High Spatiotemporal Resolution Neural Recording System Using Active Sensing

使用主动传感的高时空分辨率神经记录系统

基本信息

批准号：
10481444
负责人：
Youbo Zhao
金额：
$ 89.03万
依托单位：
PHYSICAL SCIENCES, INC
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-05-01 至 2024-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10481444
关键词：
Action Potentials Animals Architecture Base of the Brain Brain Calcium Cellular Structures Collaborations Complex Computer software Data Data Collection Dendrites Detection Devices Electronics Electrophysiology (science)Engineering Feedback Fiber Fluorescence Future Generations Head Human Image Imaging Device Imaging technology In Vitro Institutes Investigation Kinetics Label Laboratory Research Light Marketing Measures Membrane Potentials Mental disorders Microscope Neurons Neurosciences Neurosciences Research Noise Optics Performance Phase Population Preparation Publishing Research Resolution Scientist Signal Transduction Slice Speed Structure Synapses System Technology Test Result Testing Universities Update Wisconsin base brain research cognitive function college commercialization cost design detector experimental study fluorescence imaging imager imaging approach improved in vivo innovation instrument interest microscopic imaging millisecond multidisciplinary nervous system disorder neural network neuronal cell body neurotransmission novel novel imaging technique operation optical imaging performance tests physical science programs prototype quasar relating to nervous system research and development sensor spatiotemporal submicron success temporal measurement tool voltage voltage sensitive dye

Action Potentials Animals Architecture Base of the Brain Brain Calcium Cellular Structures Collaborations Complex Computer software Data Data Collection Dendrites Detection Devices Electronics Electrophysiology (science)Engineering Feedback Fiber Fluorescence Future Generations Head Human Image Imaging Device Imaging technology In Vitro Institutes Investigation Kinetics Label Laboratory Research Light Marketing Measures Membrane Potentials Mental disorders Microscope Neurons Neurosciences Neurosciences Research Noise Optics Performance Phase Population Preparation Publishing Research Resolution Scientist Signal Transduction Slice Speed Structure Synapses System Technology Test Result Testing Universities Update Wisconsin base brain research cognitive function college commercialization cost design detector experimental study fluorescence imaging imager imaging approach improved in vivo innovation instrument interest microscopic imaging millisecond multidisciplinary nervous system disorder neural network neuronal cell body neurotransmission novel novel imaging technique operation optical imaging performance tests physical science programs prototype quasar relating to nervous system research and development sensor spatiotemporal submicron success temporal measurement tool voltage voltage sensitive dye

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项目摘要

Project Summary/Abstract The investigation of the complex neural dynamics and the cognitive functions of the brain requires non- invasive recording tools with high spatial and temporal resolution. Fluorescence imaging/microscopy is one of the state-of-the-art technologies for high spatial resolution recording of the activity of neuron populations. However, existing fluorescence neural imaging technologies generally have limited speed, providing less than a few hundred frames per second (or several milliseconds temporal resolution). This is not only limited by the technology barriers (e.g. the low speed of cameras and/or beam scanners), but also constrained by the low signal level emitted by the delicate micro-scale neuronal structures. The milliseconds or slower temporal resolution substantially precludes measuring the precise timing of the generation and propagation of neuron spikes, which is the key component of neural signaling. During this R&D program, Physical Sciences Inc. (PSI), Dartmouth College, and the Broad Institute of MIT and Harvard propose to develop and demonstrate a novel fluorescence neural imaging technology that enables high-speed recording of membrane potentials from multiple neurons. This technology combines two complementary imaging channels to achieve parallel neuronal recording with both sub-micron spatial and sub-millisecond temporal resolution. The high-speed recording function is achieved using a novel imaging technique based on a high-sensitivity single-point detector and a high-speed spatial light modulator (SLM). During the Phase I, we demonstrated the feasibility of the technology by imaging cultured neurons labeled with calcium and voltage indicating fluorescent sensors. During the proposed Phase II, we will upgrade the technology and further demonstrate its value in neuroscience investigations. The Phase II prototypes will include a universal high spatiotemporal resolution sensor that is compatible with various imaging setups including head-mounted fluorescence mini-microscopes. Two Phase II prototypes will be delivered to collaborating institutes for performance testing. The testing experiments will focus on demonstrating high spatiotemporal resolution recording of fast action potentials from both neuron somas in the brain in vivo and sub-cellular structures (e.g., dendrites and synapses) of neuron cultures or brain slices using genetically encoded voltage sensors. This R&D project will result in a robust technology for non-invasive recording of neuronal kinetics with high spatiotemporal resolution, offering a greatly needed tool in the neuroscience field.

项目摘要/摘要对复杂的神经动力学和大脑的认知功能的研究需要非具有高空间和时间分辨率的侵入性记录工具。荧光成像/显微镜是一个神经元种群活动的高空间分辨率记录的最新技术。但是，现有的荧光神经成像技术通常的速度有限，提供的速度较少每秒几百帧（或几毫秒的时间分辨率）。这不仅有限通过技术障碍（例如，摄像机和/或光束扫描仪的低速），但也受到约束微妙的微尺度神经元结构发出的低信号水平。毫秒或较慢时间分辨率基本上无法测量生成和传播的精确时机神经元尖峰，这是神经信号传导的关键组成部分。在此研发计划中，物理科学公司（PSI），达特茅斯学院和麻省理工学院和哈佛大学的广泛研究所提议开发和演示一种新型的荧光神经成像技术，可实现高速记录来自多个神经元的膜电位。该技术结合了两个补充成像通道以获得亚微米空间和亚毫米的平行神经元记录时间分辨率。高速记录函数是使用基于新型成像技术实现的在高敏性单点检测器和高速空间光调节器（SLM）上。在第一阶段，我们通过成像带有钙和标记的培养神经元和电压表示荧光传感器。在拟议的第二阶段中，我们将升级技术和进一步证明了其在神经科学研究中的价值。 II期原型将包括通用高时空分辨率传感器，与各种成像设置兼容，包括头部安装荧光迷你微观。两期II期原型将交付给合作机构性能测试。测试实验将集中于证明高时空分辨率记录体内神经元的快速动作电位，体内和细胞下结构（例如，使用遗传编码的电压传感器的神经元培养物或脑切片的树突和突触）。该研发项目将为非侵入性记录神经元动力学带来强大的技术时空分辨率，在神经科学领域提供了非常需要的工具。