Research Supplements to Promote Diversity

促进多样性的研究补充

• 批准号: 10281488
• 负责人: Mehmet Remzi Dokmeci
• 金额: $ 3.99万
• 依托单位: TERASAKI INSTITUTE FOR BIOMEDICAL INNOVATION
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10281488
• 关键词: Address; Animal Model; Architecture; Behavior; Binding; Biological Assay; Biological Markers; Biophotonics; Biosensor; Cardiac; Charge; Clinical; Clinical Trials; Crystallization; Detection; Development; Drug Industry; Drug toxicity; Economics; Event; Failure; Funding; Goals; Grant; Heart; Human; Image; In Situ; Kinetics; Knowledge; Label; Laboratories; Minority; Modeling; Monitor; Optics; Organ Model; Parents; Pharmaceutical Preparations; Physiology; Research; Research Activity; Research Institute; Sampling; Students; Surface; System; Talents; Texas; Time; Tissue Microarray; Tissue Model; Training; United States National Institutes of Health; Universities; Work; Writing; career; design; doctoral student; drug candidate; human tissue; imaging system; improved; indexing; interest; minority student; multiplex detection; organ on a chip; parent project; photonics; response; sensor; side effect; skills; training opportunity

Project Summary This proposal is to apply for a supplement grant to support a talented, minority Ph.D. student, Frank DeLuna, to develop a new label-free imaging system for drug toxicity assays in Dr. Jing Yong Ye’s laboratory at the University of Texas at San Antonio. Accurate analysis of drug toxicities is in urgent demand as failures of newly discovered drug candidates due to toxic side effects have resulted in about 30% of clinical attrition, leading to enormous economic losses for the pharmaceutical industry. One of the main reasons for these failures can be attributed to the fact that most animal models fail to accurately reflect human physiology. Therefore, there is a significant interest in constructing human organ models for drug toxicity assays before proceeding with clinical trials. For that, it is critically important not only to construct small tissue chips that can well mimic the architecture and functionality of human tissues, but also to continuously monitor the dynamic behaviors of human organ-on-a-chip models in response to drugs in situ over an extended period. The overarching goal of the parent R01 project (1R01GM126571) is to address the challenging issues of drug toxicity assays by using a human organ-on-a-chip model monitored with an automated, label-free, optical biosensor system that allows for real-time, long-term, sensitive, and kinetic analyses of human cardiac tissue models in response to various drugs in their microenvironments. The proposed research activities for the student under this supplement grant is to develop a new label-free imaging system by utilizing a photonic crystal biosensor in a unique detection mode for accurate quantification of biomarkers due to drug toxicities. The proposed work is related to Aim 2 in the parent project, and it will also create a new capability for directly imaging samples in the evanescent field of the sensor for simultaneously monitoring reflective index changes in each sample well due to binding events of biomarkers secreted by a heart-on-a-chip model with biorecognition probes on the sensor surface. This new design will offer a good alternative approach for multiplex detection of biomarkers in drug toxicity assays. This supplement grant will offer an excellent training opportunity for the minority student to gain deep knowledge and necessary research skills in biophotonics as well as to improve his other capabilities, such as writing and networking skills, preparing him for a successful academic career.

项目摘要 该建议是申请支持奖学金，以支持有才华的少数族裔学生， 弗兰克·德鲁纳（Frank Deluna Yong Ye在得克萨斯大学圣安东尼奥大学的实验室。 由于有毒的副作用而导致新发现的候选毒品的失败有迫切需求 导致约30％的临床流失，导致您的经济损失巨大 制药行业。 大多数动物模型无法准确反映人类的生理学。 在进行之前为药物毒性建造人体器官模型的重大兴趣 对此进行临床试验。 很好地模仿人体组织的结构和功能，但也可以连续监测 人类在芯片模型中的动态行为是对药物的原位呼吸的动态行为 延长时期。 通过使用人类的芯片模型监控的药物毒性测定的挑战性问题 具有自动化，无标签的光学生物传感器系统，可实时，长期， 人类心脏组织模型的敏感和动力学分析，以响应各种药物 他们的微环境。 赠款是通过在A中利用光子晶体生物传感器来开发新的无标签成像系统 由于药物毒性而准确定量生物标志物 支撑工作与母公司项目中的AIM 2有关，这也将为 直接成像传感器的复发场中的样品，以同时监测 由于生物标志物的结合事件，每个样本的反射指数变化井中 具有生物识别探针在传感器表面上的片段模型将提供。 生物标志物在药物毒剂测定中的替代应用 补充赠款将为少数族裔学生提供极好的培训机会 知识和必要的研究技能以及改善他的其他 诸如写作和网络技能之类的能力为成功的学术生涯做好了准备。