Core B: Mouse Metabolism and Imaging

核心 B：小鼠代谢和成像

• 批准号: 10457901
• 负责人: Allison W Xu
• 金额: $ 18.68万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-01 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10457901
• 关键词: Address; Animal Model; Animals; Archaea; Area; Bacteria; Blood Pressure; Body Composition; Body Temperature; Body Water; Body measure procedure; Bone Density; Bone Mineral Contents; Breeding; Cells; Charge; Collection; Communities; Complement; Complex; Computer software; Conscious; Cultured Cells; Disease; Eating; Effectiveness; Energy Intake; Energy Metabolism; Ensure; Equipment; Extramural Activities; Fatty acid glycerol esters; Feces; Feeding Patterns; Feeding behaviors; Functional disorder; Genetic; Genomics; Genus Hippocampus; Germ-Free; Glycolysis; Gnotobiotic; Goals; Grant; Homeostasis; Human; Image; Incubators; Influentials; Intervention; Islets of Langerhans; Laboratory mice; Lipids; Longitudinal Studies; Magnetic Resonance Imaging; Maintenance; Measurement; Measures; Metabolic; Metabolic Diseases; Metabolism; Methodology; Methods; Microscopic; Mitochondria; Modeling; Monitor; Motor Activity; Mus; Nutritional; Obesity; Organelles; Output; Oxygen Consumption; Paper; Parasites; Pharmacology; Physiologic Thermoregulation; Physiological; Physiology; Rattus; Reagent; Reproducibility; Research; Research Methodology; Research Personnel; Resources; Respiration; Rodent; Rodent Model; Roentgen Rays; Running; San Francisco; Scientist; Series; Services; Study models; System; Technology; Temperature; Therapeutic; Thinness; Time; Tissue Sample; Tissues; Training; Urine; Virus; Work; base; behavioral study; blood glucose regulation; density; design; disorder risk; epidemiology study; extracellular; feeding; food consumption; fungus; human tissue; in vivo; innovation; instrument; instrumentation; investigator training; member; metabolic abnormality assessment; metabolic phenotype; metabolic rate; microbiome; microorganism; nutrition; obesity development; operation; research study; respiratory; success; temporal measurement; time use; tool

Biomedical Core B (Mouse Metabolism): Summary The study of obesity, nutrition, and metabolism relies on methods to measure feeding patterns, metabolic rate, activity, body composition, and fat distribution. Model organisms are a mainstay in the study of metabolism, obesity, and its consequences. Laboratory mice are the most fundamental and common models for studying in vivo physiology and disease pathophysiology, and the UCSF NORC Biomedical Mouse Metabolism Core (Core B) is specifically geared to measure a wide variety of metabolic parameters in mice, as well as in mouse and human tissues, and in cultured cells. The information gained by studying mice, which are often genetically modified through use of Core C (Genetics and Genomics) and examined in conjunction with epidemiological and behavioral studies done in humans (Core A), is essential to understand mechanisms underlying metabolic disease risk, and the potential effectiveness of nutritional and pharmacologic approaches to ameliorate obesity and its consequences. 21 of the 58 investigators in the proposed UCSF-NORC currently conduct research studies in which rodent models are monitored using the types of equipment and analytical approaches housed in Core B. Another 4 NORC investigators indicate that their research has developed to a point where their use of facilities in Core B is imminent. Experts in charge of running Core B keep abreast of the rapidly evolving application of these sophisticated methods, and ensure that NORC researchers are trained in their proper implementation. The presence of the facilities, and the availability of NORC support that is designed to assist the entry of NORC researchers into technologically unfamiliar areas, ensure the success of UCSF-NORC research. Core B provides access to, assistance with, and training in the use of sophisticated methods and instruments for those studies. Specifically, Core B provides tools and facilities for: 1. Precise and real-time measurement of feeding, energy expenditure, locomotor activities, respiratory exchange ratio, and thermoregulation in conscious mice. 2. Analyzing lean mass, fat mass, free and total body water in conscious mice, as well as bone mineral density and other aspects of body composition in anesthetized mice. 3. Cellular energetic measurements using the Seahorse FX24 Bioanalyzer, which measures the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells and tissues in a 24-well plate format. OCR and ECAR are key indicators of mitochondrial respiration and glycolysis, and provide a systems- level view of cellular metabolic function in cells and ex-vivo tissue samples from model organisms and humans. 4. Equipment for urine and fecal collection and blood pressure measurement. 5. Rodent incubators that allow for the study of mice under cold ambient or warm thermoneutral conditions. 6. Gnotobiotics service. Gnotobiotic technology enables breeding and maintenance of germ-free animals devoid of any associated microorganisms, including bacteria, archaea, microscopic fungi, parasites, and viruses. Most importantly, Core B provides non-invasive methods that permit mice to be followed over time. The ability to conduct longitudinal studies in mice is particularly significant for tracking the changes in body composition, feeding behavior, and metabolic activity that both trigger and respond to the development of obesity and its associated complications. Overall, this Core significantly lowers the methodologic barriers to help NORC researchers achieve the efficient and proper application of a series of highly sophisticated tools. These tools accelerate a variety of diverse and interrelated studies in obesity, nutrition, ingestive behavior, and metabolism.

