Studies on diversity of sheep scrapie agents

羊痒病药剂多样性研究

基本信息

批准号：
09660312
负责人：
MATSUI Takane
金额：
$ 2.62万
依托单位：
Obihiro University of Agriculture and Veterinary Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

The agent (prion) of transmissible spongiform encephalopathies (prion diseases) including scrape in sheep, bovine spongiform encephalopathy, and Creutzfeldt-Jakob disease (CJD) in human does not possess the agent-specific nucleic acid as a genome. Therefore genome typing and/or antigenic typing are not applicable for distinction of prion strains. However, prion strains can be distinguishable to some extent by biological and biochemical properties. In this study, we attempted to elucidate whether diversity of sheep prion strains exist in Japan. In addition, we analyzed genetic factor(s) other than PrP which will influence biological properties of prions.Eleven sheep scrapie occurred in Japan were examined in the transmissibility to mice and the relative protease-resistance of sheep PrPィイD2scィエD2, accumulatedin brains. The results indicated that eleven sheep scrapie used here can be divided into three groups on the basis of biological and biochemical properties. This suggests that variou … More s sheep prions exist in the field of Japan. Although characterization of the biological properties of sheep prions in the field has been reported and revealed diversity of sheep prions in UK, this is the first report which elucidated the diversity of sheep prions in Japan. The results obtained here will be useful to assess the possible risk for the spread of sheep prion in Japan to other species.A particular genotype of apolipoprotein E (ApoE) is known as a risk factor for Alzheimer's disease. It is also suggested that a particular genotype of ApoE might influence the disease type of CJD. Thus we examined whether there exists any relation between sheep ApoE and prion diversity or disease type. First we cloned genomic DNA and cDNA of sheep ApoE gene and determined the nucleotide sequence of them, and we identified three sheep ApoE genotypes. However, there was no significant relationship between the polymorphism of sheep ApoE gene and biological properties of sheep prion or disease types. Less

可传播赞助商形式的脑病（prion疾病）的药物（包括绵羊刮擦，牛发起人形成脑病形成脑病）和人类中的Creutzfeldt-jakob病（CJD）在人类中没有假定药剂特异性核酸为基因组。因此，基因组分型和/或抗原分型不适用于剖析prion菌株。然而，通过生物学和生化特性可以在某种程度上区分prion菌株。在这项研究中，我们试图阐明日本是否存在绵羊pr菌菌株的多样性。此外，我们分析了除PRP以外的遗传因素，而PRP会影响王室的生物学特性。在日本进行了绵羊crapie crapie在传播中发生的绵羊小鼠和绵羊prpiy d2scie d2的相对蛋白酶抗性，累积的大脑积累。结果表明，这里使用的11只绵羊crap可以根据生物学和生化特性将三组分为三组。这表明Variou…在日本领域存在更多的绵羊prions。尽管据报道并揭示了英国绵羊王室在该领域的生物学特性的特征，但这是第一份阐明日本绵羊王室多样性的报告。此处获得的结果将有助于评估日本在其他特定的载脂蛋白E（APOE）中传播绵羊王室的可能风险，被称为阿尔茨海默氏病的危险因素。还提出，APOE的特定基因型可能会影响CJD的疾病类型。我们检查了绵羊Apoe与prion多样性或疾病类型之间是否存在任何关系。首先，我们克隆了绵羊APOE基因的基因组DNA和cDNA，并确定了它们的核苷酸序列，并确定了三种绵羊APOE基因型。但是，绵羊apoe基因的多态性与绵羊prion或疾病类型的生物学特性之间没有显着关系。较少的