Core A. Metabolomics and lipidomics

核心 A. 代谢组学和脂质组学

基本信息

批准号：
10426176
负责人：
Kyu Y Rhee
金额：
$ 24.47万
依托单位：
WEILL MEDICAL COLL OF CORNELL UNIV
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-06-12 至 2025-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10426176
关键词：
Biochemical Biochemical Reaction Biochemistry Bioinformatics Biological Assay Cells Collaborations Detection Disease Enzymes Gene Proteins Genes Genetic Engineering Goals In Vitro Individual Link Lipids Mass Spectrum Analysis Mediating Mediator of activation protein Metabolic Metabolism Methods Molecular Mycobacterium tuberculosis Nucleic Acids Nucleotides Pathway Analysis Pathway interactions Physiological Physiological Processes Physiology Proteins Proteome Proteomics Publishing Reagent Recombinants Research Project Grants Resolution Source Structure-Activity Relationship System Technology Tuberculosis Untranslated RNA base computerized data processing gene function genetic manipulation in vitro activity insight lipidome lipidomics metabolomics protein profiling protein structure function small molecule stressor tool

项目摘要

Core A, Abstract This technology core, Metabolomics and Lipidomics Core, aims to provide systems level biochemical insight into the function of key genes, proteins and non-coding RNAs required for adaptation of Mycobacterium tuberculosis (Mtb) to disease relevant stressors. To do so, it will make use of a suite of metabolomic technologies largely pioneered by this core and specifically developed for studies of Mtb physiology. Despite the widely recognized importance of metabolism as a quantitative source of biosynthetic precursors and energy, growing evidence has established its importance as an equally important and specific qualitative mediator of nearly all physiologic processes. Metabolomics is thus emerging as a window into systems level biochemistry. This core will specifically provide biologically unbiased metabo-lipidomic readouts of: (i) the in vitro activities of key genes, proteins and non-coding RNAs required for adaptation of Mycobacterium tuberculosis (Mtb) to disease relevant stressors; and (ii) the identities and activities of their physiologically linked pathways in wild type and genetically engineered Mtb strains. The Core’s Specific Aims are to: (1) Resolve the in vitro biochemical activities of purified recombinant Mtb proteins of unknown function or only class-level annotation; (2) Perform unbiased metabo-lipidomic comparisons of wild type and genetically manipulated Mtb strains; and (3) Perform unbiased activity-based metabolomic comparisons of Mtb proteomes.

核心A，抽象这种技术核心，代谢组学和脂肪组学核心旨在为系统水平的生化洞察力提供结核分枝杆菌适应所需的关键基因，蛋白质和非编码RNA的功能（MTB）与疾病相关的压力源。为此，它将在很大程度上使用一套代谢组技术由该核心开创，专门用于MTB生理学研究。尽管被广泛认可代谢作为生物合成前体和能量的定量来源的重要性，越来越多的证据已经具有确立了它的重要性，成为几乎所有生理学的同样重要和特定的定性调解人过程。因此，代谢组学是进入系统级生物化学的窗口。该核心将专门提供具有生物学上无偏的代谢 - 脂肪组读数：（i）适应结核分枝杆菌（MTB）所需的关键基因，蛋白质和非编码RNA需要疾病相关的压力源；（ii）它们在野外的身份和活动的身份和活动类型和一般设计的MTB菌株。核心的具体目的是：（1）解决体外生化纯化的重组MTB蛋白的活性，具有未知功能或仅类级注释的活性；（2）执行野生型和一般操纵的MTB菌株的无偏代代动物脂组学比较；（3）执行 MTB蛋白质组的无偏基基因代谢组比较。