Microscopy and Digital Imaging

显微镜和数字成像

• 批准号: 10216272
• 负责人: W MATTHEW PETROLL
• 金额: $ 22.31万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-30 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10216272
• 关键词: Address; Cells; Confocal Microscopy; Contract Services; Core Grant; Cytoskeleton; Data; Equipment; Extracellular Matrix; Fees; Funding; Future; Goals; Image; Image Analysis; Imaging Techniques; Imaging technology; Institution; Label; Laser Scanning Confocal Microscopy; Light; Maintenance; Microscopy; Process; Records; Research; Research Personnel; Research Project Grants; Resources; Scientist; Services; Structure; Technology; Time; Tissues; Training; Vision; Vision research; Visualization; cellular imaging; cost; cost effective; design; digital; digital imaging; equipment training; experimental study; image processing; image visualization; imaging facilities; imaging software; imaging study; immunocytochemistry; investigator training; microscopic imaging; morphometry; multiphoton microscopy; operation; quantitative imaging; reflectance confocal microscopy; tissue processing

Resource/Service Core: Microscopy and Digital Imaging Abstract: The Microscopy and Digital Imaging Module will provide equipment, infra-structural support, expertise and technical assistance to vision scientists in order to enable detailed, quantitative imaging of cells and tissues. This state-of-the-art imaging facility includes several pieces of high-end image acquisition and analysis hardware, and the expertise needed to support and enhance ongoing research and explore new research questions posed by vision scientists. Facility operations will include: 1) maintaining and servicing the equipment, 2) managing usage of the equipment, 3) educating investigators on the capabilities of the equipment, 4) advising investigators on the design of imaging experiments for vision research projects, 5) providing training on the proper usage of the equipment, and 6) helping researchers collect, process and analyze high quality microscopy data for both current and future applications. The specific services and technologies provided within the Microscopic Imaging Facility are: a) cell and tissue processing, labeling and immunocytochemistry, b) light and fluorescent microscopy, c) laser scanning confocal microscopy, d) multiphoton microscopy and time-lapse imaging, e) in vivo confocal microscopy, and f) quantitative morphometry and image analysis. Overall, the Microscopy and Digital Imaging Module will support a range of imaging technologies for participating investigators, and facilitate easy access to equipment, training and technical support that will expand experimental capabilities, open up new research directions, and result in more cost-effective, time- efficient and rigorous imaging studies. An additional goal is to facilitate collaborative studies using these cutting edge imaging techniques between vision scientists at UTSW and other institutions in the region.

资源/服务核心：显微镜和数字成像 抽象的： 显微镜和数字成像模块将提供设备，基础结构支持，专业知识和 对视力科学家的技术援助，以实现细胞和组织的详细定量成像。 该最先进的成像设施包括几片高端图像获取和分析 硬件以及支持和增强正在进行的研究并探索新研究所需的专业知识 视觉科学家提出的问题。设施操作将包括：1）维护和维护 设备，2）管理设备的使用，3）教育研究人员有关的功能 设备，4）为研究人员提供有关视觉研究项目的成像实验设计的建议，5） 提供有关设备适当使用的培训，以及6）帮助研究人员收集，流程和 分析当前和未来应用的高质量显微镜数据。 微观成像设施中提供的特定服务和技术是： A）细胞和组织加工，标记和免疫细胞化学， b）光和荧光显微镜， c）激光扫描共聚焦显微镜， d）多光子显微镜和延时成像， e）体内共聚焦显微镜和 f）定量形态计量学和图像分析。 总体而言，显微镜和数字成像模块将支持一系列成像技术 参与调查人员，并便利地获得设备，培训和技术支持 扩大实验能力，打开新的研究方向，并带来更具成本效益的时间 - 时间 有效而严格的成像研究。另一个目标是使用这些切割来促进协作研究 UTSW的视觉科学家与该地区其他机构之间的边缘成像技术。