Construction of the integrated human cortical organoids to investigate neurodevelopmental disorders

构建整合的人类皮质类器官来研究神经发育障碍

基本信息

批准号：
10216628
负责人：
In-Hyun Park
金额：
$ 63.9万
依托单位：
YALE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-09-09 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10216628
关键词：
3-Dimensional ASD patient Address Affect Animal Model Area Axon BMP7 gene Brain Brain imaging Brain region CRISPR/Cas technology Cell Line Cell Nucleus Cell physiology Cells Cellular Structures Chromatin Structure Clinical Complex Data Defect Development Diagnosis Disease Dorsal Fibroblast Growth Factor Ganglia Generations Genes Genetic Genetic Transcription Genetic study Genomics Goals Human Human Genetics Impairment Insulin Investigation Light Medial Mental disorders Methods Molecular Mutation Neurobiology Neurodevelopmental Disorder Neurons Organoids Outcome Patients Play Process Prosencephalon Proteins Reporting Role SHH gene Schizophrenia Signal Transduction Structure Subthalamic structure System Technology Thalamic structure Transcriptional Regulation WNT Signaling Pathway Work autism spectrum disorder base brain cell brain tissue cell type de novo mutation developmental disease diencephalon endophenotype human pluripotent stem cell imaging study improved migration neurodevelopment neuropsychiatric disorder protein complex rare variant self-renewal single-cell RNA sequencing species difference stem cells three dimensional structure tool transcriptome

3-Dimensional ASD patient Address Affect Animal Model Area Axon BMP7 gene Brain Brain imaging Brain region CRISPR/Cas technology Cell Line Cell Nucleus Cell physiology Cells Cellular Structures Chromatin Structure Clinical Complex Data Defect Development Diagnosis Disease Dorsal Fibroblast Growth Factor Ganglia Generations Genes Genetic Genetic Transcription Genetic study Genomics Goals Human Human Genetics Impairment Insulin Investigation Light Medial Mental disorders Methods Molecular Mutation Neurobiology Neurodevelopmental Disorder Neurons Organoids Outcome Patients Play Process Prosencephalon Proteins Reporting Role SHH gene Schizophrenia Signal Transduction Structure Subthalamic structure System Technology Thalamic structure Transcriptional Regulation WNT Signaling Pathway Work autism spectrum disorder base brain cell brain tissue cell type de novo mutation developmental disease diencephalon endophenotype human pluripotent stem cell imaging study improved migration neurodevelopment neuropsychiatric disorder protein complex rare variant self-renewal single-cell RNA sequencing species difference stem cells three dimensional structure tool transcriptome

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项目摘要

Project Summary Diagnosed mainly based on symptomatic description, it is difficult to distinguish the underlying mechanisms of neuropsychiatric disorders, including schizophrenia (SCZ) and autism spectrum disorders (ASD). Recent advanced human genetic studies have identified the genetic underpinning of the a variety of neuropsychiatric disorders, showing that majority of SCZ and ASD are genetically heterogeneous and caused by with rare de novo mutations. Particularly, a rare mutations at SET1/COMPASS complex proteins or related proteins were distinctly discovered at SCZ or ASD patients. Our central hypothesis is that investigating the function of SCZ- or ASD-associated proteins in cortical and subcortical development will reveal the molecular mechanism how mutations of proteins with similar catalytic activity result in clinically distinct disorders. The paucity of accessible human brain tissues has been challenging to directly addressing these questions by using human brain tissue. Thus, in order to achieve the goal, we will use human brain organoids that structually and functionally reproduce the developing human brain regions. The use of human pluripotent stem cells (hPSCs) has revolutionized the human brain studies. hPSCs undergo unlimited self-renewal and can differentiate into any cell types, including brain cells. We have reported the generation of 3-dimensional (3-D) structures from hPSCs that recapitulate the developing human cortex (hCO, cortical organoids), medial ganglionic eminence (hMGEO, MGE organoid), or diencephalic thalamus (hThO, thalamic organoids). Fusing hCO with hMGEO, or hThO reproduced the interaction of developmentally distinct two regions, such as tangential migration of MGE cells to cortex, or reciprocal corticothalamic or thalamocortical connections. Using the advanced stem cell tools, we will purse the aims to achieve the goal. 1) We will use CRISPR/CAS9 gene editing tools to introduce mutations of SCZ and ASD genes into hPSC lines, and investigate the cellular and molecular function in cortical and thalamic development using hThOs and hCOs. 2) We will develop methods to reproduce the multiple nuclei in thalamus to further improve the regional specification of hThOs. 3) We will investigate the function of SCZ and ASD genes in cortical and subcortical connectivity. Overall, our advanced human brain organoid-based approaches combined with genomics and neurobiological tools will define the molecular mechanism distinctly regulated by SCZ and ASD-associated genes and provide unprecedented unique platforms to construct the functional corticothalamic connection.

项目摘要主要根据症状描述进行诊断，很难区分神经精神疾病，包括精神分裂症（SCZ）和自闭症谱系障碍（ASD）。最近的先进的人类遗传研究已经确定了多种神经精神病学的遗传基础疾病，表明大多数SCZ和ASD都是遗传异质性的，并且由罕见的DE引起 Novo突变。特别是，set1/compass复合蛋白或相关蛋白质的罕见突变是在SCZ或ASD患者中明显发现。我们的中心假设是研究SCZ-的功能皮质和皮层发育中与ASD相关的蛋白质将揭示分子机制具有相似催化活性的蛋白质突变导致临床上不同的疾病。稀少人脑组织通过使用人脑组织直接解决这些问题一直具有挑战性。因此，为了实现目标，我们将使用结构和功能上的人类脑器官重现发展中的人脑区域。人类多能干细胞（HPSC）的使用具有彻底改变了人类脑研究。 HPSC经历无限的自我更新，可以区分到任何细胞类型，包括脑细胞。我们报道了从概括了发育中的人皮质（HCO，皮质器官）的HPSC，内侧神经节显着性（HMGEO，MGE类器官）或双脑丘脑（Htho，丘脑类器官）。将HCO与hmgeo融合，或他重现了发展上不同的两个区域的相互作用，例如MGE的切向迁移细胞到皮质，或相互的皮质丘脑或丘脑皮质连接。使用高级干细胞工具，我们将为实现目标的目标推动。 1）我们将使用CRISPR/CAS9基因编辑工具介绍 SCZ和ASD基因的突变成HPSC系，并研究细胞和分子功能使用HTHOS和HCOS的皮质和丘脑发育。 2）我们将开发复制的方法丘脑中的多个核进一步改善了Hthos的区域规范。 3）我们将调查 SCZ和ASD基因在皮质和皮层连通性中的功能。总体而言，我们先进的人类大脑基于器官的方法结合基因组学和神经生物学工具将定义分子由SCZ和与ASD相关的基因明显调节的机制，并提供前所未有的独特构建功能性皮质丘脑连接的平台。