Thermo Scientific Glacios cryo-TEM

Thermo Scientific Glacios 冷冻透射电镜

基本信息

批准号：
10175401
负责人：
Terunaga Nakagawa
金额：
$ 200万
依托单位：
VANDERBILT UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-05-15 至 2022-05-14
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10175401
关键词：
Acceleration Anabolism Architecture Arts Bacteria Biological Biomedical Engineering Calcium Signaling Cells Chemistry Clostridium difficile Collaborations Cryo-electron tomography Cryoelectron Microscopy Crystallization DNA Repair DNA biosynthesis Discipline Disease Electron Microscope Engineering Floor Funding Genome Human In Situ Inflammatory Investigation Ion Channel Ions Laboratories Ligands Link Lipids Macromolecular Complexes Maintenance Mediating Membrane Membrane Proteins Messenger RNA Micro Electron Diffraction Minor Molecular Molecular Conformation Molecular Motors Molecular Weight Nuclear Output Pathology Pharmaceutical Preparations Physiology Proteins Records Research Research Personnel Research Project Grants Resolution Resource Sharing Schools Science Signal Transduction Specimen Structural Biologist Structure Synapses Synaptic Transmission Synaptic Vesicles Synaptic plasticity Technology Therapeutic Toxin United States National Institutes of Health Vesicle Virus Assembly college design instrument interest medical schools novel particle protein function small molecule structural biology trafficking transmission process voltage

Acceleration Anabolism Architecture Arts Bacteria Biological Biomedical Engineering Calcium Signaling Cells Chemistry Clostridium difficile Collaborations Cryo-electron tomography Cryoelectron Microscopy Crystallization DNA Repair DNA biosynthesis Discipline Disease Electron Microscope Engineering Floor Funding Genome Human In Situ Inflammatory Investigation Ion Channel Ions Laboratories Ligands Link Lipids Macromolecular Complexes Maintenance Mediating Membrane Membrane Proteins Messenger RNA Micro Electron Diffraction Minor Molecular Molecular Conformation Molecular Motors Molecular Weight Nuclear Output Pathology Pharmaceutical Preparations Physiology Proteins Records Research Research Personnel Research Project Grants Resolution Resource Sharing Schools Science Signal Transduction Specimen Structural Biologist Structure Synapses Synaptic Transmission Synaptic Vesicles Synaptic plasticity Technology Therapeutic Toxin United States National Institutes of Health Vesicle Virus Assembly college design instrument interest medical schools novel particle protein function small molecule structural biology trafficking transmission process voltage

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项目摘要

PROJECT SUMMARY We propose to acquire a Thermo Scientific Glacios, a 200kV cryo-transmission electron microscope, to advance our biological investigation using the growing technologies of cryo-EM. The Glacios will be a part of the Cryo-EM Facility, a shared resource of the Center for Structural Biology. Our investigators have established records of using single particle analysis and discovering novel biological molecular mechanisms at the atomic level. The addition of the Glacios will be essential for our investigators to further advance their research beyond what is currently possible using our existing instruments. Specifically, the Glacios will be essential for: (1) structural analyses of low molecular weight proteins utilizing 200kV acceleration voltage, (2) the MicroED approach that uses electron diffraction of micro-crystals, (3) investigation of macromolecular complexes in situ using cryo- electron tomography, and (4) specimen optimization to advance our efficiency to resolve at high- resolution fine conformational varieties and small molecules, such as ligands, drugs, and ions. The technology provided by the Glacios is essential for breaking new ground on the NIH-funded research projects of the major users, which include studies of synaptic transmission and plasticity (Nakagawa), in situ synapse architecture and synaptic vesicle fusion (Zhou), Clostridioides difficile toxin pathology (Lacy), nuclear mRNA transport (Ren), DNA replication and repair (Eichmann), calcium signaling (Karakas), in situ viral assembly and architectures (Wan), vesicle and membrane trafficking (Jackson), and bacterial signaling (Iverson). Additionally, 11 minor users plan to exploit the Glacios to investigate critical biological questions in membrane protein function, inflammatory signaling, genome maintenance, bacteria-host interactions, ion channel mediated signaling, lipid signaling and biosynthesis, and molecular motors. The outputs of the research projects that will use the Glacios will substantially advance our basic understanding on the mechanisms of human physiology, pathology, and disease therapeutics. The Glacios will initially be used by structural biologists and cell biologists, with the userbase predicted to extend into the fields of chemistry and bioengineering who have related scientific interests. In fact, the Glacios will be housed in the Engineering and Science Building, which is designed to nurture collaborations between disciplines via a three-floor, integrative, and collaborative laboratory space linking the School of Medicine, College of Arts and Sciences, and the School of Engineering.

项目摘要我们建议购买Thermo Scientific Glacios，这是200 kV的低温传播电子显微镜，使用Cryo-EM的不断增长的技术来推进我们的生物学研究。冰川将是 Cryo-EM设施的一部分，这是结构生物学中心的共享资源。我们的研究人员已经建立了使用单个粒子分析并发现新颖的记录原子水平的生物分子机制。冰川的添加对于我们的调查人员将他们的研究进一步发展到目前的研究之外现有仪器。具体而言，冰川对：（1）低的结构分析至关重要。分子量蛋白利用200kV加速度电压，（2）使用的微型方法微晶的电子衍射，（3）使用冷冻 - 原位研究大分子复合物电子断层扫描和（4）标本优化，以提高我们在高效率上解决的效率分辨率细构象品种和小分子，例如配体，药物和离子。这冰川提供的技术对于在NIH资助的研究中打破新的基础至关重要主要用户的项目，包括突触传播和可塑性的研究（中川），原位突触体系结构和突触囊泡融合（Zhou），梭状芽胞杆菌艰难毒素病理学（蕾丝），核mRNA运输（REN），DNA复制和修复（Eichmann），钙信号传导（karakas），原位病毒组装和建筑（WAN），囊泡和膜贩运（杰克逊）和细菌信号传导（iverson）。此外，有11位次要用户计划利用冰川研究膜蛋白功能，炎症信号，基因组中的关键生物学问题维护，细菌宿主相互作用，离子通道介导的信号传导，脂质信号传导和生物合成和分子电机。使用冰川的研究项目的产出将大大提高了我们对人类生理，病理学机制的基本理解，和疾病疗法。冰川最初将被结构生物学家和细胞生物学家使用，预计用户群扩展到具有的化学和生物工程领域相关的科学利益。实际上，冰川将安置在工程和科学大楼中，旨在通过三层，综合和培养学科之间的合作与医学院，艺术与科学学院联系的合作实验室空间以及工程学院。