DEVELOPMENT OF FUNCTIONAL DENDORIMER FOR ANALYSIS OF BIORADICAL AND THE APPLICATION

生物自由基分析功能树枝状聚合物的研制及其应用

• 批准号: 09480249
• 负责人: OGATA Tateaki
• 金额: $ 7.62万
• 依托单位: YAMAGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09480249/
• 关键词: DENDRIMER; BIORADICAL; SPIN PROBE; ESR; IN VIVO; RAT; CARBOXY-PROXYL; ニトロキシルラジカル; アスコルビン酸; デンドリマ-; TEMPOL

As one of the in vivo measurement methods for analyzing in vivo reaction of the bioradicals which are related to the physiological and disease phenomena of the living organism, the electron spin resonance (ESR) technique is extremely noticed. However, it is necessary to administer functional spin probe exogenously in order to apply this method to the in vivo measurement.Dendrimer is three-dimensional macromolecule. It is possible to give various functions to the dendrimer, because the introduction of the functional group to the center and the outer shell is easy. And, it is possible to retain the low weight molecular compound in the inside hole, because the inside is sparse on dendrimer with the rise of the generation, and because again, that terminal has the dense structure. In this study, it is a purpose to synthesize dendrimers containing spin probe in the inside and to obtain a basic knowledge on the function of the dendrimers.The dendrimer synthesized in this study included spin probe of about one molecule in the inside, and the water solubility and the organization recognition ability were expected since the end was modified by the sugar chain. Then, the dendrimer which included spin probe (Carboxy-PROXYL) was administered to a rat, and the dynamic behavior was observed on the rat skin. As the result, it was found that the life time of the spin probe was extended on about the double. Since the biocompatibility of the synthetic dendrimer was found to be very low, it was not possible to evaluate the organization recognition ability. The synthesis of dendrimer of the aliphatic polyether was tried in order to obtain the dendrimer of which the biocompatibility is higher. However, it is future problem on the property of the dendrimer.

作为分析与生物体生理和疾病现象相关的生物自由基体体内反应的体内测量方法之一，电子旋转共振（ESR）技术非常注意。但是，为了将此方法应用于体内测量，必须外源性地施用功能性自旋探针。消化物二维是三维大分子。可以给树枝状聚合物提供各种功能，因为将功能组引入中心和外壳很容易。而且，可以将低重量分子化合物保留在内部孔中，因为内部在树枝状聚合物上稀疏，并且随着发电量的升高，并且因为它再次具有致密的结构。在这项研究中，目的是合成内部含有自旋探针的树状聚合物并获得有关树突聚合物功能的基本知识的目的。这项研究中合成的树突聚合物包括内部约一个分子的旋转探针，以及预期的水溶解性和组织识别能力，因为终点是由糖链修改的。然后，将包括自旋探针（羧基 - 羟基）的树突聚合物施用到大鼠，并在大鼠皮肤上观察到动态行为。结果，发现自旋探针的寿命大约在双重上延长。由于发现合成树状聚合物的生物相容性非常低，因此无法评估组织识别能力。为了获得生物相容性更高的树枝状聚合物的合成，尝试了脂肪族聚醚的树枝状聚合物。但是，这是树突聚合物属性的未来问题。