Biological Soft X-ray Tomography at the Advanced Light Source

先进光源下的生物软 X 射线断层扫描

• 批准号: 10491185
• 负责人: Carolyn A Larabell
• 金额: $ 88.57万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10491185
• 关键词: 3-Dimensional; Adherence; Advisory Committees; Algorithmic Software; Area; Beer; Biochemical; Biological; Biomedical Research; Biophysics; Calibration; Cell Size; Cells; Cellular biology; Chemicals; Communication; Communities; Computer software; Contrast Media; Cryopreservation; Data; Data Analyses; Data Collection; Development; Diabetes Mellitus; Disease; Disseminated Malignant Neoplasm; Education and Outreach; Electron Microscopy; Ensure; Eukaryotic Cell; Feedback; Fluorescence; Fluorescence Microscopy; Funding; Future; Home; Hour; Human; Image; Imaging Techniques; Imaging technology; Individual; Infrastructure; Institution; Laboratories; Laws; Length; Light; Maintenance; Microscope; Modeling; Molecular Machines; Monitor; Neurodegenerative Disorders; Online Systems; Organism; Parasites; Peer Review; Persons; Preparation; Process; Questionnaires; Reporting; Research; Research Personnel; Resolution; Resources; Roentgen Rays; Running; Safety; Schedule; Services; Shapes; Site; Source; Specimen; Stains; Synchrotrons; System; Technology; Thick; Time; Training; Training Programs; Visit; Work; X-Ray Tomography; absorption; aged; attenuation; base; beamline; biomedical facility; biomedical imaging; cell type; cellular imaging; computerized data processing; computing resources; contrast imaging; cryogenics; end of life; experience; human disease; human imaging; imaging modality; improved; insight; instrument; instrumentation; microscopic imaging; novel therapeutics; operation; outreach; programs; ranpirnase; reconstruction; repaired; tomography; transmission process; user-friendly; 3-Dimensional; Adherence; Advisory Committees; Algorithmic Software; Area; Beer; Biochemical; Biological; Biomedical Research; Biophysics; Calibration; Cell Size; Cells; Cellular biology; Chemicals; Communication; Communities; Computer software; Contrast Media; Cryopreservation; Data; Data Analyses; Data Collection; Development; Diabetes Mellitus; Disease; Disseminated Malignant Neoplasm; Education and Outreach; Electron Microscopy; Ensure; Eukaryotic Cell; Feedback; Fluorescence; Fluorescence Microscopy; Funding; Future; Home; Hour; Human; Image; Imaging Techniques; Imaging technology; Individual; Infrastructure; Institution; Laboratories; Laws; Length; Light; Maintenance; Microscope; Modeling; Molecular Machines; Monitor; Neurodegenerative Disorders; Online Systems; Organism; Parasites; Peer Review; Persons; Preparation; Process; Questionnaires; Reporting; Research; Research Personnel; Resolution; Resources; Roentgen Rays; Running; Safety; Schedule; Services; Shapes; Site; Source; Specimen; Stains; Synchrotrons; System; Technology; Thick; Time; Training; Training Programs; Visit; Work; X-Ray Tomography; absorption; aged; attenuation; base; beamline; biomedical facility; biomedical imaging; cell type; cellular imaging; computerized data processing; computing resources; contrast imaging; cryogenics; end of life; experience; human disease; human imaging; imaging modality; improved; insight; instrument; instrumentation; microscopic imaging; novel therapeutics; operation; outreach; programs; ranpirnase; reconstruction; repaired; tomography; transmission process; user-friendly

Project Summary/Abstract The National Center for X-ray Tomography (NCXT) is a full-service, user facility for biomedical imaging and research. NCXT Users, whether working on site or remotely, have access to specialized microscopes and imaging techniques, in particular soft x-ray tomography (SXT) and super-resolution cryogenic fluorescence tomography (SR-CFT). In the US, these imaging modalities are only available at the NCXT. SXT microscopes visualize and quantify the mesoscale organization of biological cells, including large eukaryotic cells. No stains or contrast agents are required for SXT imaging. Before being imaged by the NCXT's transmission soft x-ray microscope at the Advanced Light Source, cells are rapidly cryo-preserved; the only pre-imaging step required. SXT is highly complementary to other imaging modalities, particularly fluorescence and electron microscopies. Mesoscale, 3D SXT reconstructions of an entire cell act as a framework for the interpretation of other types of imaging and biophysical data, allowing them to be viewed in the context of the whole cell. XM2 is currently the only microscope of its kind in the Nation. The requested P30 funding will support the day-to-day operation of XM-2, together with the associated user program and specimen preparation laboratories, and a program of upgrades to our software/computing resources. During the funding cycle, we will achieve the following specific aims: Aim 1: Give the biomedical research community access to soft x-ray tomography (SXT). The work of this aim ensures imaging cells by SXT remains an option open to US researchers. Significant consideration will be given to making the Resource as user-friendly as possible, particular towards the community of researchers that work remotely. Aim 2: Maintain and calibrate XM-2, the NCXT operated soft x-ray microscope at the ALS. As with all synchrotron beamlines, XM-2 is a sophisticated instrument that requires regular maintenance and calibration. We will take a programmatic approach to ensure XM-2 is robust, reliable, and operates with minimum unscheduled downtime. Aim 3: Implement upgrades and enhancements to ensure the NCXT remains `state-of-the-art.' Over the timescale of the funding cycle, new hardware, software, and algorithms will become available, and some existing components will reach their end-of-life. We will, therefore, carry out upgrades and enhancements to maintain the NCXT at the `state-of-the-art.'

项目摘要/摘要 国家X射线断层扫描中心（NCXT）是一个全方位服务的用户设施 生物医学成像和研究。 NCXT用户，无论是在网站上还是远程工作， 有权访问专用显微镜和成像技术，特别是软X射线 断层扫描（SXT）和超分辨率低温荧光 断层扫描（SR-CFT）。在美国，这些成像方式仅在 NCXT。 SXT显微镜可视化和量化生物细胞的中尺度组织， 包括大型真核细胞。 SXT不需要污渍或对比剂 成像。在由NCXT的传输软X射线显微镜成像之前 晚期光源，细胞得到迅速保存；唯一的预示步骤 必需的。 SXT高度互补与其他成像方式，尤其是荧光和 电子显微镜。中尺度，整个细胞的3D SXT重建作为一个 解释其他类型的成像和生物物理数据的框架， 允许它们在整个单元格的上下文中查看。 XM2目前是全国唯一的同类显微镜。请求的P30 资金将支持XM-2的日常操作，以及相关的 用户程序和标本准备实验室，以及升级程序 软件/计算资源。在资金周期中，我们将实现以下内容 具体目的： 目标1：让生物医学研究社区获得软X射线 断层扫描（SXT）。该目标的工作确保SXT成像单元格仍然是 对美国研究人员开放的选项。将有重大考虑 尽可能用户友好的资源，尤其是针对研究人员社区 这项工作遥不可及。 AIM 2：维护和校准XM-2，NCXT运行的软X射线 显微镜在ALS。与所有同步束线一样，XM-2是一个复杂的 需要定期维护和校准的仪器。我们将接受 确保XM-2的程序化方法可靠，可靠，并且以最少的速度运行 未定的停机时间。 目标3：实施升级和增强功能以​​确保NCXT 仍然是“最先进的”。在资金周期的时间尺度上，新硬件， 软件和算法将可用，一些现有组件将 到达他们的终止。因此，我们将进行升级和增强 将NCXT保持在“最先进的”处。