生物医学核心B（老鼠代谢）：摘要 肥胖，营养和代谢的研究依赖于测量进食模式，代谢率， 活动，身体成分和脂肪分布。模型生物是新陈代谢研究的主要主体 肥胖及其后果。实验室小鼠是研究中最基本和最常见的模型 体内生理学和疾病病理生理学以及UCSF NORC生物医学小鼠代谢核心 （核B）专门用于测量小鼠的各种代谢参数，以及小鼠 和人体组织，以及培养的细胞。通过研究小鼠获得的信息，这些信息通常是遗传学上的 通过使用核心C（遗传学和基因组学）修改并与流行病学一起检查 在人类中进行的行为研究（核心A）对于了解代谢的机制至关重要 疾病的风险以及营养和药理学方法的潜在有效性来改善肥胖症 及其后果。 在拟议的UCSF-NORC中的58位研究人员中，有21个目前正在进行研究研究 使用核心B中的设备类型和分析方法对模型进行监测。另外4个 NORC调查人员指出，他们的研究已经发展到他们在核心B中使用设施的地步 迫在眉睫。负责运行核心B的专家保持与这些的快速发展的同步 精致的方法，并确保对NORC的研究人员进行适当的实施培训。这 设施的存在以及旨在协助NORC进入的NORC支持的可用性 研究人员在技术上不熟悉的领域，确保UCSF-NORC研究的成功。 核心B提供了使用复杂方法和工具的使用，协助和培训 对于这些研究。具体而言，Core B提供了以下工具和设施： 1。喂养，能量消耗，运动活动，呼吸系统的精确和实时测量 交换比率和有意识小鼠的温度调节。 2。分析有意识的小鼠中的瘦质量，脂肪质量，自由和全身水以及骨矿物质 麻醉小鼠的密度和身体成分的其他方面。 3。使用Seahorse FX24生物分析仪进行细胞能量测量，该测量仪测量氧气 24孔板中活细胞和组织的消耗率（OCR）和细胞外酸化速率（ECAR） 格式。 OCR和ECAR是线粒体呼吸和糖酵解的关键指标，并提供系统 - 细胞代谢功能的水平视图在模型生物和人类中的细胞和前体组织样品中的视图。 4。尿液和粪便收集和血压测量的设备。 5。啮齿动物孵化器，可以在冷环境或温暖的热中性条件下研究小鼠。 6。gnotobiotics服务。 gnotobiotic技术可实现无菌动物的繁殖和维护 没有任何相关的微生物，包括细菌，古细菌，微观真菌，寄生虫和 病毒。 最重要的是，核心B提供了无创的方法，可以随着时间的推移遵循小鼠。能力 在小鼠中进行纵向研究对于跟踪人体组成的变化特别重要， 喂养行为和代谢活动既触发又对肥胖的发展做出反应 相关并发症。总体而言，该核心大大降低了帮助NORC的方法论障碍 研究人员实现了一系列高度复杂的工具的有效和正确应用。这些工具 加快肥胖，营养，摄取行为和代谢的各种不同和相互关联的研究。