Research Supplement for Kennelia Mellanson

肯尼莉亚·梅兰森的研究增刊

• 批准号: 10382622
• 负责人: Helene D Benveniste
• 金额: $ 5.71万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-15 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10382622
• 关键词: Age; Aging; Alzheimer' s Disease; Animal Model; Brain; Brain Mass; Cerebral Ventricles; Cerebrospinal Fluid; Complex; Development; Fostering; Grant; Homeostasis; Laboratories; Manuscripts; Mentorship; Nervous system structure; Research; Research Personnel; Structure; System; Techniques; TimeLine; Ventricular; Writing; aquaporin 4; brain health; brain tissue; career; cerebrospinal fluid flow; glymphatic system; graduate student; improved; meetings; mouse model; novel; response; skills; solute; ventricular system; wasting

ABSTRACT Here we apply for a research supplement to our R01 grant from the NIA, submitting it in response to PA-21-071 which is directed at fostering diversity in scientific research workforce and developing the careers of eligible investigators. We present a detailed plan to advance the professional career of Kennelia Mellanson, a graduate student in the laboratory. Kennelia’s current project focuses on novel quantitative approaches to determine the key components of the ventricular and glymphatic system of transport in the brain – the ciliary beat, the directional flow of the cerebrospinal fluid (CSF), and the structure of the brain ependymal layer – features that are critical for the efficient ventricular transport of the CSF, and the structure of the aquaporin 4 (AQP4) channels, which serve as the key components of the glymphatic system of transport. Our proposal is in full alignment with the original R01 grant from the NIA “Glymphatic and CSF systems in aging and Alzheimer’s disease”, which focuses on the mechanisms of the brain tissue homeostasis and clearance in Alzheimer's. We now propose using our new techniques and findings on the ventricular and glymphatic transport to determine the changes accompany aging and progression of the Alzheimer's disease in a mouse model and to use this project as a platform for helping and directing Kennelia Mellanson in developing her career as a researcher and a scholar. We present a detailed plan for Kennelia’s professional development, including a detailed plan and timeline for mentorship, manuscript and grant writing, didactic activities, improving the presentation skills, participation in meetings and courses, and networking. Furthermore, we present a research plan for studying the key components of the transport systems that may be compromised by aging and by Alzheimer's disease.

抽象的 在此，我们向 NIA 申请 R01 资助的研究补充，并提交该资助以回应 PA-21-071 旨在促进科学研究队伍的多样性并发展 我们提出了一个详细的计划来促进符合条件的研究人员的职业生涯。 肯尼莉亚·梅兰森 (Kennelia Mellanson) 是该实验室的一名研究生，目前的项目重点是小说。 确定心室和类淋巴系统关键组成部分的定量方法 大脑中的运输——纤毛跳动、脑脊液（CSF）的定向流动以及 大脑附睾层的结构——对于心室有效运输至关重要的特征 脑脊液 (CSF) 和水通道蛋白 4 (AQP4) 通道的结构，水通道蛋白 4 (AQP4) 通道是 类淋巴运输系统。 我们的提案与 NIA“脑淋巴和脑脊液系统”最初的 R01 拨款完全一致 衰老和阿尔茨海默病”，重点关注脑组织稳态机制 我们现在建议在心室中使用我们的新技术和发现。 和类淋巴运输，以确定伴随衰老和进展的变化 小鼠模型中的阿尔茨海默病，并使用该项目作为帮助和指导的平台 我们详细介绍了肯尼莉亚·梅兰森 (Kennelia Mellanson) 作为研究员和学者的职业生涯。 Kennelia 的专业发展计划，包括详细的指导计划和时间表， 手稿和赠款写作、教学活动、提高演讲技巧、参与 此外，我们提出了研究关键的研究计划。 运输系统的组成部分可能会因衰老和阿尔茨海默病而受到损害。

项目成果

Spatiotemporal 3D image registration for mesoscale studies of brain development.

• DOI: 10.1038/s41598-022-06871-8
• 发表时间: 2022-03-07
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Shuvaev S;Lazutkin A;Kiryanov R;Anokhin K;Enikolopov G;Koulakov AA
• 通讯作者: Koulakov AA

The bioavailability time of commonly used thymidine analogues after intraperitoneal delivery in mice: labeling kinetics in vivo and clearance from blood serum.

• DOI: 10.1007/s00418-021-02048-y
• 发表时间: 2022-02
• 期刊: HISTOCHEMISTRY AND CELL BIOLOGY
• 影响因子: 2.3
• 作者: Maltsev, Dmitry I.;Mellanson, Kennelia A.;Belousov, Vsevolod V.;Enikolopov, Grigori N.;Podgorny, Oleg V.
• 通讯作者: Podgorny, Oleg V.

Physiology of Glymphatic Solute Transport and Waste Clearance from the Brain.

• DOI: 10.1152/physiol.00015.2022
• 发表时间: 2022-11-01
• 期刊: Physiology (Bethesda, Md.)
• 作者: Zhao, Lucy;Tannenbaum, Allen;Benveniste, Helene
• 通讯作者: Benveniste, Helene

Age-related decline in cognitive flexibility is associated with the levels of hippocampal neurogenesis.

• DOI: 10.3389/fnins.2023.1232670
• 发表时间: 2023
• 期刊: FRONTIERS IN NEUROSCIENCE
• 影响因子: 4.3
• 作者: Amelchenko, Evgeny M.;Bezriadnov, Dmitri V.;Chekhov, Olga A.;Anokhin, Konstantin V.;Lazutkin, Alexander A.;Enikolopov, Grigori
• 通讯作者: Enikolopov, Grigori

NTnC-like genetically encoded calcium indicator with a positive and enhanced response and fast kinetics.

• DOI: 10.1038/s41598-018-33613-6
• 发表时间: 2018-10-15
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Barykina NV;Doronin DA;Subach OM;Sotskov VP;Plusnin VV;Ivleva OA;Gruzdeva AM;Kunitsyna TA;Ivashkina OI;Lazutkin AA;Malyshev AY;Smirnov IV;Varizhuk AM;Pozmogova GE;Piatkevich KD;Anokhin KV;Enikolopov G;Subach FV
• 通讯作者: Subach FV