Proteomics Shared Resource

蛋白质组学共享资源

• 批准号: 10430007
• 负责人: TIMOTHY J EBERLEIN
• 金额: $ 27.69万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-02 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10430007
• 关键词: Acute Myelocytic Leukemia; Aftercare; Agreement; Algorithmic Analysis; Area; Basic Science; Bioinformatics; Biological Assay; Cancer Biology; Cancer Center; Cancer Center Support Grant; Cells; Clinical; Clinical Trials; Collaborations; Complex; Comprehensive Cancer Center; Computer software; Critiques; Custom; Data; Data Analyses; Data Discovery; Development; Enzymes; Equipment; Experimental Designs; Faculty; Funding; Future; Generations; Genes; Genome; Genomics; Goals; Guidelines; Human; Institutes; Label; Laboratories; Leadership; Libraries; Malignant Neoplasms; Malignant neoplasm of ovary; Mass Spectrum Analysis; Metabolic; Methods; NCI Center for Cancer Research; National Cancer Institute; North Carolina; Outcome; Peptide Sequence Determination; Peptides; Pharmaceutical Preparations; Phosphorylation; Phosphorylation Site; Phosphotransferases; Post-Translational Protein Processing; Protein Kinase; Proteins; Proteome; Proteomics; Proteomics Shared Resource; Protocols documentation; Publications; Reproducibility; Research; Resource Sharing; Sampling; Science; Services; Site; Specificity; Specimen; Stable Isotope Labeling; Standardization; Technology; Time; Translational Research; United States National Institutes of Health; Universities; Validation; Washington; Work; analysis pipeline; automated analysis; bioinformatics resource; bioinformatics tool; cancer type; clinical practice; cohort; computer cluster; computerized tools; data acquisition; density; design; experience; genome resource; improved; instrumentation; large scale data; medical schools; member; migration; multiplex assay; new technology; next generation; phosphoproteomics; programs; protein protein interaction; proteogenomics; recruit; repository; response; software development; tool; tumor

PROTEOMICS SHARED RESOURCE (PSR): PROJECT SUMMARY The Siteman Cancer Center (SCC) Proteomics Share Resource (PSR) has been a major contributor to the emerging field of proteogenomics with a track record of high impact publications and external funding. The PSR is poised to continue as leaders in the field in the next five years, using reproducible and repeatable deep-scale proteomics (≥ 10,000 genes) and phosphoproteomics (≥ 37,000 sites) and the harmonized protocol that was co- developed with the National Cancer Institute Clinical Proteomics Tumor Analysis Consortium (CPTAC). Studies are underway with comprehensively characterized Acute Myeloid Leukemia and pre/post treatment ovarian cancer cohorts (Aim 1). In the current project period, PSR has developed high-precision assays for absolute quantification (copies/cell) of 361 of the 526 protein kinases in the human kinome using CPTAC guidelines for validation toward FDA approval and clinical utility. The PSR proposes to have complete coverage of the human kinome by 2023 and to continue applying and developing cancer-type specific panels for PDX and human drug trials (Aim 2). Proteomics is on the cusp of next generation instrumentation that will lead to quantification of all expressed proteins and generate wider coverage of post-translational modifications. Using these new platforms, and in collaboration with the recently-established Washington University School of Medicine (WUSM) Mass Spectrometry Center, the PSR will develop new services for SCC members and increase the value and utility of proteogenomic analysis in support of SCC research programs (Aim 3). Future directions for the next project period include an emphasis on expanding services and enhancing cross-core interface to improve user experience and interpretation of data. PSR will continue to develop software in collaboration with the bioinformatic resources of the Genome Technology Access Center (GTAC) and The McDonnell Genome Institute (MGI) with the overarching goal of providing tools for more efficient interpretation of high-density proteogenomic data. WUSM recently recruited two faculty members who will contribute to PSR plans to expand services in the next project period. Ben Major (MCBP), from the Lineberger Comprehensive Cancer Center at the University of North Carolina, will serve as PSR Faculty Advisor and actualize expansion of PSR services in the area of proximity-labeling mass spectrometry for high-specificity characterization of intracellular complexes in cancer biology. With the successful recruitment of Dennis Goldfarb to WUSM and his agreement to collaborate on development of new MS and bioinformatics tools for proteogenomics, PSR will offer members new capabilities for facile discovery and hypothesis generation.

蛋白质组学共享资源（PSR）：项目摘要 现场癌症中心（SCC）蛋白质组学共享资源（PSR）一直是造成这种情况的主要因素 蛋白质组学的新兴领域具有高影响出版物和外部资金的记录。 PSR 在接下来的五年中，被毒死以继续担任该领域的领导者，使用可复制且可重复的深度尺度 蛋白质组学（≥10,000个基因）和磷酸蛋白质组学（≥37,000个位点）以及统一方案是共同的 由国家癌症研究所临床蛋白质组学分析联盟（CPTAC）开发。研究 正在进行全面特征的急性髓样白血病和治疗前卵巢 癌症队列（AIM 1）。在当前的项目期间，PSR已开发了高精度测定的绝对测定法 使用CPTAC指南的526个蛋白激酶的361定量（副本/细胞）使用CPTAC指南 验证FDA批准和临床实用程序。 PSR提议完全覆盖人 到2023年的Kinome，并继续应用和开发PDX和人类药物的癌症特异性面板 试验（目标2）。蛋白质组学是在下一代仪器的风口浪尖上，这将导致所有人的量化 表达蛋白质并产生更广泛的翻译后修饰范围。使用这些新平台， 并与最近建立的华盛顿大学医学院（WUSM）群众合作 光谱中心，PSR将为SCC成员开发新的服务，并增加的价值和实用性 支持SCC研究计划的蛋白质分析（AIM 3）。下一个项目的未来指示 期间包括强调扩展服务和增强跨核接口以改善用户 数据的经验和解释。 PSR将继续与 基因组技术访问中心（GTAC）和麦克唐纳基因组的生物信息学资源 研究所（MGI）的总体目标是提供工具，以更有效地解释高密度 蛋白质组数据。 WUSM最近招募了两名将为PSR计划做出贡献的教职员工 在下一个项目期间的服务。 Ben Major（MCBP），来自Lineberger综合癌症中心 北卡罗来纳大学将担任PSR教师顾问，并在 近端标记质谱的区域，用于高特异性的细胞内复合物 在癌症生物学中。随着丹尼斯·戈德法布（Dennis Goldfarb）的成功招募到WUSM及其合作的协议 关于用于蛋白质组学的新MS和生物信息学工具的开发，PSR将为会员提供新功能 用于轻松发现和假设产生。