Immunophenotypes in the Idiopathic Inflammatory Myopathies

特发性炎症性肌病的免疫表型

基本信息

批准号：
10427681
负责人：
Erin M Wilfong
金额：
$ 16.33万
依托单位：
VANDERBILT UNIVERSITY MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-07-26 至 2027-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10427681
关键词：
Academic Medical Centers Active Learning Affect Amino Acyl-tRNA Synthetases Autoantibodies Award B-Lymphocytes Bioinformatics Biology Biometry CD19 gene CXCR4 gene Cell physiology Cells Cellular Immunology Cellular Metabolic Process Cessation of life Characteristics Chemicals Clinical Communication Connective Tissue Diseases Critical Care Critical Illness Cytometry Data Data Set Development Development Plans Disease Doctor of Medicine Doctor of Philosophy Ensure Environment Equipment Fostering Foundations Funding Future Gene Expression Profile Genetic Transcription Goals Heterogeneity Human Hypersensitivity Idiopathic Inflammatory Myopathies Immune Immunologics Immunology Immunophenotyping Inflammatory Interstitial Lung Diseases Investigation K-Series Research Career Programs Knowledge Leadership Learning Lentivirus Lung Lung Transplantation Machine Learning Medicine Mentors Mentorship Metabolic Methodology Molecular Immunology Muscle Myositis Organ Outcome Oxidation-Reduction Pathogenesis Pathogenicity Pathway interactions Patients Pattern Peripheral Blood Mononuclear Cell Physicians Population Postdoctoral Fellow Principal Investigator Pulmonology Rare Diseases Research Project Grants Research Proposals Respiratory Failure Rheumatism Rheumatology Sampling Scientist Scleroderma Severity of illness Skin Syndrome T-Lymphocyte TXNIP gene Techniques Technology Time Training Transfection Up-Regulation analytical tool biobank career career development clinical phenotype cohort experience experimental study extracellular high dimensionality in vitro Assay inhibitor insight instructor longitudinal analysis metabolic profile monocyte neuromuscular new therapeutic target novel overexpression programmed cell death protein 1 programs research study sensor single-cell RNA sequencing skills supplemental oxygen transcriptomics translational research program

Academic Medical Centers Active Learning Affect Amino Acyl-tRNA Synthetases Autoantibodies Award B-Lymphocytes Bioinformatics Biology Biometry CD19 gene CXCR4 gene Cell physiology Cells Cellular Immunology Cellular Metabolic Process Cessation of life Characteristics Chemicals Clinical Communication Connective Tissue Diseases Critical Care Critical Illness Cytometry Data Data Set Development Development Plans Disease Doctor of Medicine Doctor of Philosophy Ensure Environment Equipment Fostering Foundations Funding Future Gene Expression Profile Genetic Transcription Goals Heterogeneity Human Hypersensitivity Idiopathic Inflammatory Myopathies Immune Immunologics Immunology Immunophenotyping Inflammatory Interstitial Lung Diseases Investigation K-Series Research Career Programs Knowledge Leadership Learning Lentivirus Lung Lung Transplantation Machine Learning Medicine Mentors Mentorship Metabolic Methodology Molecular Immunology Muscle Myositis Organ Outcome Oxidation-Reduction Pathogenesis Pathogenicity Pathway interactions Patients Pattern Peripheral Blood Mononuclear Cell Physicians Population Postdoctoral Fellow Principal Investigator Pulmonology Rare Diseases Research Project Grants Research Proposals Respiratory Failure Rheumatism Rheumatology Sampling Scientist Scleroderma Severity of illness Skin Syndrome T-Lymphocyte TXNIP gene Techniques Technology Time Training Transfection Up-Regulation analytical tool biobank career career development clinical phenotype cohort experience experimental study extracellular high dimensionality in vitro Assay inhibitor insight instructor longitudinal analysis metabolic profile monocyte neuromuscular new therapeutic target novel overexpression programmed cell death protein 1 programs research study sensor single-cell RNA sequencing skills supplemental oxygen transcriptomics translational research program

