Development of Simulator for Vesicular Transport in Cells - Application to Study of Mechanism of Vesicular Transportation and Cell Locomotion.

细胞内囊泡运输模拟器的开发——在囊泡运输和细胞运动机制研究中的应用。

基本信息

批准号：
09558111
负责人：
KOSAWADA Tadashi
金额：
$ 5.63万
依托单位：
YAMAGATA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

A simulator for vesicular transport in cells was developed based on recent knowledge of molecular biology of the cell. Analytical method as well as finite element method were utilized to achieve the goal.1. Simulation of vesicular transport based on the analytical method(1) When the opening perimeter of a spherical membrane shell is unfolded with its perimeter kept parallel to its axis, there exists several points where the shapes and the tensions change significantly with slight changes of the opening radius. This is considered to be the bucking-like phenomena of the membrane with a spontaneous curvature.(2) In the repeating units of chained vesicles, it is found that the cylindrical tube shape and the hourglass shape are stable with respect to energy.(3) It is theoretically suggested that the chained vesicles and the cylindrical channel derived from the chained vesicles are some of the stable shapes with respect to energy and can potentially be formed and presented in the vascular en … More dothelial cell.(4) The computed shape suggests that the effects of in-plane shear elasticity and outer surrounding cytoplasmic membrane are significant and these should be taken into account in theoretical analysis.(5) The chained vesicle changes its shape dramatically even when the opening radius slightly increases from the starting shape. The jump-like phenomena of strain energy was often observed where each constriction disappears.2. Development of the specific finite element method for vesicular transport simulator by utilizing Mathematica(1) Formulation of the finite element method was developed based on elastic potential energy function. The requirement of constant surface area of the membrane was satisfied by implementing additional surface pressure.(2) The requirement of constant surface area of the membrane has significant effect on deformed shape of the vesicle.(3) Mooney-Rivlin type strain energy density function, which was developed for rubber red cell model, was utilized. The computed cortical tension was given as 0.003-0.18 dyn/cm. This results support the know experimental data of 0.03 dyn/cm. Less

基于细胞的分子知识开发了细胞中囊泡转运的模拟器。用平行的TOS轴展开，张力显着变化，而开口半径略有变化。稳定的形状相对于能量，可能会在血管EN中形成……在链式的囊泡中，应考虑更多的外质膜每个收缩消失 - 用于橡胶红细胞模型开发的晶体型应变密度函数被使用为0.003-0.18 dyn/cm。