Caenorhabditis Genetics Center

秀丽隐杆线虫遗传学中心

基本信息

批准号：
10413373
负责人：
Aric L Daul
金额：
$ 45.53万
依托单位：
UNIVERSITY OF MINNESOTA
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-09-01 至 2027-02-28
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10413373
关键词：
Aging Animal Model Animals Anthelmintics Apoptosis Applied Research Area Basic Science Biological Biology Biomedical Research CRISPR/Cas technology Caenorhabditis Caenorhabditis elegans Cell Aging Chromosomal Rearrangement Collection Color Communities Country Cryopreservation Developmental Process Disease Education Educational process of instructing Ensure Equipment Fees Future Gene Proteins Genes Genetic Goals Growth Health Human In Vitro Individual International Laboratories Livestock Locales Location Measures MicroRNAs Microscopy Mission Modeling Monitor Nematoda Neurodegenerative Disorders Organism Strains Parasites Parasitic nematode Performance Persons Property Proteins Protocols documentation Publishing RNA Interference Reproducibility Research Research Personnel Research Project Grants Research Support Resources Safety Scheme Science Scientist Services Signal Transduction System Techniques Training Work animal resource cost design gene induction in vivo in vivo evaluation insight member mutant null mutation preservation repository research study tool tumor progression

Aging Animal Model Animals Anthelmintics Apoptosis Applied Research Area Basic Science Biological Biology Biomedical Research CRISPR/Cas technology Caenorhabditis Caenorhabditis elegans Cell Aging Chromosomal Rearrangement Collection Color Communities Country Cryopreservation Developmental Process Disease Education Educational process of instructing Ensure Equipment Fees Future Gene Proteins Genes Genetic Goals Growth Health Human In Vitro Individual International Laboratories Livestock Locales Location Measures MicroRNAs Microscopy Mission Modeling Monitor Nematoda Neurodegenerative Disorders Organism Strains Parasites Parasitic nematode Performance Persons Property Proteins Protocols documentation Publishing RNA Interference Reproducibility Research Research Personnel Research Project Grants Research Support Resources Safety Scheme Science Scientist Services Signal Transduction System Techniques Training Work animal resource cost design gene induction in vivo in vivo evaluation insight member mutant null mutation preservation repository research study tool tumor progression

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项目摘要

Project Summary/Abstract—Overall The Caenorhabditis Genetics Center (CGC) is the sole comprehensive repository and distribution center for the nematode Caenorhabditis elegans, a premier model organism for biomedical research studies. The overall objective of this animal resource is to promote research on C. elegans by acquiring, maintaining, and distributing genetically characterized nematode stocks. Researchers in all 50 states and throughout the world use genetic stocks obtained from the CGC in diverse basic and applied research endeavors, as well as for hands-on teaching of experimental science. Studies using C. elegans have led to fundamental insights into basic biological mechanisms, including the genetic basis of programmed cell death, the discovery of microRNAs, and the mechanism of RNA interference in animals. The nematode has also provided key insights into the cell signaling mechanisms, cancer progression, and neurodegenerative diseases. In addition, C. elegans serves as a key model for illuminating our understanding of parasitic nematodes with relevance to human and livestock health. As the only general stock center for C. elegans, the CGC is an extremely important international research resource, supporting research in these diverse areas and in educational endeavors. The CGC provides about 30,000 strains per year to thousands of laboratories; with a collection of 23,000 unique strains, a number still increasing in proportion to the growth of the field, the CGC not only facilitates research, but also ensures that valuable strains are preserved. Strains are distributed upon request through an on-line ordering system with a scheme of user fees in place to help defray costs and support CGC activities. The CGC closely monitors user needs and devises small research projects to support the community. Because essentially every lab uses fluorescent proteins, and there is demand for brighter, more photostable fluorescent proteins, in more colors to allow multi-plexing, the CGC will develop a pipeline to quantitatively evaluate the in vivo properties of a new set of fluorescent proteins in worm, enabling scientists to choose the optimal versions for their applications.

项目摘要/摘要 - 综合 Caenorhabditis遗传学中心（CGC）是唯一的全面存储库和配送中心线虫Caenorhabditis秀丽隐杆线虫，一种用于生物医学研究的主要模型生物。总体该动物资源的目的是通过获取，维护和分发来促进秀丽隐杆线虫的研究遗传表征的线虫库存。所有50个州和世界各地的研究人员都使用遗传从CGC获得的股票在潜水员基本和应用研究的努力以及动手教学方面获得的股票实验科学。使用秀丽隐杆线虫的研究导致对基本生物学的基本见解机制，包括编程细胞死亡的遗传基础，发现microRNA和 RNA干扰动物的机制。线虫还提供了对单元信号传导的关键见解机制，癌症进展和神经退行性疾病。此外，秀丽隐杆线虫还充当钥匙阐明我们对寄生线虫的理解与人类和牲畜健康相关的模型。作为秀丽隐杆线虫的唯一一般股票中心，CGC是一项极为重要的国际研究资源，支持这些潜水区和教育努力的研究。 CGC提供每年为成千上万的实验室应变；收集了23,000个独特的菌株，仍然有一个 CGC与该领域的增长成比例，不仅有助于研究，而且还确保了保留了有价值的应变。应要求通过在线订购系统中分配菌株制定的用户费用方案，以帮助支付成本并支持CGC活动。 CGC密切监视用户需要并设计小型研究项目来支持社区。因为本质上每个实验室都使用荧光蛋白质，并且需要更明亮，更稳定的荧光蛋白，以更多的颜色到允许多重复杂，CGC将开发一条管道，以定量评估新集合的体内特性蠕虫中的荧光蛋白，使科学家可以为其应用选择最佳版本。