Zeiss 980 confocal microscope with 32-channel spectral detector, Airy Scan, and Chameleon Discovery IR laser for 2-photon excitation

Zeiss 980 共焦显微镜，配备 32 通道光谱检测器、Airy Scan 和 Chameleon Discovery IR 激光器，用于 2 光子激发

基本信息

批准号：
10431118
负责人：
Anastas Popratiloff
金额：
$ 94.72万
依托单位：
GEORGE WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-07-20 至 2023-07-19
项目状态：
已结题

项目摘要

We request funds to purchase a Zeiss 980 confocal microscope equipped with a 32-channel linear array GaAsP photomultiplier spectral detector, Airyscan detector for high-resolution confocal imaging, visible laser launch, and Coherent Discovery (670-1330 nm) pulsed coherent light source for 2-photon excitation. The system will be equipped with an environmental chamber supporting live-cell experiments. The instrument will be programable to execute complex experimental sequences, capable of remote operations, collaborations, and multimodal image integration and will become a central piece of cross-technology workflows. The inherent ability of this instrument to generate large-format data will be addressed by two dedicated computers, synchronously managing the acquisition and processing of the data. The selected instrument will sustain and expand our current biomedical microimaging program following service termination of our workhorse spectral confocal – Zeiss 710. The system will serve a multitude of experiments, including live-cell imaging, photomanipulation, high-resolution large-area registrations, and large-volume registrations. The spectral capabilities of our Zeiss 710 have supported our research base to establish a robust biomedical microimaging program by eliminating uncertainties with fluorescence quality assurance, enabling imaging of cleared tissues and organisms presenting autofluorescence and tissues fixed with glutaraldehyde for correlative light-to-electron microscopy imaging. The proposed Zeiss 980 will empower us to continue in this direction while expanding our experimental portfolio offering also sub-diffraction microimaging - the standards of the next decade. Zeiss 980 will open doors for a more robust subcellular imaging, enhancing experiments focused on living and fixed cells and organisms, allowing discrete photomanipulation with visible and 2-photon excitation, functional imaging of NADH, label-free imaging of lipids, and second harmonic generation. The Airyscan will establish our portfolio in sub-diffraction resolution imaging of complex tissues, organs, and embryos, including optically cleared tissues and organs. This system will be exclusively available to the NIH-funded research community, with the major/minor users having access immediately after installation, but with the expectation to grow a user base, including newly NIH-funded investigators. This growth will follow a democratic principle involving the advisory committee. The well- established GW Nanofabrication and Imaging Center (GWNIC), which is the major technology resource for biomedical micro-and nanoimaging in the university, is supported by the GW’ Office of the Vice Provost for Research. GWNIC will provide administrative support, training of new users and will develop innovative approaches. Zeiss 980 will be a central component of cross-technology workflows such as correlative light- electron microscopy and will support our annual workshop. The instrument will be installed in GWNIC’s space within the School of Medicine and Health Sciences with the aim to effectively address the sample logistics and compliance by the biomedical segment of our research base.

我们要求资金购买配备32通道线性阵列的Zeiss 980共聚焦显微镜 GAASP光电倍增子谱检测器，用于高分辨率共聚焦成像的Airyscan检测器，可见激光发射和连贯的发现（670-1330 nm）脉冲相干光源，以进行2光孔兴奋。系统将配备支持活细胞实验的环境室。该仪器将是可以执行复杂的实验序列的程序，该序列能够进行远程操作，协作和多模式图像集成将成为跨技术工作流的中心部分。遗传性该仪器生成大幅面数据将由两台专用计算机解决，同步管理数据的获取和处理。选定的工具将维持和扩展我们的当前生物医学微成像计划在我们的士兵频谱共焦 - Zeiss 710的服务终止之后。该系统将提供多种实验，包括活细胞成像，光持续性，高分辨率大型注册和大容量注册。我们Zeiss 710的光谱功能具有支持我们的研究基础，以消除不确定性来建立强大的生物医学微参数计划具有荧光质量保证，可以对清除的组织和生物进行成像自动荧光和用戊二醛固定的组织，用于矫正灯到电子显微镜成像。这拟议的Zeiss 980将使我们能够继续朝这个方向继续，同时扩展我们的实验组合还提供小额分配微成像 - 未来十年的标准。 Zeiss 980将开门更健壮的亚细胞成像，增强的实验集中在生物和固定细胞和生物上，允许带有可见和2光子兴奋的离散光持持续的光持续，NADH的功能成像，无标签脂质的成像和第二次谐波产生。 Airyscan将以分类为单位建立我们的投资组合复杂组织，器官和胚胎的分辨率成像，包括光学清除的组织和器官。这 NIH资助的研究社区将专门提供系统，主要/次要用户拥有安装后立即访问，但期望增加用户群，包括新资助调查人员。这种增长将遵循咨询委员会的民主原则。福已建立的GW纳米制造和成像中心（GWNIC），这是主要技术资源该大学的生物医学微型和纳米影像受到GW副教务长的支持研究。 GWNIC将提供行政支持，对新用户的培训，并将开发创新方法。 Zeiss 980将是跨技术工作流的核心组成部分，例如相关光 - 电子显微镜将支持我们的年度研讨会。该乐器将安装在Gwnic的空间中在医学和健康科学学院内，旨在有效地解决样本登录和我们研究基础的生物医学段的遵守。