Core B: Mouse Metabolism and Imaging

核心 B：小鼠代谢和成像

• 批准号: 10457901
• 负责人: Allison W Xu
• 金额: $ 18.68万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-01 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10457901
• 关键词: Address; Animal Model; Animals; Archaea; Area; Bacteria; Blood Pressure; Body Composition; Body Temperature; Body Water; Body measure procedure; Bone Density; Bone Mineral Contents; Breeding; Cells; Charge; Collection; Communities; Complement; Complex; Computer software; Conscious; Cultured Cells; Disease; Eating; Effectiveness; Energy Intake; Energy Metabolism; Ensure; Equipment; Extramural Activities; Fatty acid glycerol esters; Feces; Feeding Patterns; Feeding behaviors; Functional disorder; Genetic; Genomics; Genus Hippocampus; Germ-Free; Glycolysis; Gnotobiotic; Goals; Grant; Homeostasis; Human; Image; Incubators; Influentials; Intervention; Islets of Langerhans; Laboratory mice; Lipids; Longitudinal Studies; Magnetic Resonance Imaging; Maintenance; Measurement; Measures; Metabolic; Metabolic Diseases; Metabolism; Methodology; Methods; Microscopic; Mitochondria; Modeling; Monitor; Motor Activity; Mus; Nutritional; Obesity; Organelles; Output; Oxygen Consumption; Paper; Parasites; Pharmacology; Physiologic Thermoregulation; Physiological; Physiology; Rattus; Reagent; Reproducibility; Research; Research Methodology; Research Personnel; Resources; Respiration; Rodent; Rodent Model; Roentgen Rays; Running; San Francisco; Scientist; Series; Services; Study models; System; Technology; Temperature; Therapeutic; Thinness; Time; Tissue Sample; Tissues; Training; Urine; Virus; Work; base; behavioral study; blood glucose regulation; density; design; disorder risk; epidemiology study; extracellular; feeding; food consumption; fungus; human tissue; in vivo; innovation; instrument; instrumentation; investigator training; member; metabolic abnormality assessment; metabolic phenotype; metabolic rate; microbiome; microorganism; nutrition; obesity development; operation; research study; respiratory; success; temporal measurement; time use; tool

Biomedical Core B (Mouse Metabolism): Summary The study of obesity, nutrition, and metabolism relies on methods to measure feeding patterns, metabolic rate, activity, body composition, and fat distribution. Model organisms are a mainstay in the study of metabolism, obesity, and its consequences. Laboratory mice are the most fundamental and common models for studying in vivo physiology and disease pathophysiology, and the UCSF NORC Biomedical Mouse Metabolism Core (Core B) is specifically geared to measure a wide variety of metabolic parameters in mice, as well as in mouse and human tissues, and in cultured cells. The information gained by studying mice, which are often genetically modified through use of Core C (Genetics and Genomics) and examined in conjunction with epidemiological and behavioral studies done in humans (Core A), is essential to understand mechanisms underlying metabolic disease risk, and the potential effectiveness of nutritional and pharmacologic approaches to ameliorate obesity and its consequences. 21 of the 58 investigators in the proposed UCSF-NORC currently conduct research studies in which rodent models are monitored using the types of equipment and analytical approaches housed in Core B. Another 4 NORC investigators indicate that their research has developed to a point where their use of facilities in Core B is imminent. Experts in charge of running Core B keep abreast of the rapidly evolving application of these sophisticated methods, and ensure that NORC researchers are trained in their proper implementation. The presence of the facilities, and the availability of NORC support that is designed to assist the entry of NORC researchers into technologically unfamiliar areas, ensure the success of UCSF-NORC research. Core B provides access to, assistance with, and training in the use of sophisticated methods and instruments for those studies. Specifically, Core B provides tools and facilities for: 1. Precise and real-time measurement of feeding, energy expenditure, locomotor activities, respiratory exchange ratio, and thermoregulation in conscious mice. 2. Analyzing lean mass, fat mass, free and total body water in conscious mice, as well as bone mineral density and other aspects of body composition in anesthetized mice. 3. Cellular energetic measurements using the Seahorse FX24 Bioanalyzer, which measures the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells and tissues in a 24-well plate format. OCR and ECAR are key indicators of mitochondrial respiration and glycolysis, and provide a systems- level view of cellular metabolic function in cells and ex-vivo tissue samples from model organisms and humans. 4. Equipment for urine and fecal collection and blood pressure measurement. 5. Rodent incubators that allow for the study of mice under cold ambient or warm thermoneutral conditions. 6. Gnotobiotics service. Gnotobiotic technology enables breeding and maintenance of germ-free animals devoid of any associated microorganisms, including bacteria, archaea, microscopic fungi, parasites, and viruses. Most importantly, Core B provides non-invasive methods that permit mice to be followed over time. The ability to conduct longitudinal studies in mice is particularly significant for tracking the changes in body composition, feeding behavior, and metabolic activity that both trigger and respond to the development of obesity and its associated complications. Overall, this Core significantly lowers the methodologic barriers to help NORC researchers achieve the efficient and proper application of a series of highly sophisticated tools. These tools accelerate a variety of diverse and interrelated studies in obesity, nutrition, ingestive behavior, and metabolism.