展开

项目摘要

PROJECT SUMMARY Candidate: Dr. Erin Wilfong, M.D., Ph.D. is a Clinical Instructor at Vanderbilt University Medical Center in the Divisions of Allergy, Pulmonary, & Critical Care Medicine and Rheumatology & Immunology. She has a strong clinical background in both rheumatology and pulmonology with an excellent scientific foundation in chemical biology and translational immunology from both her Ph.D. studies and her post-doctoral research studies following clinical training. Her long-term career plan is to become a leading physician-scientist in inflammatory myositis focused on the use of cutting-edge technologies to identify disease pathogenesis and novel therapeutic targets. To achieve this, her immediate goal is to (1) learn the requisite bioinformatic techniques to independently analyze high-dimensional immunophenotypic and transcriptional profiles and integrate these profiles with clinical outcomes, and (2) apply molecular immunology techniques to determine dysregulated pathways in IIM-specific cell populations. Research Project: This proposal will study the underlying immunologic heterogeneity of IIM and identify associations between immune/transcriptional signatures and clinical characteristics. Dr. Wilfong will (1) identify immune signatures and transcriptional aberrancies in IIM using a cross-sectional IIM cohort and (2) correlate immune features with ILD progression in a longitudinal IIM cohort. She will also take a prior single-cell RNA- Seq finding and (3) perform mechanistic studies investigate how upregulation of the redox sensor TXNIP alters immunometabolism in Jo1+ anti-tRNA synthetase syndrome. Career Development: Dr. Wilfong’s career development plan integrates formal coursework with personalized training from her mentors and collaborators to: (1) master R programming to facilitate analysis of immunophenotypic and transcriptional datasets using cutting-edge analytic tools, (2) learn biostatistical principles necessary to appropriately correlate immunology findings to clinical phenotypes, (3) characterize cellular function using in vitro assays, and (4) develop the leadership and communication skills necessary to become a principal investigator who applies the scientific findings and methodologies from this K08 award towards a future translational immunology R01. Environment: VUMC is the ideal environment to foster Dr. Wilfong’s development as a leader in translational immunology and inflammatory myositis. Dr. Wilfong’s mentoring team includes experts in inflammatory myositis (Crofford/Aggarwal), immunometabolism (Rathmell), single-cell RNA-Seq (Kropski), and computational immunology (Georgiev). In addition to having the necessary mentors and equipment, 71.3% of Vanderbilt University Medical Center career development award recipients (n=236) have successfully received R level funding since 1999. Drs. Crofford and Rathmell have successfully trained many physician-scientists and will ensure that Dr. Wilfong becomes an independently funded physician scientist.

项目摘要候选人：医学博士Erin Wilfong博士是范德比尔特大学医学中心的临床讲师过敏，肺和重症监护医学以及风湿病学和免疫学的分裂。她有很强的风湿病学和肺病学的临床背景，具有良好的化学科学基础她的博士学位的生物学和转化免疫学研究和她的博士后研究进行临床训练。她的长期职业计划是成为炎症领域的领先身体科学家肌炎的重点是使用尖端技术来鉴定疾病发病机理和新颖治疗靶标。为了实现这一目标，她的直接目标是（1）学习必要的生物信息学技术独立分析高维的免疫型和转录曲线，并将其整合具有临床结局的轮廓，（2）应用分子免疫学技术来确定失调 IIM特异性细胞种群中的途径。研究项目：该提案将研究IIM的潜在免疫异质性并确定免疫/转录特征与临床特征之间的关联。威尔冯博士（1）确定 IIM中使用横截面IIM队列和（2）相关的IIM中的免疫特征和转录异常在纵向IIM队列中具有ILD进展的免疫特征。她还将服用以前的单细胞RNA- SEQ发现和（3）进行机械研究研究氧化还原传感器TXNIP的上调如何改变 JO1+抗TRNA合成酶综合征中的免疫代谢。职业发展：威尔冯博士的职业发展计划将正式课程与个性化融合她的导师和合作者培训：（1）主编程，以促进分析使用尖端分析工具的免疫肾上腺素型和转录数据集，（2）学习生物统计学适当将免疫学发现与临床表型相关的原则，（3）特征是使用体外评估的细胞功能，以及（4）发展所需的领导和沟通技巧成为一名首席研究员迈向未来的翻译免疫学R01。环境：VUMC是培养威尔刚博士作为转化领域的发展的理想环境免疫学和炎症性肌炎。威尔冯博士的心理团队包括炎症专家肌炎（Crofford/Aggarwal），免疫代谢（Rathmell），单细胞RNA-Seq（Kropski）和计算免疫学（Georgiev）。除了拥有必要的问题和设备外，还有71.3％范德比尔特大学医学中心职业发展奖获得者（n = 236）已成功收到自1999年以来的R水平资金。 Crofford和Rathmell已成功培训了许多身体科学家并将确保Wilfong博士成为一名独立资助的物理科学家。