Molecular epidemiology and serology of malaria under shifting agricultural practices in Africa

非洲农业实践转变下疟疾的分子流行病学和血清学

• 批准号: 10308046
• 负责人: Elizabeth Anne Hemming-Schroeder
• 金额: $ 3.83万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-12-01 至 2022-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10308046
• 关键词: Accounting; Affect; Africa; Africa South of the Sahara; African; Agriculture; Antibody Response; Antigens; Area; Bioinformatics; Biological Markers; Blood; Breeding; Cessation of life; Chronic; Clinic; Clinical; Clinical Research; Country; Coupled; Cross-Sectional Studies; Culicidae; Data; Detection; Development; Disease Surveillance; Drug resistance; Environment; Epidemiology; Faculty; Famines; Farm; Fellowship; Funding; Future; Genetic; Genetic Markers; Genetic Structures; Genetic Variation; Genotype; Goals; Habitats; Health; Hospitals; Human; Hunger; Immunologic Techniques; Immunology; Incidence; Infection; Interdisciplinary Study; International; Investments; Irrigation; Kenya; Knowledge; Lead; Leadership; Longitudinal cohort study; Malaria; Malnutrition; Measures; Mentorship; Microsatellite Repeats; Migrant; Modeling; Molecular Epidemiology; Monitor; Movement; Outcome; Parasite resistance; Parasites; Plasma; Plasmodium falciparum; Population; Population Genetics; Population Growth; Population Heterogeneity; Positioning Attribute; Predisposition; Public Health; Questionnaires; Recording of previous events; Research; Research Activity; Research Personnel; Research Training; Resource Development; Risk; Salivary Proteins; Scheme; Secure; Serology; Site; Sporozoites; Testing; Time; Training; Travel; Universities; Ursidae Family; Water; Work; World Health Organization; burden of illness; career; combat; deep sequencing; design; epidemiology study; experience; exposed human population; food insecurity; food security; genetic makeup; genomic data; global health; human migration; improved; insight; land cover; land use; malaria transmission; next generation sequencing; population genetic structure; population movement; professor; programs; remote sensing; serological marker; skills; spatiotemporal; vector

Project Summary This is an application for a F32 fellowship for Dr. Elizabeth Hemming-Schroeder, a Postdoctoral Scholar proposing to train under the sponsorship of Dr. James Kazura, Professor of Global Health at Case Western Reserve University. The overall goal for this fellowship is for Dr. Hemming-Schroeder to cultivate her skills to become a future independent investigator in the field of malaria epidemiology. Specifically, this F32 fellowship will provide Dr. Hemming-Schroeder with the necessary support to achieve the following goals: 1) Design epidemiological studies to test hypotheses related to malaria transmission and control; 2) Analyze large sets of genomic data using bioinformatics; 3) Use immunological techniques to study malaria epidemiology; and 4) Develop leadership and professional skills to lead an interdisciplinary research team. To combat food insecurity and meet increasing water demands under rapid population growth, water resource development projects are anticipated to rapidly increase in sub-Saharan Africa. The impacts that these water resource development projects will have on malaria risk remain poorly understood. This project will focus on assessing the impacts of shifting agricultural practices and human migration resulting from irrigation projects on parasite population genetics and malaria epidemiology. In newly irrigated and non-irrigated areas of Kenya, Dr. Hemming-Schroeder will monitor land use and population movement and use cross-sectional surveys, a longitudinal cohort study, and passive case surveillance to monitor changes in parasite populations in local residents and migrant laborers and malaria incidence in the areas over time. In Aim 1, Dr. Hemming-Schroeder will examine the impacts of shifting agricultural practices on parasite population genetics and diversity through genotyping of microsatellite and drug resistance markers, as well as amplicon next-generation sequencing and bioinformatic analysis. In Aim 2, Dr. Hemming-Schroeder will determine the impacts of shifting agricultural practices on symptomatic disease burden, parasite exposure history, and infection complexity through passive case detection in local health centers and hospitals, serology for biomarkers of parasite exposure, bioinformatic analysis of deep sequences, and mixed effects model analysis. This research will help to improve our understanding of the impacts water resource development projects have on malaria epidemiology and population genetics, which is critically important to being able to maintain effective malaria control programs. Data from this research will serve as preliminary data for a K22 or K99 application in the final year of the F32 fellowship. Through the proposed research activities and training plan, Dr. Hemming-Schroeder will gain skills in epidemiology, bioinformatics, immunology, and leadership and professional skills, which will help her to gain independent funding and prepare her for a faculty career leading an interdisciplinary research program.

项目摘要 这是博士后学者伊丽莎白·海明·斯罗德（Elizabeth Hemming-Schroeder）博士的F32奖学金的申请 提议在Case Western全球健康教授James Kazura博士的赞助下进行培训 预备大学。该团契的总体目标是Hemming-Schroeder博士培养自己的技能 成为疟疾流行病学领域的未来独立研究者。具体而言，这个F32奖学金 将为Hemming-Schroeder博士提供必要的支持以实现以下目标：1）设计 检验与疟疾传播和控制有关的假设的流行病学研究； 2）分析大型集 使用生物信息学的基因组数据； 3）使用免疫学技术研究疟疾流行病学；和4） 发展领导和专业技能以领导跨学科研究团队。打击食物不安全感 在人口迅速增长下，满足越来越多的水需求，水资源开发项目是 预计在撒哈拉以南非洲会迅速增加。这些水资源开发的影响 项目将在疟疾风险方面的理解不足。该项目将着重于评估 由寄生虫种群灌溉项目引起的农业实践和人类移民 遗传学和疟疾流行病学。在肯尼亚的新灌溉和未灌溉的地区，Hemming-Schroeder博士 将监视土地使用和人口流动，并使用横断面调查，一项纵向队列研究以及 被动案件监视以监视当地居民和移民劳动者的寄生虫种群的变化以及 随着时间的流逝，该地区的疟疾发病率。在AIM 1中，Hemming-Schroeder博士将检查转移的影响 寄生虫种群遗传学和多样性的农业实践通过微卫星和药物的基因分型 电阻标记以及扩增子的下一代测序和生物信息学分析。在AIM 2中，博士 出身术语将确定转移农业实践对有症状疾病负担的影响， 寄生虫暴露历史和通过当地卫生中心被动案例检测的感染复杂性 医院，寄生虫暴露生物标志物的血清学，深层序列的生物信息分析和混合 效应模型分析。这项研究将有助于提高我们对影响水资源影响的理解 开发项目对疟疾流行病学和人群遗传学有很重要的 能够维持有效的疟疾控制计划。这项研究的数据将作为初步数据 在F32奖学金的最后一年中，用于K22或K99的申请。通过拟议的研究活动以及 培训计划，Hemming-Schroeder博士将获得流行病学，生物信息学，免疫学和 领导和专业技能，这将有助于她获得独立的资金并为教师做好准备 职业领导跨学科研究计划。

项目成果

Widespread multiple insecticide resistance in the major dengue vector Aedes albopictus in Hainan Province, China.

中国海南省主要登革热媒介白纹伊蚊普遍存在多种杀虫剂抗药性。

• DOI: 10.1002/ps.6222
• 发表时间: 2021-04
• 期刊: Pest management science
• 影响因子: 4.1
• 作者: Li Y;Zhou G;Zhong D;Wang X;Hemming-Schroeder E;David RE;Lee MC;Zhong S;Yi G;Liu Z;Cui G;Yan G
• 通讯作者: Yan G