Developing a new chordate model for stem cell biology and regeneration

开发用于干细胞生物学和再生的新脊索动物模型

• 批准号: 10373777
• 负责人: Anthony W De Tomaso
• 金额: $ 18.39万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA BARBARA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-03-15 至 2024-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10373777
• 关键词: Adult; Animal Model; Animals; Biology; Blood Vessels; CRISPR/Cas technology; Cardiovascular system; Cell Differentiation process; Cell Lineage; Cells; Characteristics; Chordata; Databases; Development; Embryo; Embryonic Development; Endocrine; Gastrointestinal tract structure; Genes; Genome; Genomics; Germ Cells; Germ Layers; Glass; Goals; Grant; Heart; Human; Image; Invertebrates; Modeling; Modification; Natural regeneration; Nervous system structure; Neural Crest; Organ; Organism; Pattern; Peripheral Nervous System; Phylogenetic Analysis; Process; Regenerative pathway; Regenerative research; Reporter; Research; Resolution; STEM field; Series; Signal Transduction; Sister; Slide; Source; Stem cell transplant; Study models; Testing; Time; Tissues; Vertebrates; ascidian; asexual; body system; cardiac regeneration; cardiogenesis; cell regeneration; cell type; gene regulatory network; improved; innovation; life history; loss of function; notochord; novel; progenitor; regenerative; regenerative biology; response; self-renewal; single-cell RNA sequencing; stem cell biology; stem cell model; stem cells; tissue regeneration; tool; transcription factor

ABSTRACT The goal of this grant is to adapt contemporary single cell resolution and genomic manipulation tools to utilize the unique regenerative biology of the colonial ascidian species, Botryllus schlosseri. The FOA we are responding to from ORIP has as its stated goal to create new and innovative models for stem cell biology and regeneration, and Botryllus has a proven track record of innovative studies and a wealth of biology that will be made accessible with these new tools. Ascidians are chordates, and considered to be the closest living relatives to the vertebrates. Species such as Ciona have become powerful models in dissecting early steps in chordate embryogenesis due to their relative simplicity and rapid development, and share many chordate-specific features, including early mechanisms underlying cell specification, as well as clonal origins of multiple tissues. In addition to this close phylogenetic relationship, a subset of ascidian species are colonial, and grow, not by increasing in size, but via a process of regeneration- whereby entire bodies- including all cardiovascular, nervous, endocrine and germline tissues- are regenerated, and in Botryllus this occurs every week in a synchronized fashion, providing a novel model to study regeneration of all major organ systems in a chordate. Colonial ascidians are the only chordates with the ability to regenerate every tissue and organ in their body and unique life history characteristics make Botryllus a potentially outstanding model for these studies.

抽象的 这项赠款的目的是适应当代的单细胞分辨率和基因组操纵 利用殖民地海豚物种Botryllus的独特再生生物学的工具 Schlosseri。我们正在从Orip回应的FOA作为其既定的目标，即创建新的目标 干细胞生物学和再生的创新模型和botryllus具有验证的轨道 创新研究和大量生物学的记录，这些新的新型 工具。西班牙人是弦，被认为是最接近的亲戚 脊椎动物。诸如ciona之类的物种已成为剖析早期步骤的强大模型 核核酸的胚胎发生由于它们的相对简单性和快速发展，并共享许多 脊髓特异性特征，包括细胞规范的早期机制，以及 多个组织的克隆起源。除了这种紧密的系统发育关系外， 海沿物种是殖民地的，而不是通过增加的大小，而是通过 再生 - 整个身体（包括所有心血管，紧张，内分泌和 种系组织是再生的，在botryllus中，每周都会在同步中发生 时尚，提供了一种新型模型来研究弦园中所有主要器官系统的再生。 殖民时期的海沿岸是仅有的弦，具有再生每个组织和器官的能力 他们的身体和独特的生活历史特征使博特利洛斯成为潜在的杰出模型 对于这些研究。