Bidirectional transport by ciliated epithelia : mechanism and implications

纤毛上皮的双向运输：机制和意义

• 批准号: 09640814
• 负责人: TAKAHASHI Keiichi
• 金额: $ 2.05万
• 依托单位: International Christian University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09640814/
• 关键词: Ciliated epithelia; echinoderm; counter-current mechanism; 繊毛運動; ウニ

The aim of this study was to investigate the possibility that bidirectiona1 transport that had been observed in the madreporite of echinoderms is an inherent property of ciliated epithelia in general. It also aimed to clarify the biological implications of this phenomenon , especially from the viewpoint of possible counter-current exchange mechanism. The transport of particles and water was studied mainly in isolated pore tubes of the sea urchin madreporite using colloidal carbon, polystyrene microbeads, and sulforhodamine 101, a water-soluble fluorescent dye. Observations were made under an inverted differential interference contrast and fluorescence microscope recording with a CCD video camera. The results showed that water is transported predominantly in the direction of the recovery stroke of the cilia while particulate matter was transported in the direction of the effective stroke. Thus, there seems to be a selective mechanism probably based on the size of the particle being transported. Basic data were obtained on the distribution of flow rate in the microscopic domains surrounding the cilia within the pore canals of the madreporite. Preliminary experiments were also carried out using other ciliated epithelia for similar analyses.

本研究的目的是调查在棘皮动物的 Madreporite 中观察到的双向运输是否是纤毛上皮的固有特性。它还旨在澄清这种现象的生物学意义，特别是从可能的逆流交换机制的角度来看。主要使用胶体碳、聚苯乙烯微珠和磺基罗丹明 101（一种水溶性荧光染料）在海胆 Madreporite 的孤立孔管中研究颗粒和水的传输。在倒置微分干涉和荧光显微镜下进行观察，并用 CCD 摄像机进行记录。结果表明，水主要沿纤毛恢复冲程的方向运输，而颗粒物质则沿有效冲程的方向运输。因此，似乎存在一种可能基于被传输颗粒的大小的选择机制。获得了有关 Madreporite 孔道内纤毛周围微观区域流速分布的基本数据。还使用其他纤毛上皮进行了初步实验以进行类似的分析。

项目成果

Braucker, R., Murakami, A., Ikegaya, K., Yoshimura, K., Takahashi, K., Machemer-Rohnisch, S., and Machemer, H.: "Relaxation and activation of graviresponses in Paramecium caudatum" J.Exp.Biol.201(14). 2103-2113 (1998)

Braucker, R.、Murakami, A.、Ikegaya, K.、Yoshimura, K.、Takahashi, K.、Machemer-Rohnisch, S. 和 Machemer, H.：“草履虫重力反应的松弛和激活”J.

Braucker,R.: "Relaxation and activation of graviresponses in Paramecium caudatum" J.Exp.Biol. 201・14. 2103-2113 (1998)

Braucker, R.：“尾草履虫的重力反应的松弛和激活”J.Exp.Biol. 201・14 2103-2113 (1998)。

Omoto, C.K., Gibbons, I.R., Kamiya, R., Shingyoji, C., Takahashi, K.and Witman, G.B.: "Rotation of the central pair microtubules in eukaryotic flagella" Mol.Biol.Cell.10(1). 1-4 (1999)

Omoto, C.K.、Gibbons, I.R.、Kamiya, R.、Shingyoji, C.、Takahashi, K. 和 Witman, G.B.：“真核鞭毛中中央微管对的旋转”Mol.Biol.Cell.10(1)。

Braucker, R.: "Relaxation and activation of graviresponses in Paramecium caudatum." J.Exp.Biol.201. 2103-2113 (1998)

Braucker, R.：“尾草履虫重力反应的松弛和激活。”

Takahashi, K.: "The movement of sea-urchin spines (in Japanese)" Comp.Physiol.Biochem.14(3). 185-193 (1997)

Takahashi, K.：“海胆刺的运动（日语）”Comp.Physiol.Biochem.14(3)。