Decay Accelerating Factor and B cell Immunity

衰变加速因子和 B 细胞免疫

基本信息

批准号：
10406250
负责人：
David Dominguez-Sola
金额：
$ 42.38万
依托单位：
ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-06-17 至 2024-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10406250
关键词：
1-Phosphatidylinositol 3-Kinase Affinity Alternative Complement Pathway Antibodies Antibody Affinity Antibody Formation Antibody Response Antibody titer measurement Antigens Autoimmune Autoimmunity B Cell Proliferation B-Cell Activation B-Lymphocytes BCL6 gene Biological Biology C3AR1 gene C5a anaphylatoxin receptor CD55 Antigens Cell Survival Cell surface Cellular biology Chromosome 1 Complement Complement 3 Convertase Complement 3a Complement 3d Receptors Complement 5a Complement Activation Data Development Disease Down-Regulation Enhancers Event Follicular Dendritic Cells Future Generations Genes Genetic Transcription Humoral Immunities Immune response Immunity Immunization Immunoglobulin Somatic Hypermutation Impairment Infection Lifting Ligation Light Link Mediating Mediator of activation protein Memory B-Lymphocyte Modeling Molecular Mus Outcome Output Pathogenicity Physiological Plasma Cells Process Production Reaction Reagent Receptor Signaling Regulation Repression Research Role Signal Pathway Signal Transduction Solid Structure of germinal center of lymph node System T-Lymphocyte Testing Transgenic Organisms Transplantation Vaccines chronic graft versus host disease complement C3d,g complement pathway gene repression genetic regulatory protein in vivo insight lymphoid structures member novel organ transplant rejection overexpression pathogen prevent programs promoter receptor response restraint vaccine strategy

1-Phosphatidylinositol 3-Kinase Affinity Alternative Complement Pathway Antibodies Antibody Affinity Antibody Formation Antibody Response Antibody titer measurement Antigens Autoimmune Autoimmunity B Cell Proliferation B-Cell Activation B-Lymphocytes BCL6 gene Biological Biology C3AR1 gene C5a anaphylatoxin receptor CD55 Antigens Cell Survival Cell surface Cellular biology Chromosome 1 Complement Complement 3 Convertase Complement 3a Complement 3d Receptors Complement 5a Complement Activation Data Development Disease Down-Regulation Enhancers Event Follicular Dendritic Cells Future Generations Genes Genetic Transcription Humoral Immunities Immune response Immunity Immunization Immunoglobulin Somatic Hypermutation Impairment Infection Lifting Ligation Light Link Mediating Mediator of activation protein Memory B-Lymphocyte Modeling Molecular Mus Outcome Output Pathogenicity Physiological Plasma Cells Process Production Reaction Reagent Receptor Signaling Regulation Repression Research Role Signal Pathway Signal Transduction Solid Structure of germinal center of lymph node System T-Lymphocyte Testing Transgenic Organisms Transplantation Vaccines chronic graft versus host disease complement C3d,g complement pathway gene repression genetic regulatory protein in vivo insight lymphoid structures member novel organ transplant rejection overexpression pathogen prevent programs promoter receptor response restraint vaccine strategy

展开

项目摘要

Project Summary Humoral immunity crucially protects against pathogens but can function as a pathogenic mediator of multiple autoimmune and alloimmune disease processes, the latter including chronic graft vs. host disease and antibody-mediated solid organ transplant rejection. Development of antigen-specific antibody responses requires T cell-dependent B cell activation and the formation of germinal centers (GC), transient lymphoid structures where somatic hypermutation and affinity maturation events result in the generation of high affinity, antibody-producing plasma cells (PC) and memory B cells (Bmem). The physiological signals that control GC formation and GC B cell fates are incompletely characterized. Our preliminary data identify a previously unappreciated role for decay accelerating factor (DAF or CD55), a cell surface-expressed complement regulatory protein, in controlling GC reactions and their resultant output, the production of PC and Bmem. Our data support the hypothesis that optimal GC function requires downregulation of cell surface expressed DAF on responding B cells, a process that lifts restraint on local, alternative pathway complement activation and facilitates C3- and C5-convertase formation and production of C3 and C5 cleavage products. This process promotes C3a/C3aR1 and C5a/C5aR1 ligations on GC B cells, which would in turn transduce signals controlling GC B cell proliferation, survival, somatic hypermutation, and/or affinity maturation, and as a consequence, influence differentiation into memory B cells and/or plasma cells. We will test this paradigm- shifting hypothesis using unique biological reagents and through 3 interactive aims: 1) To determine the effects of B cell-expressed DAF, C3aR1 and C5aR1 on T cell-dependent humoral immune responses and distinguish them from the effects of B cell-expressed CD21. 2) To determine the cellular and molecular mechanisms through which DAF downregulation and enhanced signaling through C3aR1/C5aR1 in B cells drive affinity maturation. 3) To determine the molecular basis of DAF downregulation on B cells. This comprehensive analysis performed by a multi-PI team with expertise in complement biology (Heeger) and B cell biology (Dominguez-Sola) is likely to provide new insight into fundamental mechanisms of GC dynamics and function. The results have the potential to guide second order studies aimed at exploiting the complement/B cell axis to either inhibit development of pathogenic B cell responses (e.g. in transplantation or autoimmunity) or augment B cell response (e.g. in response to vaccines).

项目摘要体液免疫力至关重要地预防病原体，但可以充当多重病原体的介体自身免疫性和同种免疫性疾病过程，后者包括慢性移植与宿主疾病和抗体介导的固体器官移植排斥。抗原特异性抗体反应的发展需要T细胞依赖性B细胞激活和生发中心（GC）的形成，瞬态淋巴样体细胞超成熟和亲和力成熟事件导致高亲和力产生的结构，产生抗体的浆细胞（PC）和记忆B细胞（BMEM）。控制GC的生理信号形成和GC B细胞命运未完全表征。我们的初步数据确定了先前的衰减加速因子（DAF或CD55）的未欣赏角色，一种细胞表面表达的补体调节蛋白在控制GC反应及其产量时，PC和BMEM的产生。我们的数据支持最佳GC功能需要下调细胞表面表达DAF的假设在响应B细胞时，该过程可以限制局部替代途径补充激活和促进C3-和C5-转化酶形成以及C3和C5裂解产品的产生。这个过程在GC B细胞上促进C3A/C3AR1和C5A/C5AR1绑扎，这又会传输信号控制GC B细胞增殖，存活，体细胞超成名和/或亲和力成熟，并作为A 结果，影响分化为记忆B细胞和/或浆细胞。我们将测试此范式 - 使用独特的生物试剂转移假设，并通过3个交互式目的：1）确定影响 B细胞表达的DAF，C3AR1和C5AR1在T细胞依赖性体液免疫反应上的它们来自B细胞表达的CD21的影响。 2）确定细胞和分子机制通过在B细胞中通过C3AR1/C5AR1通过C3AR1/C5AR1的DAF下调和增强的信号传导驱动亲和力成熟。 3）确定B细胞上DAF下调的分子基础。这个全面由具有补体生物学专业知识（HEEGER）和B细胞生物学专业知识的多PI团队进行的分析（Dominguez-Sola）可能会提供有关GC动力学和功能基本机制的新见解。结果有可能指导旨在利用补体/B细胞轴的二阶研究抑制致病性B细胞反应的发展（例如，移植或自身免疫性）或增强 B细胞反应（例如，对疫苗的响应）。