Development of Simulator for Vesicular Transport in Cells - Application to Study of Mechanism of Vesicular Transportation and Cell Locomotion.

细胞内囊泡运输模拟器的开发——在囊泡运输和细胞运动机制研究中的应用。

• 批准号: 09558111
• 负责人: KOSAWADA Tadashi
• 金额: $ 5.63万
• 依托单位: YAMAGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09558111/
• 关键词: Vesicular Transport; Simulator; Bio-membrane; large Deformation Analysis; Analytical Method; Finite Element Method; Chained Vesicles; Outer Surrounding Cytoplasmic Membrane; 細胞内輸送; 膜小胞; 細胞移動; リポゾーム

A simulator for vesicular transport in cells was developed based on recent knowledge of molecular biology of the cell. Analytical method as well as finite element method were utilized to achieve the goal.1. Simulation of vesicular transport based on the analytical method(1) When the opening perimeter of a spherical membrane shell is unfolded with its perimeter kept parallel to its axis, there exists several points where the shapes and the tensions change significantly with slight changes of the opening radius. This is considered to be the bucking-like phenomena of the membrane with a spontaneous curvature.(2) In the repeating units of chained vesicles, it is found that the cylindrical tube shape and the hourglass shape are stable with respect to energy.(3) It is theoretically suggested that the chained vesicles and the cylindrical channel derived from the chained vesicles are some of the stable shapes with respect to energy and can potentially be formed and presented in the vascular en … More dothelial cell.(4) The computed shape suggests that the effects of in-plane shear elasticity and outer surrounding cytoplasmic membrane are significant and these should be taken into account in theoretical analysis.(5) The chained vesicle changes its shape dramatically even when the opening radius slightly increases from the starting shape. The jump-like phenomena of strain energy was often observed where each constriction disappears.2. Development of the specific finite element method for vesicular transport simulator by utilizing Mathematica(1) Formulation of the finite element method was developed based on elastic potential energy function. The requirement of constant surface area of the membrane was satisfied by implementing additional surface pressure.(2) The requirement of constant surface area of the membrane has significant effect on deformed shape of the vesicle.(3) Mooney-Rivlin type strain energy density function, which was developed for rubber red cell model, was utilized. The computed cortical tension was given as 0.003-0.18 dyn/cm. This results support the know experimental data of 0.03 dyn/cm. Less

基于细胞分子生物学的最新知识，开发了细胞中囊泡转运的模拟器。使用分析方法和有限元方法来实现目标1。当球形膜壳的开口周长与其轴平行保持时，囊泡转运的模拟基于分析方法（1）时，存在几个点，形状和紧张局势随着开口半径的略有变化而发生了很大变化。这被认为是带有赞助曲率的膜的类似弯曲的现象。（2）在链式蔬菜的重复单元中，发现圆柱管形状和沙漏形状稳定，而小型的蔬菜则相对于能量。 （4）计算的形状表明，平面剪切弹性和周围的细胞质膜外部的影响很大，应在理论分析中考虑这些效果。（5）当链式的整体变化急剧变化时，即使开放radius略微增加了从开放的启动形状，因此应考虑这些形状的变化。经常在每个收缩消失的地方观察到菌株能的跳跃现象。2。使用Mathematica（1）形成有限元方法的特定有限元方法是基于弹性势能函数开发的。通过实现额外的表面压力来满足膜的恒定表面积的需求。（2）膜的恒定表面积对囊泡的变形形状具有显着影响。（3）Mooney-Rivlin型应变能密度函数，该功能是为橡胶红细胞而开发的。计算出的皮质张力为0.003-0.18 dyn/cm。此结果支持0.03 Dyn/cm的知识实验数据。较少的

项目成果

Kosawada, Skalak et al.: "Formation of Chained Vesicle on Vascular Endothelial Cell and Its Dynamic Aspects" Proc. International Conf. on New Frontiers in Biomechanical Engineering. 85-88 (1997)

Kosawada、Skalak 等人：“血管内皮细胞链状囊泡的形成及其动态方面”Proc。

Kosawada, T., Skalak, R., Schmid-Schoenbein, G.W.: "Chained Vesicles in Vascular Endothelial Cells"ASME Journal of Biomechanical Engineering. 121-5. 472-479 (1999)

Kosawada, T.、Skalak, R.、Schmid-Schoenbein, G.W.：“血管内皮细胞中的链状囊泡”ASME 生物力学工程杂志。

Kosawada, Iijima, Kubota, Nakada, et al.: "A New Approach for Identification of Dynamic Properties of Mammalian Soft Tissues" Proc. International Conf. on New Frontiers in Biomechanical Engineering. 467-470 (1997)

Kosawada、Iijima、Kubota、Nakada 等人：“一种识别哺乳动物软组织动态特性的新方法”Proc。

Kosawada, Skalak, Yoshida, Schmid-Schoenbein: "Generation Mechanism of Vascular Endothelial Chained Vesicles and Transendothelial Channel"JSME Internationd Journal, Series C. 42・3. 796-803 (1999)

Kosawada、Skalak、Yoshida、Schmid-Schoenbein：“血管内皮链状囊泡和跨内皮通道的生成机制”JSME 国际期刊，系列 C. 796-803 (1999)。

Kosawada T., Takeno T., Skalak R., Schmid-Schonbein G.W.: "Formation of Chained Vesicle on Vascular Endothelial Cell and Its Dynamic Aspects"Proceedings of the International Conference on New Frontiers in Biomechanical Engineering, Tokyo. 85-88 (1997)

Kosawada T.、Takeno T.、Skalak R.、Schmid-Schonbein G.W.：“血管内皮细胞链状囊泡的形成及其动态方面”生物力学工程新前沿国际会议论文集，东京。