Animal Physiology Core

动物生理学核心

• 批准号: 10407756
• 负责人: LAURIE J GOODYEAR
• 金额: $ 24.27万
• 依托单位: JOSLIN DIABETES CENTER
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-02-15 至 2027-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10407756
• 关键词: Animal Model; Animals; Area; Biometry; Blood Glucose; Blood Pressure; Blood flow; Body Composition; Bone Density; Cells; Cellular Metabolic Process; Collection; Consult; Controlled Study; Data Collection; Diabetes Mellitus; Diabetic mouse; Disease Progression; Drug or chemical Tissue Distribution; Dual-Energy X-Ray Absorptiometry; Energy Metabolism; Equipment; Evaluation; Exercise; Exposure to; Feces; Functional disorder; Gene Expression Profile; Genetic; Genetically Engineered Mouse; Genomics; Germ-Free; Gnotobiotic; Hand Strength; Home; Human; Human Resources; Hyperoxia; Hypoxia; Imaging technology; Incubators; Insulin Resistance; Journals; Lasers; Martes zibellina; Measurement; Measures; Medicine; Metabolic; Metabolic Diseases; Metabolism; Molecular; Monitor; Mus; Nature; Obesity; Paper; Phenotype; Physiological; Physiology; Prevention; Procedures; Publications; Publishing; Rattus; Reporter; Research; Research Design; Research Personnel; Rodent; Role; Services; System; Tail; Temperature; Testing; Therapeutic Intervention; Training; Translational Research; Translations; Urine; Water consumption; animal imaging; base; clinical investigation; food consumption; glucose tolerance; improved; in vivo; in vivo Model; in vivo imaging system; insight; insulin tolerance; investigator training; meter; microbiome; microscopic imaging; mouse model; muscle strength; novel; optical imaging; perfusion imaging; pre-clinical; retinal imaging; trafficking; translational medicine; treadmill

ANIMAL PHYSIOLOGY CORE: Abstract The purpose of the Joslin Diabetes Research Center (DRC) Animal Physiology Core is to provide technically advanced physiological evaluation of rodents for the study of diabetes, obesity, and their associated complications. These studies provide a critical platform for extension of molecular discoveries to in vivo settings that provide critical insights for relevance to human metabolic diseases. The study of animal models is an integral part of Joslin Research. In addition to study of rats and established mouse models, Joslin DRC investigators have generated new strains of genetically engineered mice, the majority of which have been studied using the Animal Physiology Core. Services provided by the Animal Physiology enable investigators to quantify in vivo energy expenditure, activity and fuel utilization; body composition and bone density; arterial blood pressure and blood glucose levels; and hypoxia and hyperoxia. Equipment and expertise are available for in vivo and longitudinal animal imaging; controlled exposure to cold and thermoneutral temperatures using diurnal incubators; exercise using a rodent treadmill and activity wheel cages; assessment of muscle strength; and advanced retinal imaging and function. In addition, the Animal Physiology Core provides a Gnotobiotic Mouse Facility to study the role of the microbiome in metabolic diseases. Based on technological advances and needs of the DRC research base, the current application proposes substantial advances in the capabilities for quantification of energy expenditure and fuel utilization enabled by recent acquisition of a state-of the-art Sable Promethion System. The Animal Physiology Core provided services to 19 Joslin DRC Investigators and 11 outside Investigators in the past cycle. In addition, the Core educated investigators and fellows on the theoretical understanding of core procedures and data collection, consulted on study design, and provided hands-on training for some procedures and equipment. The Core has been essential in the publication of numerous papers, including many published in high impact journals such as Cell Metabolism, Nature Medicine, Nature Metabolism, Journal of Clinical Investigation, and Science Translational Medicine. Both institutional and DRC support for the Animal Physiology Core are substantial and enable Joslin’s Research Base to make physiologically relevant discoveries with potential for translation to improved treatments, prevention, and ultimately, cures for diabetes and related metabolic disorders.

动物生理核心：摘要 乔斯林糖尿病研究中心（DRC）动物生理核心的目的是在技术上提供 啮齿动物的高级物理评估，用于研究糖尿病，肥胖症及其相关的啮齿动物 并发症。这些研究为将分子发现扩展到体内设置提供了关键的平台 这为与人类代谢疾病相关的关键见解提供了关键的见解。动物模型的研究是不可或缺的 乔斯林研究的一部分。除了研究大鼠和已建立的小鼠模型外，Joslin DRC研究者 已经产生了一般工程小鼠的新菌株，其中大多数是使用了 动物生理核心。 动物生理提供的服务使研究人员能够量化体内能量消耗，活动 和燃料利用；身体成分和骨密度；动脉血压和血糖水平；和 缺氧和高氧。设备和专业知识可用于体内和纵向动物成像； 使用昼夜孵化器控制暴露于寒冷和热中性温度；使用啮齿动物锻炼 跑步机和活动轮笼；评估肌肉力量；和高级视网膜成像和功能。 此外，动物生理核心提供了一种gnotobiotic小鼠的设施来研究微生物组的作用 在代谢疾病中。基于刚果民主共和国研究基础的技术进步和需求，当前 申请提案在量化能源消耗和燃料的能力方面取得了重大进展 通过最近获得的最先进的黑貂求职系统来实现利用。 动物生理核心为19名Joslin DRC调查人员和11位外部调查人员提供服务 过去的周期。此外，核心教育的调查人员和研究员对核心的理论理解 程序和数据收集，对研究设计进行了咨询，并为某些程序提供了动手培训 和设备。核心对于发表众多论文至关重要，包括许多发表的论文 在高影响期刊中，例如细胞代谢，自然医学，自然代谢，临床杂志 调查和科学转化医学。机构和DRC对动物生理的支持 核心是实质性的，并且使乔斯林的研究基础能够与 改善治疗，预防以及最终治愈糖尿病和相关治疗的潜力 代谢障碍。