DEVELOPMENT OF FUNCTIONAL DENDORIMER FOR ANALYSIS OF BIORADICAL AND THE APPLICATION

生物自由基分析功能树枝状聚合物的研制及其应用

基本信息

批准号：
09480249
负责人：
OGATA Tateaki
金额：
$ 7.62万
依托单位：
YAMAGATA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

As one of the in vivo measurement methods for analyzing in vivo reaction of the bioradicals which are related to the physiological and disease phenomena of the living organism, the electron spin resonance (ESR) technique is extremely noticed. However, it is necessary to administer functional spin probe exogenously in order to apply this method to the in vivo measurement.Dendrimer is three-dimensional macromolecule. It is possible to give various functions to the dendrimer, because the introduction of the functional group to the center and the outer shell is easy. And, it is possible to retain the low weight molecular compound in the inside hole, because the inside is sparse on dendrimer with the rise of the generation, and because again, that terminal has the dense structure. In this study, it is a purpose to synthesize dendrimers containing spin probe in the inside and to obtain a basic knowledge on the function of the dendrimers.The dendrimer synthesized in this study included spin probe of about one molecule in the inside, and the water solubility and the organization recognition ability were expected since the end was modified by the sugar chain. Then, the dendrimer which included spin probe (Carboxy-PROXYL) was administered to a rat, and the dynamic behavior was observed on the rat skin. As the result, it was found that the life time of the spin probe was extended on about the double. Since the biocompatibility of the synthetic dendrimer was found to be very low, it was not possible to evaluate the organization recognition ability. The synthesis of dendrimer of the aliphatic polyether was tried in order to obtain the dendrimer of which the biocompatibility is higher. However, it is future problem on the property of the dendrimer.

作为分析与生物体的生理和疾病现象相关的生物自由基的体内反应的体内测量方法之一，电子自旋共振(ESR)技术备受关注。然而，为了将该方法应用于体内测量，需要外源施用功能性旋转探针。树枝状聚合物是三维高分子。由于将官能团引入中心和外壳很容易，因此可以赋予树枝状聚合物各种功能。并且，可以将低分子量化合物保留在内部孔中，因为随着代数的增加，内部在树枝状聚合物上变得稀疏，并且再次因为该末端具有致密的结构。本研究的目的是合成内部含有自旋探针的树枝状大分子，并获得有关树枝状大分子功能的基础知识。本研究合成的树枝状大分子内部含有约一个分子的自旋探针和水。由于末端被糖链修饰，因此具有预期的溶解度和组织识别能力。然后，将包含自旋探针(Carboxy-PROXYL)的树枝状聚合物给予大鼠，并观察大鼠皮肤上的动态行为。结果发现，自旋探针的寿命延长了大约两倍。由于发现合成树枝状聚合物的生物相容性非常低，因此无法评估组织识别能力。为了获得生物相容性更高的树枝状大分子，尝试了脂肪族聚醚树枝状大分子的合成。然而，树枝状聚合物的性能是未来的问题。