子，血管和血管改变的时间表 通过以4周的间隔合并测量来结合血液动力学结局。我们假设该氧化 应力标记将根据瑜伽干预的响应而改善，这些变化将与 血压降低和内皮功能增强。由于瑜伽是姿势和缓慢的组合，所以 深呼吸的做法，我们还将描绘出缓慢深呼吸的贡献 机械结果（Sub-aim 1）。慢速，深呼吸就导致了血液的有利改变 压力和血管功能。因此，我们假设单独的缓慢呼吸将像 瑜伽改善与BP相关的机理结果。这项研究是PI长期的第一步 划定瑜伽可以降低或用作治疗干预措施的瑜伽的目标的目标 心血管疾病。这项研究很重要，因为它可以确定免疫系统在 在高血压成年人中改变瑜伽的临床结果。由于氧化应激也在其他疾病中起作用 以血管稳态的干扰为标志，结果可以揭示出瑜伽可以通过的机制 还可以改善其他条件。