Molecular Biology and Cytometry Research Shared Resource

分子生物学和细胞计数研究共享资源

基本信息

批准号：
10413074
负责人：
Jones Kenneth
金额：
$ 14.36万
依托单位：
UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-05-01 至 2023-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10413074
关键词：
Area Biological Assay Biomedical Research Cancer Center Cancer Center Support Grant Cell Cycle Cells Complex Consultations Core Facility Cytometry DNA sequencing Data Data Collection Disease Doctor of Philosophy Electrophoresis Experimental Designs Fees and Charges Floor Flow Cytometry Gel Genes Genomics Goals Image Infrastructure Laboratories Location Malignant Neoplasms Mass Spectrum Analysis Measurement Metabolic Microscopy Molecular Biology Molecular Weight Nucleic Acids Nucleic acid sequencing Peer Review Peptides Post-Translational Protein Processing Preparation Proteins Proteomics Protocols documentation Research Research Personnel Resolution Resource Sharing Sampling Science Services Signal Transduction Site Technology Training base cancer cell functional genomics high throughput screening high throughput technology incentive program instrumentation liquid chromatography mass spectrometry member multimodality nano next generation protein expression screening searchable database service member

Area Biological Assay Biomedical Research Cancer Center Cancer Center Support Grant Cell Cycle Cells Complex Consultations Core Facility Cytometry DNA sequencing Data Data Collection Disease Doctor of Philosophy Electrophoresis Experimental Designs Fees and Charges Floor Flow Cytometry Gel Genes Genomics Goals Image Infrastructure Laboratories Location Malignant Neoplasms Mass Spectrum Analysis Measurement Metabolic Microscopy Molecular Biology Molecular Weight Nucleic Acids Nucleic acid sequencing Peer Review Peptides Post-Translational Protein Processing Preparation Proteins Proteomics Protocols documentation Research Research Personnel Resolution Resource Sharing Sampling Science Services Signal Transduction Site Technology Training base cancer cell functional genomics high throughput screening high throughput technology incentive program instrumentation liquid chromatography mass spectrometry member multimodality nano next generation protein expression screening searchable database service member

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项目摘要

MOLECULAR BIOLOGY AND CYTOMETRY RESEARCH SHARED RESOURCE – ABSTRACT The Molecular Biology and Cytometry Research Shared Resource (MBCR SR) is an institutionally managed central core facility that assists investigators in all aspects of research, including initial project consultation, assistance with experimental design, training in protocols and instrumentation usage, sample preparation, data collection and assistance with analysis and interpretation of results. Under the direction of James Tomasek, PhD, the OUHSC Vice President for Research, the MBCR SR provides four important Core services to SCC members and other investigators: 1) the DNA Sequencing and Genomics Core provides investigators with access to high-fidelity, next-generation nucleic acid sequencing data; 2) the Mass Spectrometry and Proteomics Core provides proteomics support for biomolecule identification, including complex proteomics analysis, post-translational modification identification, high-resolution molecular weight determination, large biomolecular mass measurements, LC-UV-MS analysis (qualitative or quantitative), protein identification by proteolytic digest (in-gel or in solution), nano LC/MS/MS and database search, and peptide sequencing; 3) the Flow Cytometry and Imaging Core provides flow cytometry and microscopy support for subcellular studies on protein expression, cell cycle and cell signaling; and 4) the Cancer Functional Genomics Core provides cutting- edge technology and services for high-throughput live cell functional characterization of cancer cells, including reverse proteomics array establishment, absolute gene quantification and copy number determination, nucleic acid and protein electrophoresis and imaging, multimodal screening for high-throughput assay on plates, high- throughput, high-content screening of subcellular localized proteins in cells, metabolic analysis of live cells and quantification of clonogenic assay colonies of cancer cells. The MBCR SR occupies a total of 6,409 sf. of laboratory space on the 1st, 3rd and 4th floors in the Stanton L. Young Biomedical Research Center (BRC) facility on the OUHSC campus. This central location provides the 27 BRC-based SCC members with immediate access to this important infrastructure. An additional five SCC members are located in the adjacent Biomedical Sciences Building (BMSB), which has a dedicated internal walkway to BRC. In 2016, 22% of the total number of MBCR SR users were SCC members with peer-reviewed support. The facility also served 12 SCC members without peer-reviewed support. In 2016, SCC members constituted 32% of the total users of this centralized institutional facility, which is by far the largest usage by a department or disease-site group at OUHSC, highlighting the high utilization and impact that the facility has on advancing research at the SCC. An incentive program initiated by the SCC covers 20% of all charged fees for SCC members for services provided by all areas of the MBCR.

分子生物学和细胞术研究共享资源 - 摘要分子生物学和细胞术研究共享资源（MBCR SR）是由机构管理的中央核心设施，协助研究人员参与研究的各个方面，包括初步项目咨询，协助实验设计，协议和仪器使用培训，样品制备，数据收集和协助，分析和解释结果。在詹姆斯·托马塞克（James Tomasek）的指导下 OUHSC研究副总裁博士，MBCR SR为SCC提供了四个重要的核心服务成员和其他研究人员：1）DNA测序和基因组学核心为研究人员提供了获取高保真，下一代核酸测序数据； 2）质谱法和蛋白质组学核心为生物分子鉴定提供蛋白质组学支持，包括复杂的蛋白质组学分析，翻译后修饰鉴定，高分辨率分子量测定，大型生物分子质量测量，LC-UV-MS分析（定性或定量），蛋白质鉴定蛋白水解摘要（凝胶或溶液中），纳米LC/MS/MS和数据库搜索以及胡椒测序； 3）流式细胞仪和成像核心提供流式细胞术和显微镜支持，以支持对上细胞的研究蛋白质表达，细胞周期和细胞信号传导； 4）癌症功能基因组学核心提供了剪切边缘技术和服务，用于癌细胞的高通量实时细胞功能表征，包括反向蛋白质组学阵列建立，绝对基因定量和拷贝数确定，核心酸和蛋白质电泳和成像，对板上高通量测定的多模式筛选，高 - 细胞中亚细胞局部蛋白的吞吐量，高含量筛查，生活细胞的代谢分析和定量癌细胞的粘性测定菌落。 MBCR SR总共占6,409平方英尺。 Stanton L.一楼，三楼和4层的实验室空间。 OUHSC校园的年轻生物医学研究中心（BRC）设施。这个中心位置提供了 27位基于BRC的SCC成员，可以立即访问这一重要基础架构。另外五个SCC 成员位于相邻的生物医学大楼（BMSB）中，该大楼具有专用的内部到BRC的人行道。 2016年，MBCR SR用户总数的22％是SCC成员，并进行了同行评审支持。该设施还为12名SCC成员提供了服务，没有同行评审的支持。 2016年，SCC成员该集中式机构设施的总用户占32％，这是迄今为止最大的用法 OUHSC的部门或疾病组，强调了该设施对在SCC上进行研究。 SCC发起的激励计划涵盖了所有收费费的20％ SCC成员为MBCR所有领域提供的服务。