Integrated Microscopy

集成显微镜

基本信息

批准号：
10379982
负责人：
BENJAMIN S GLICK
金额：
$ 9.57万
依托单位：
UNIVERSITY OF CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-09-01 至 2024-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10379982
关键词：
Ablation Accounting Animal Organ Biological Sciences Budgets Buffers Cancer Center Support Grant Cells Chicago Color Comprehensive Cancer Center Computer Workstations Computer software Confocal Microscopy Data Data Analyses Development Dimensions Ensure Equipment Experimental Designs Fluorescence Fostering Funding Hour Image Image Analysis Individual Laboratories Lasers Light Lighting Maintenance Malignant Neoplasms Medicine Methodology Microscope Microscopy Modernization Molecular NCI Center for Cancer Research Optics Physiological Physiological Processes Preparation Publications Recovery Research Research Personnel Resolution Resource Sharing Resources Services Site Specific qualifier value Speed Structure Supervision System Techniques Technology Testing Training Universities University resources Work anticancer research base cost education resources equipment acquisition imaging capabilities imaging software instrument instrumentation light microscopy member microscopic imaging millimeter nanometer operation tool translational cancer research

Ablation Accounting Animal Organ Biological Sciences Budgets Buffers Cancer Center Support Grant Cells Chicago Color Comprehensive Cancer Center Computer Workstations Computer software Confocal Microscopy Data Data Analyses Development Dimensions Ensure Equipment Experimental Designs Fluorescence Fostering Funding Hour Image Image Analysis Individual Laboratories Lasers Light Lighting Maintenance Malignant Neoplasms Medicine Methodology Microscope Microscopy Modernization Molecular NCI Center for Cancer Research Optics Physiological Physiological Processes Preparation Publications Recovery Research Research Personnel Resolution Resource Sharing Resources Services Site Specific qualifier value Speed Structure Supervision System Techniques Technology Testing Training Universities University resources Work anticancer research base cost education resources equipment acquisition imaging capabilities imaging software instrument instrumentation light microscopy member microscopic imaging millimeter nanometer operation tool translational cancer research

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项目摘要

ABSTRACT The Integrated Microscopy Facility (IMF) functions as a supervised, user-based Core providing state-of-the-art microscopy imaging capabilities to University of Chicago Medicine Comprehensive Cancer Center (UCCCC) members and other University investigators. Light microscopy is an essential technology for cancer investigators. Modern microscopy enables observation of ever-finer detail, now breaking the optical diffraction barrier to yield super-resolution detail, thus providing information at scales ranging from tens of millimeters to less than 10 nanometers. The IMF offers all major wide-field and confocal techniques in addition to super- resolution technologies (e.g., stimulated emission depletion (STED), localization, structured light), and works to advance these methodologies for application to cancer research. Specific IMF developments in the current funding period include significant upgrades to microscope technologies and data analysis tools. Notably, these include installation of GSD super-resolution, a Lightsheet.z1 system, a SP8 white-light-laser spectral confocal, additional high-end workstations and upgrade to full-feature Imaris software. IMF staff additionally provides equipment maintenance, user training, and expert advice. Budgets reflect strictly a cost-recovery operation, with all services provided “at-cost”. As an integrated Core, IMF allows for efficient pooling of resources and management, while creating a large user base that aids in leveraging support from the University as well as other agencies and keeping costs low. Half of the IMF usage is associated with research conducted by UCCCC members. Specifically, UCCCC labs are major users of the IMF; for any given service that approaches capacity usage, every effort is made to ensure priority access for UCCCC investigators. Institutional support mechanisms significantly enhance UCCCC research efforts within the IMF. The Biological Science Division (BSD) invested $1,001,408 in the Core in 2016 alone to purchase equipment requested by UCCCC members. The Core supported the work of 99 UCCCC investigators in the current funding period (2013-2016), which included 647 individual users of IMF services.

抽象的集成显微镜设施（IMF）充当有监督的，基于用户的核心，提供最先进的芝加哥大学医学综合癌症中心（UCCCC）的显微镜成像能力成员和其他大学调查员。光学显微镜是癌症的重要技术调查人员。现代显微镜可以观察到详细的细节，现在打破光学衍射产生超分辨率细节的障碍，从而提供从数十毫米到的尺度提供信息小于10纳米。 IMF除了超级 - 分辨率技术（例如，刺激的发射耗竭（SteD），定位，结构化光），并致力于推进这些方法用于癌症研究。当前的特定IMF发展资金期包括对显微镜技术和数据分析工具的重大升级。值得注意的是包括安装GSD超级分辨率，Lightsheet.z1系统，SP8白色灯光光谱共焦，其他高端工作站并升级到全功能Imaris软件。国际货币基金组织的工作人员还提供设备维护，用户培训和专家建议。预算严格反映了成本恢复操作，所有服务提供了“成本”。作为集成核心，IMF允许有效地汇集资源和管理，同时创建一个庞大的用户群，以帮助利用大学的支持以及其他机构并保持成本较低。 IMF使用的一半与研究有关 UCCCC成员。具体而言，UCCCC实验室是IMF的主要用户。对于任何接近的给定服务容量使用，尽一切努力确保UCCCC调查人员的优先访问。机构支持机制大大提高了IMF内的UCCCC研究工作。生物科学系（BSD）仅在2016年向核心投资了1,001,408美元，以购买UCCCC成员要求的设备。核心支持当前资助期（2013-2016）的99名UCCCC调查人员的工作，包括647个IMF服务的个人用户。