Stanford Islet Research Core

斯坦福岛研究核心

• 批准号: 10407863
• 负责人: Seung K Kim
• 金额: $ 21.5万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-15 至 2027-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10407863
• 关键词: Abbreviations; Aging; Alberta province; Area; Award; Biological Assay; Biology; Blood Cells; Blood Vessels; Body mass index; California; Canada; Cell Therapy; Cell physiology; Cells; Child Health; Clinical; Communities; Complex; Coupled; Diabetes Mellitus; Ensure; Enzyme-Linked Immunosorbent Assay; Epigenetic Process; Evolution; Functional disorder; Gene Expression Regulation; Genetic; Genome; Genomics; Glucagon; Growth; Healthcare; Hematopoietic stem cells; Human; Human Resources; Immune; Immune Tolerance; Immunocompromised Host; Immunologic Monitoring; Immunologics; Immunology; Institutes; Insulin; Insulin-Dependent Diabetes Mellitus; Investigation; Islet Cell; Islets of Langerhans; Islets of Langerhans Transplantation; Letters; Maintenance; Malignant neoplasm of pancreas; Methods; Modeling; Modernization; Molecular; Mus; Names; National Institute of Diabetes and Digestive and Kidney Diseases; Natural regeneration; Non-Insulin-Dependent Diabetes Mellitus; Organ; Organ Procurements; Pancreas; Pathologic; Phenotype; Physiological; Procedures; Publishing; Regulatory T-Lymphocyte; Research; Research Institute; Research Personnel; Rodent; San Francisco; Services; Signal Transduction; Training; Transplantation; Universities; Virginia; Work; base; chronic pancreatitis; endocrine pancreas development; experimental study; falls; human tissue; insulin secretion; investigator training; islet; medical schools; member; novel strategies; programs; ranpirnase; success; translational potential; translational study

STANFORD ISLET RESEARCH CORE (SIRC): PROJECT SUMMARY/ABSTRACT The specialized expertise of the SDRC Islet Research Core (SIRC) provides SDRC investigators with the capacity to perform modern molecular, cellular and functional studies of high-quality islets and pancreata from rodents and humans. The SDRC’s large group of collaborative investigators study a broad spectrum of islet biology in physiological or pathological settings, including islet development, functional maturation, maintenance of mature cell fate, proliferation, genetics, epigenetics, gene regulation, inter-organ signaling, intra-islet cell signal transduction, islet immunology, and aging. A central aspect of the SIRC is our direct focus on parallel studies in rodent and human tissues. An essential component supporting the majority of studies falling under this paradigm is our ability to isolate high-quality, well characterized rodent or human primary pancreatic islets, and then to perform assays of islet cellular, molecular and physiological phenotypes. This is coupled with a reliable, robust program to procure high-quality human islets that have increased the number of SDRC investigators studying human islet biology. The support of transplantation-based studies of human islet cell function, growth and fate by the SIRC also enhances potential translational studies of human islets for diabetes. In addition to these services, the SIRC is committed to training investigators in specialized methods of islet biology, like islet isolation and transplantation, islet culture and specialized assays including insulin or glucagon ELISA, islet perifusion and static batch insulin secretion assays, and immunohistology. SIRC personnel work closely with SDRC investigators to build efficient experimental strategies tailored to their aims. These islet-focused services or activities would be lost without P30 support of this unique Stanford research core. The SIRC also serves the Stanford community and the SDRC by continuously developing new experimental capacity to match the dynamic demands of modern islet investigations. This includes assays of islet function, new approaches to investigate human islet cell genetics, new models of islet transplantation, and assistance in developing new clinical islet programs at Stanford Health Care, like the Stanford Pancreatic Islet Replacement and Immune Tolerance (SPIRIT) clinical islet transplantation programs. The SIRC also enhances the use of other SDRC Research Cores by integrating activities with those cores. Together with the Diabetes Immune Monitoring Core (DIMC) and the Diabetes Genomics & Analysis Core (DGAC), the SIRC collaborates on efforts permitting cellular, genetic, molecular, physiological and genome studies of human pancreas- and immune or blood cells, including immune, stromal and vascular cells. The SIRC will also serve SDRC members at University of California (UC) Berkeley or at UC Davis, including those supported through the proposed Regional Pilot & Feasibility Award expansion. Based on growth of the SDRC membership, evolution of exciting new services, increasing membership demand for human islets, and the increased research focused on islet biology in the SDRC, we anticipate growth of demand for SIRC services in the coming years.

斯坦福大学研究核心（SIRC）：项目摘要/摘要 SDRC胰岛研究核心（SIRC）的专业专业知识为SDRC调查人员提供了 对高质量胰岛和胰腺的现代分子，细胞和功能研究 啮齿动物和人类。 SDRC的大批合作研究人员正在研究一系列胰岛 物理或病理环境中的生物学，包括胰岛发育，功能成熟， 维持成熟的细胞脂肪，增殖，遗传学，表观遗传学，基因调节，器官间信号传导， ISLEL内细胞信号翻译，胰岛免疫学和衰老。 SIRC的主要方面是我们的直接重点 关于啮齿动物和人体组织的平行研究。支持大多数研究的重要组成部分 属于这种范式是我们分离高质量，特征啮齿动物或人类原理的能力 胰岛，然后进行胰岛细胞，分子和物理表型的测定。这是 再加上可靠，健壮的计划，以获取增加数量增加数量的高质量人类胰岛 研究人类胰岛生物学的SDRC研究人员。基于移植的人类胰岛研究的支持 SIRC的细胞功能，生长和命运还增强了人类胰岛的潜在翻译研究 糖尿病。除这些服务外，SIRC还致力于以专业方法的培训调查人员 胰岛生物学，例如胰岛隔离和移植，胰岛培养和专业测定法，包括胰岛素或 胰高血糖素ELISA，胰岛渗透和静态批处理胰岛素分泌测定和免疫组织学。 sirc 人员与SDRC调查人员紧密合作，以建立针对其目标量身定制的有效实验策略。 如果没有P30支持这项独特的斯坦福大学研究，这些以小便为中心的服务或活动将丢失 核。 SIRC还通过不断开发新的新服务为Stanford社区和SDRC服务 与现代胰岛调查的动态需求相匹配的实验能力。这包括 胰岛功能，研究人类胰岛细胞遗传学的新方法，胰岛移植的新模型和 协助在斯坦福医疗保健中开发新的临床胰岛计划，例如斯坦福胰岛 替代和免疫耐受性（精神）临床胰岛移植计划。 SIRC还增强了 通过将活动与这些核心整合在一起，使用其他SDRC研究核心。与糖尿病一起 免疫监测核心（DIMC）和糖尿病基因组学与分析核心（DGAC），SIRC合作 允许人类胰腺和人类胰腺和基因组研究的细胞，遗传，分子，物理和基因组研究 免疫或血细胞，包括免疫，基质和血管细胞。 SIRC也将服务于SDRC 加州大学伯克利分校或加州大学戴维斯分校的成员，包括通过 拟议的区域飞行员和可行性奖扩展。基于SDRC成员资格的增长 令人兴奋的新服务，对人类胰岛的会员需求不断增长以及增加的研究 专注于SDRC中的胰岛生物学，我们预计未来几年对SIRC服务的需求会增长。