生物医学核心 B（小鼠代谢）：总结 对肥胖、营养和新陈代谢的研究依赖于测量喂养模式、代谢率、 活动、身体成分和脂肪分布。模式生物是新陈代谢研究的支柱， 肥胖及其后果。实验小鼠是最基本、最常见的研究模型 体内生理学和疾病病理生理学，以及 UCSF NORC 生物医学小鼠代谢核心 （核心 B）专门用于测量小鼠以及小鼠体内的各​​种代谢参数 和人体组织以及培养细胞中。通过研究小鼠获得的信息，这些小鼠通常具有遗传性 通过使用核心 C（遗传学和基因组学）进行修改并结合流行病学进行检查 和在人类中进行的行为研究（核心 A）对于理解代谢背后的机制至关重要 疾病风险以及营养和药物方法改善肥胖的潜在有效性 及其后果。 拟议的 UCSF-NORC 的 58 名研究人员中，有 21 名目前正在进行啮齿动物研究 使用核心 B 中的设备类型和分析方法来监控模型。另外 4 NORC 调查人员表示，他们的研究已经发展到可以使用 Core B 中的设施的程度 迫在眉睫。负责运行 Core B 的专家随时了解这些快速发展的应用程序 复杂的方法，并确保 NORC 研究人员接受正确实施的培训。这 设施的存在，以及旨在协助 NORC 进入的 NORC 支持的可用性 研究人员进入技术不熟悉的领域，确保 UCSF-NORC 研究的成功。 核心 B 提供使用复杂方法和仪器的机会、帮助和培训 对于那些研究。具体来说，Core B 提供以下工具和设施： 1. 精确实时测量进食、能量消耗、运动活动、呼吸 交换率和清醒小鼠的体温调节。 2. 分析清醒小鼠的瘦肉质量、脂肪质量、游离水和全身水以及骨矿物质 麻醉小鼠的密度和身体成分的其他方面。 3. 使用 Seahorse FX24 生物分析仪进行细胞能量测量，该分析仪可测量氧气 24孔板中活细胞和组织的消耗率（OCR）和细胞外酸化率（ECAR） 格式。 OCR 和 ECAR 是线粒体呼吸和糖酵解的关键指标，并提供了一个系统- 来自模型生物体和人类的细胞和离体组织样本中细胞代谢功能的水平视图。 4、尿液、粪便收集和血压测量设备。 5. 啮齿动物培养箱，允许在寒冷环境或温暖中性条件下研究小鼠。 6. 知生服务。知生技术使无菌动物的繁殖和维护成为可能 不含任何相关微生物，包括细菌、古细菌、微小真菌、寄生虫和 病毒。 最重要的是，Core B 提供了非侵入性方法，允许长期跟踪小鼠。能力 在小鼠身上进行纵向研究对于追踪身体成分的变化尤为重要， 喂养行为和代谢活动都会引发和响应肥胖及其相关疾病的发展 相关并发症。总体而言，该核心显着降低了帮助 NORC 的方法障碍 研究人员实现了一系列高度复杂的工具的高效和正确应用。这些工具 加速肥胖、营养、摄入行为和新陈代谢方面的各种多样化且相互关联的研究。