The neuroethology of feeding in jellyfish

水母摄食的神经行为学

基本信息

批准号：
17F17092
负责人：
出口竜作
金额：
$ 1.47万
依托单位：
Miyagi University of Education
依托单位国家：
日本
项目类别：
Grant-in-Aid for JSPS Fellows
财政年份：
2017
资助国家：
日本
起止时间：
2017-04-26 至 2019-03-31
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17F17092/
关键词：
Neurobiology Cnidarian Behavior Jellyfish Feeding Functional labeling Transcriptomics

项目摘要

1.1. Neuronal activity mapping in the jellyfishTo identify neurons controlling feeding, we used phospho-MAPK antibody staining in the jellyfish. Typically, when a neuron fires an action potential, calcium flows into the cell, causing activation of the MAPK/ERK signaling pathway, which in turn triggers MAPK phosphorylation into phospho-MAPK (pMAPK). Thus, by staining whole jellyfish eith a pMAPK antibody, we can identify activated neurons. To prove that this approach works, we stimulated jellyfish with light and compared the pMAPK staining with jellyfish which remained in darkness. This resulted in strong staining of the neurons around the jellyfish eyes, but only for the animals that were exposed to light. This proves that anti-pMAPK staining can be used for labeling active neurons in jellyfish. Unfortunately, we were unable to identify a clear pattern when we used other sensory modalities, such as chemical or mechanical stimulation.1.2. Identification of genes involved in jellyfish feedingTo better understand the genes involved in the regulation of jellyfish feeding, we carried out RNA analyses in hungry and fed animals. In this way, we were able to identify hundreds of genes that become more highly expressed when the jellyfish is hungry or full. Our hypothesis is that some of these genes may be involved in the regulation of appetite in jellyfish. Using bioinformatics approaches, we were able to identify potentially conserved genes that also play a role in appetite regulation in other animals.

1.1.水母中的神经元活动图谱为了识别控制摄食的神经元，我们在水母中使用了磷酸-MAPK 抗体染色。通常，当神经元激发动作电位时，钙流入细胞，引起 MAPK/ERK 信号通路激活，进而触发 MAPK 磷酸化为磷酸化 MAPK (pMAPK)。因此，通过用 pMAPK 抗体对整个水母进行染色，我们可以识别激活的神经元。为了证明这种方法有效，我们用光刺激水母，并将 pMAPK 染色与保持在黑暗中的水母进行比较。这导致水母眼睛周围的神经元被强烈染色，但仅限于暴露在光线下的动物。这证明抗pMAPK染色可用于标记水母中的活性神经元。不幸的是，当我们使用其他感觉方式（例如化学或机械刺激）时，我们无法识别清晰的模式。1.2。鉴定参与水母摄食的基因为了更好地了解参与调节水母摄食的基因，我们对饥饿和进食动物进行了 RNA 分析。通过这种方式，我们能够识别出数百个基因，当水母饥饿或饱足时，这些基因的表达会更高。我们的假设是，其中一些基因可能与水母食欲的调节有关。利用生物信息学方法，我们能够识别出在其他动物的食欲调节中也发挥作用的潜在保守基因。